A Swollenin From Talaromyces leycettanus JCM12802 Enhances Cellulase Hydrolysis Toward Various Substrates
Swollenins exist within some fungal species and are candidate accessory proteins for the biodegradation of cellulosic substrates. Here, we describe the identification of a swollenin gene, Tlswo, in Talaromyces leycettanus JCM12802. Tlswo was successfully expressed in both Trichoderma reesei and Pich...
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.658096/full |
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| author | Honghai Zhang Honghai Zhang Yuan Wang Roman Brunecky Bin Yao Xiangming Xie Fei Zheng Huiying Luo |
| author_facet | Honghai Zhang Honghai Zhang Yuan Wang Roman Brunecky Bin Yao Xiangming Xie Fei Zheng Huiying Luo |
| author_sort | Honghai Zhang |
| collection | DOAJ |
| description | Swollenins exist within some fungal species and are candidate accessory proteins for the biodegradation of cellulosic substrates. Here, we describe the identification of a swollenin gene, Tlswo, in Talaromyces leycettanus JCM12802. Tlswo was successfully expressed in both Trichoderma reesei and Pichia pastoris. Assay results indicate that TlSWO is capable of releasing reducing sugars from lichenan, barley β-glucan, carboxymethyl cellulose sodium (CMC-Na) and laminarin. The specific activity of TlSWO toward lichenan, barley β-glucan, carboxymethyl cellulose sodium (CMC-Na) and laminarin is 9.0 ± 0.100, 8.9 ± 0.100, 2.3 ± 0.002 and 0.79 ± 0.002 U/mg, respectively. Additionally, TlSWO had disruptive activity on Avicel and a synergistic effect with cellobiohydrolases, increasing the activity on pretreated corn stover by up to 72.2%. The functional diversity of TlSWO broadens its applicability in experimental settings, and indicating that it may be a promising candidate for future industrial applications. |
| first_indexed | 2024-12-14T15:20:05Z |
| format | Article |
| id | doaj.art-a4e2e77027a842fc99ddf81f38b24421 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T15:20:05Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-a4e2e77027a842fc99ddf81f38b244212022-12-21T22:56:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-03-011210.3389/fmicb.2021.658096658096A Swollenin From Talaromyces leycettanus JCM12802 Enhances Cellulase Hydrolysis Toward Various SubstratesHonghai Zhang0Honghai Zhang1Yuan Wang2Roman Brunecky3Bin Yao4Xiangming Xie5Fei Zheng6Huiying Luo7College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaBiosciences Center, National Renewable Energy Laboratory, Golden, CO, United StatesInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaSwollenins exist within some fungal species and are candidate accessory proteins for the biodegradation of cellulosic substrates. Here, we describe the identification of a swollenin gene, Tlswo, in Talaromyces leycettanus JCM12802. Tlswo was successfully expressed in both Trichoderma reesei and Pichia pastoris. Assay results indicate that TlSWO is capable of releasing reducing sugars from lichenan, barley β-glucan, carboxymethyl cellulose sodium (CMC-Na) and laminarin. The specific activity of TlSWO toward lichenan, barley β-glucan, carboxymethyl cellulose sodium (CMC-Na) and laminarin is 9.0 ± 0.100, 8.9 ± 0.100, 2.3 ± 0.002 and 0.79 ± 0.002 U/mg, respectively. Additionally, TlSWO had disruptive activity on Avicel and a synergistic effect with cellobiohydrolases, increasing the activity on pretreated corn stover by up to 72.2%. The functional diversity of TlSWO broadens its applicability in experimental settings, and indicating that it may be a promising candidate for future industrial applications.https://www.frontiersin.org/articles/10.3389/fmicb.2021.658096/fulldisruptive activitysynergistic effecthydrolyticbiomass conversionswollenin |
| spellingShingle | Honghai Zhang Honghai Zhang Yuan Wang Roman Brunecky Bin Yao Xiangming Xie Fei Zheng Huiying Luo A Swollenin From Talaromyces leycettanus JCM12802 Enhances Cellulase Hydrolysis Toward Various Substrates Frontiers in Microbiology disruptive activity synergistic effect hydrolytic biomass conversion swollenin |
| title | A Swollenin From Talaromyces leycettanus JCM12802 Enhances Cellulase Hydrolysis Toward Various Substrates |
| title_full | A Swollenin From Talaromyces leycettanus JCM12802 Enhances Cellulase Hydrolysis Toward Various Substrates |
| title_fullStr | A Swollenin From Talaromyces leycettanus JCM12802 Enhances Cellulase Hydrolysis Toward Various Substrates |
| title_full_unstemmed | A Swollenin From Talaromyces leycettanus JCM12802 Enhances Cellulase Hydrolysis Toward Various Substrates |
| title_short | A Swollenin From Talaromyces leycettanus JCM12802 Enhances Cellulase Hydrolysis Toward Various Substrates |
| title_sort | swollenin from talaromyces leycettanus jcm12802 enhances cellulase hydrolysis toward various substrates |
| topic | disruptive activity synergistic effect hydrolytic biomass conversion swollenin |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.658096/full |
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