Identification, Characterization, and Genomic Analysis of Novel <i>Serratia</i> Temperate Phages from a Gold Mine
Bacteria of the genus <i>Serratia</i> inhabit a variety of ecological niches like water, soil, and the bodies of animals, and have a wide range of lifestyles. Currently, the complete genome sequences of 25 <i>Serratia</i> phages are available in the NCBI database. All of them...
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MDPI AG
2020-09-01
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| Series: | International Journal of Molecular Sciences |
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| author | Katarzyna Bujak Przemyslaw Decewicz Jerzy Kaminski Monika Radlinska |
| author_facet | Katarzyna Bujak Przemyslaw Decewicz Jerzy Kaminski Monika Radlinska |
| author_sort | Katarzyna Bujak |
| collection | DOAJ |
| description | Bacteria of the genus <i>Serratia</i> inhabit a variety of ecological niches like water, soil, and the bodies of animals, and have a wide range of lifestyles. Currently, the complete genome sequences of 25 <i>Serratia</i> phages are available in the NCBI database. All of them were isolated from nutrient-rich environments like sewage, with the use of clinical <i>Serratia</i> strains as hosts. In this study, we identified a novel <i>Serratia</i> myovirus named vB_SspM_BZS1. Both the phage and its host <i>Serratia</i> sp. OS31 were isolated from the same oligotrophic environment, namely, an abandoned gold mine (Zloty Stok, Poland). The BZS1 phage was thoroughly characterized here in terms of its genomics, morphology, and infection kinetics. We also demonstrated that <i>Serratia</i> sp. OS31 was lysogenized by mitomycin-inducible siphovirus vB_SspS_OS31. Comparative analyses revealed that vB_SspM_BZS1 and vB_SspS_OS31 were remote from the known <i>Serratia</i> phages. Moreover, vB_SspM_BZS1 was only distantly related to other viruses. However, we discovered similar prophage sequences in genomes of various bacteria here. Additionally, a protein-based similarity network showed a high diversity of <i>Serratia</i> phages in general, as they were scattered across nineteen different clusters. In summary, this work broadened our knowledge on the diverse relationships of <i>Serratia</i> phages. |
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| language | English |
| last_indexed | 2024-03-10T16:21:44Z |
| publishDate | 2020-09-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a4e54c6cbb1f4dd0b98e8be322aacca62023-11-20T13:36:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-012118670910.3390/ijms21186709Identification, Characterization, and Genomic Analysis of Novel <i>Serratia</i> Temperate Phages from a Gold MineKatarzyna Bujak0Przemyslaw Decewicz1Jerzy Kaminski2Monika Radlinska3Department of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandDepartment of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandDepartment of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandDepartment of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandBacteria of the genus <i>Serratia</i> inhabit a variety of ecological niches like water, soil, and the bodies of animals, and have a wide range of lifestyles. Currently, the complete genome sequences of 25 <i>Serratia</i> phages are available in the NCBI database. All of them were isolated from nutrient-rich environments like sewage, with the use of clinical <i>Serratia</i> strains as hosts. In this study, we identified a novel <i>Serratia</i> myovirus named vB_SspM_BZS1. Both the phage and its host <i>Serratia</i> sp. OS31 were isolated from the same oligotrophic environment, namely, an abandoned gold mine (Zloty Stok, Poland). The BZS1 phage was thoroughly characterized here in terms of its genomics, morphology, and infection kinetics. We also demonstrated that <i>Serratia</i> sp. OS31 was lysogenized by mitomycin-inducible siphovirus vB_SspS_OS31. Comparative analyses revealed that vB_SspM_BZS1 and vB_SspS_OS31 were remote from the known <i>Serratia</i> phages. Moreover, vB_SspM_BZS1 was only distantly related to other viruses. However, we discovered similar prophage sequences in genomes of various bacteria here. Additionally, a protein-based similarity network showed a high diversity of <i>Serratia</i> phages in general, as they were scattered across nineteen different clusters. In summary, this work broadened our knowledge on the diverse relationships of <i>Serratia</i> phages.https://www.mdpi.com/1422-0067/21/18/6709bacteriophageprophagetemperate viruscomparative genomics<i>Serratia</i>dam-like methyltransferase |
| spellingShingle | Katarzyna Bujak Przemyslaw Decewicz Jerzy Kaminski Monika Radlinska Identification, Characterization, and Genomic Analysis of Novel <i>Serratia</i> Temperate Phages from a Gold Mine International Journal of Molecular Sciences bacteriophage prophage temperate virus comparative genomics <i>Serratia</i> dam-like methyltransferase |
| title | Identification, Characterization, and Genomic Analysis of Novel <i>Serratia</i> Temperate Phages from a Gold Mine |
| title_full | Identification, Characterization, and Genomic Analysis of Novel <i>Serratia</i> Temperate Phages from a Gold Mine |
| title_fullStr | Identification, Characterization, and Genomic Analysis of Novel <i>Serratia</i> Temperate Phages from a Gold Mine |
| title_full_unstemmed | Identification, Characterization, and Genomic Analysis of Novel <i>Serratia</i> Temperate Phages from a Gold Mine |
| title_short | Identification, Characterization, and Genomic Analysis of Novel <i>Serratia</i> Temperate Phages from a Gold Mine |
| title_sort | identification characterization and genomic analysis of novel i serratia i temperate phages from a gold mine |
| topic | bacteriophage prophage temperate virus comparative genomics <i>Serratia</i> dam-like methyltransferase |
| url | https://www.mdpi.com/1422-0067/21/18/6709 |
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