Genetic identification and comparative study on life history parameters of two strains belonging to Brachionus plicatilis species complex (Rotifera: Monogononta)
The rotifer Brachionus plicatilis is an important species for aquaculture, due to its use as food for bivalves, and fish and crustaceans larvae in hatcheries. However, being a species complex, it has become increasingly important to catalogue and describe the species and biotypes that constitute thi...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513422003052 |
| _version_ | 1828143826804408320 |
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| author | Luana Granada Marco F.L. Lemos Peter Bossier Sara C. Novais |
| author_facet | Luana Granada Marco F.L. Lemos Peter Bossier Sara C. Novais |
| author_sort | Luana Granada |
| collection | DOAJ |
| description | The rotifer Brachionus plicatilis is an important species for aquaculture, due to its use as food for bivalves, and fish and crustaceans larvae in hatcheries. However, being a species complex, it has become increasingly important to catalogue and describe the species and biotypes that constitute this complex. Therefore, the purpose of this study was to genetically identify two morphologically identical strains and evaluate their suitability to be used in aquaculture under the studied conditions. A correct identification and knowledge of life history characteristics of the biotypes and isolated strains is vital to avoid mass mortalities in aquaculture and to better interpret the responses of the organisms when these are used as a bioindicator and model for ecotoxicology. Strains MRS10 and IBA3 were identified as B. koreanus and, even though they have been maintained under the same laboratory conditions for several years, significant differences in several life history parameters were observed. A life table assay showed IBA3 rotifers to be larger at first reproduction, and to have longer post-reproductive period and mean lifespan. On the other hand, MRS10 rotifers produced less non-viable eggs and had higher population growth rate. Both strains showed to be a potential model for ecotoxicological and molecular studies, mainly due to the ease of maintenance, short generation time, and reproduction via parthenogenesis. However, MRS10 might present better characteristics than IBA3 to be reared in aquaculture as live food. |
| first_indexed | 2024-04-11T20:04:57Z |
| format | Article |
| id | doaj.art-a4e2d3026580440796fcb51d0ad1b79d |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-11T20:04:57Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-a4e2d3026580440796fcb51d0ad1b79d2022-12-22T04:05:21ZengElsevierAquaculture Reports2352-51342022-10-0126101309Genetic identification and comparative study on life history parameters of two strains belonging to Brachionus plicatilis species complex (Rotifera: Monogononta)Luana Granada0Marco F.L. Lemos1Peter Bossier2Sara C. Novais3MARE – Marine and Environmental Sciences Centre & ARNET – Aquatic Research Infrastructure Network Associated Laboratory, ESTM, Polytechnic of Leiria, 2520-641 Peniche, Portugal; Laboratory of Aquaculture & Artemia Reference Center, Department of Animal Sciences and Aquatic Ecology, Faculty of Bioscience Engineering, Ghent University, Ghent 9000, Belgium; Correspondence to: Edifício CETEMARES, Avenida do Porto de Pesca, 2520-630 Peniche, Portugal.MARE – Marine and Environmental Sciences Centre & ARNET – Aquatic Research Infrastructure Network Associated Laboratory, ESTM, Polytechnic of Leiria, 2520-641 Peniche, PortugalLaboratory of Aquaculture & Artemia Reference Center, Department of Animal Sciences and Aquatic Ecology, Faculty of Bioscience Engineering, Ghent University, Ghent 9000, BelgiumMARE – Marine and Environmental Sciences Centre & ARNET – Aquatic Research Infrastructure Network Associated Laboratory, ESTM, Polytechnic of Leiria, 2520-641 Peniche, Portugal; Correspondence to: Edifício CETEMARES, Avenida do Porto de Pesca, 2520-630 Peniche, Portugal.The rotifer Brachionus plicatilis is an important species for aquaculture, due to its use as food for bivalves, and fish and crustaceans larvae in hatcheries. However, being a species complex, it has become increasingly important to catalogue and describe the species and biotypes that constitute this complex. Therefore, the purpose of this study was to genetically identify two morphologically identical strains and evaluate their suitability to be used in aquaculture under the studied conditions. A correct identification and knowledge of life history characteristics of the biotypes and isolated strains is vital to avoid mass mortalities in aquaculture and to better interpret the responses of the organisms when these are used as a bioindicator and model for ecotoxicology. Strains MRS10 and IBA3 were identified as B. koreanus and, even though they have been maintained under the same laboratory conditions for several years, significant differences in several life history parameters were observed. A life table assay showed IBA3 rotifers to be larger at first reproduction, and to have longer post-reproductive period and mean lifespan. On the other hand, MRS10 rotifers produced less non-viable eggs and had higher population growth rate. Both strains showed to be a potential model for ecotoxicological and molecular studies, mainly due to the ease of maintenance, short generation time, and reproduction via parthenogenesis. However, MRS10 might present better characteristics than IBA3 to be reared in aquaculture as live food.http://www.sciencedirect.com/science/article/pii/S235251342200305216S rRNA geneAquacultureCryptic speciesEcotoxicologyFecundity and lifespanIntrinsic rate of population growth |
| spellingShingle | Luana Granada Marco F.L. Lemos Peter Bossier Sara C. Novais Genetic identification and comparative study on life history parameters of two strains belonging to Brachionus plicatilis species complex (Rotifera: Monogononta) Aquaculture Reports 16S rRNA gene Aquaculture Cryptic species Ecotoxicology Fecundity and lifespan Intrinsic rate of population growth |
| title | Genetic identification and comparative study on life history parameters of two strains belonging to Brachionus plicatilis species complex (Rotifera: Monogononta) |
| title_full | Genetic identification and comparative study on life history parameters of two strains belonging to Brachionus plicatilis species complex (Rotifera: Monogononta) |
| title_fullStr | Genetic identification and comparative study on life history parameters of two strains belonging to Brachionus plicatilis species complex (Rotifera: Monogononta) |
| title_full_unstemmed | Genetic identification and comparative study on life history parameters of two strains belonging to Brachionus plicatilis species complex (Rotifera: Monogononta) |
| title_short | Genetic identification and comparative study on life history parameters of two strains belonging to Brachionus plicatilis species complex (Rotifera: Monogononta) |
| title_sort | genetic identification and comparative study on life history parameters of two strains belonging to brachionus plicatilis species complex rotifera monogononta |
| topic | 16S rRNA gene Aquaculture Cryptic species Ecotoxicology Fecundity and lifespan Intrinsic rate of population growth |
| url | http://www.sciencedirect.com/science/article/pii/S2352513422003052 |
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