Fabrication and Evaluation of a Micro(Bio)Sensor Array Chip for Multiple Parallel Measurements of Important Cell Biomarkers
This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-10-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/14/11/20519 |
| _version_ | 1797999141238865920 |
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| author | Roy M. Pemberton Timothy Cox Rachel Tuffin Guido A. Drago John Griffiths Robin Pittson Graham Johnson Jinsheng Xu Ian C. Sage Rhodri Davies Simon K. Jackson Gerry Kenna Richard Luxton John P. Hart |
| author_facet | Roy M. Pemberton Timothy Cox Rachel Tuffin Guido A. Drago John Griffiths Robin Pittson Graham Johnson Jinsheng Xu Ian C. Sage Rhodri Davies Simon K. Jackson Gerry Kenna Richard Luxton John P. Hart |
| author_sort | Roy M. Pemberton |
| collection | DOAJ |
| description | This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies. |
| first_indexed | 2024-04-11T10:59:52Z |
| format | Article |
| id | doaj.art-a4da000d433b417fae95ff935ed1e5aa |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T10:59:52Z |
| publishDate | 2014-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-a4da000d433b417fae95ff935ed1e5aa2022-12-22T04:28:38ZengMDPI AGSensors1424-82202014-10-011411205192053210.3390/s141120519s141120519Fabrication and Evaluation of a Micro(Bio)Sensor Array Chip for Multiple Parallel Measurements of Important Cell BiomarkersRoy M. Pemberton0Timothy Cox1Rachel Tuffin2Guido A. Drago3John Griffiths4Robin Pittson5Graham Johnson6Jinsheng Xu7Ian C. Sage8Rhodri Davies9Simon K. Jackson10Gerry Kenna11Richard Luxton12John P. Hart13Centre for Research in Biosciences, Faculty of Health and Life Sciences, University of the West of England, Bristol, BS16 1QY, UKQinetiQ Ltd., Malvern Technology Centre, Malvern, WR14 3PS, UKQinetiQ Ltd., Malvern Technology Centre, Malvern, WR14 3PS, UKApplied Enzyme Technology Ltd., Monmouth House, Mamhilad Park, Pontypool NP4 OHZ, UKUniscan Instruments Ltd., Sigma House, Burlow Rd., Buxton, Derbyshire SK17 9JB, UKGwent Electronic Materials Ltd., Monmouth House, Mamhilad Park, Pontypool NP4 OHZ, UKUniscan Instruments Ltd., Sigma House, Burlow Rd., Buxton, Derbyshire SK17 9JB, UKCentre for Research in Biosciences, Faculty of Health and Life Sciences, University of the West of England, Bristol, BS16 1QY, UKQinetiQ Ltd., Malvern Technology Centre, Malvern, WR14 3PS, UKQinetiQ Ltd., Malvern Technology Centre, Malvern, WR14 3PS, UKCentre for Research in Biosciences, Faculty of Health and Life Sciences, University of the West of England, Bristol, BS16 1QY, UKAstraZeneca R&D, Alderley Park, Macclesfield, SK10 4TF, UKInstitute of Biosensing Technology, University of the West of England, Bristol, BS16 1QY, UKCentre for Research in Biosciences, Faculty of Health and Life Sciences, University of the West of England, Bristol, BS16 1QY, UKThis report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies.http://www.mdpi.com/1424-8220/14/11/20519microbiosensorscreen-printingMEMS96-well platemetabolismamperometrycell monitoringtoxicity testing |
| spellingShingle | Roy M. Pemberton Timothy Cox Rachel Tuffin Guido A. Drago John Griffiths Robin Pittson Graham Johnson Jinsheng Xu Ian C. Sage Rhodri Davies Simon K. Jackson Gerry Kenna Richard Luxton John P. Hart Fabrication and Evaluation of a Micro(Bio)Sensor Array Chip for Multiple Parallel Measurements of Important Cell Biomarkers Sensors microbiosensor screen-printing MEMS 96-well plate metabolism amperometry cell monitoring toxicity testing |
| title | Fabrication and Evaluation of a Micro(Bio)Sensor Array Chip for Multiple Parallel Measurements of Important Cell Biomarkers |
| title_full | Fabrication and Evaluation of a Micro(Bio)Sensor Array Chip for Multiple Parallel Measurements of Important Cell Biomarkers |
| title_fullStr | Fabrication and Evaluation of a Micro(Bio)Sensor Array Chip for Multiple Parallel Measurements of Important Cell Biomarkers |
| title_full_unstemmed | Fabrication and Evaluation of a Micro(Bio)Sensor Array Chip for Multiple Parallel Measurements of Important Cell Biomarkers |
| title_short | Fabrication and Evaluation of a Micro(Bio)Sensor Array Chip for Multiple Parallel Measurements of Important Cell Biomarkers |
| title_sort | fabrication and evaluation of a micro bio sensor array chip for multiple parallel measurements of important cell biomarkers |
| topic | microbiosensor screen-printing MEMS 96-well plate metabolism amperometry cell monitoring toxicity testing |
| url | http://www.mdpi.com/1424-8220/14/11/20519 |
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