New Advances in Dye Analyses: In Situ Gel-Supported Liquid Extraction from Paint Layers and Textiles for SERS and HPLC-MS/MS Identification
To date, it is still not possible to obtain exhaustive information about organic materials in cultural heritage without sampling. Nonetheless, when studying unique objects with invaluable artistic or historical significance, preserving their integrity is a priority. In particular, organic dye identi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/14/5290 |
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| author | Adele Bosi Greta Peruzzi Alessandro Ciccola Ilaria Serafini Flaminia Vincenti Camilla Montesano Paolo Postorino Manuel Sergi Gabriele Favero Roberta Curini |
| author_facet | Adele Bosi Greta Peruzzi Alessandro Ciccola Ilaria Serafini Flaminia Vincenti Camilla Montesano Paolo Postorino Manuel Sergi Gabriele Favero Roberta Curini |
| author_sort | Adele Bosi |
| collection | DOAJ |
| description | To date, it is still not possible to obtain exhaustive information about organic materials in cultural heritage without sampling. Nonetheless, when studying unique objects with invaluable artistic or historical significance, preserving their integrity is a priority. In particular, organic dye identification is of significant interest for history and conservation research, but it is still hindered by analytes’ low concentration and poor fastness. In this work, a minimally invasive approach for dye identification is presented. The procedure is designed to accompany noninvasive analyses of inorganic substances for comprehensive studies of complex cultural heritage matrices, in compliance with their soundness. Liquid extraction of madder, turmeric, and indigo dyes was performed directly from paint layers and textiles. The extraction was supported by hydrogels, which themselves can undergo multitechnique analyses in the place of samples. After extraction, Ag colloid pastes were applied on the gels for SERS analyses, allowing for the identification of the three dyes. For the HPLC-MS/MS analyses, re-extraction of the dyes was followed by a clean-up step that was successfully applied on madder and turmeric. The colour change perceptivity after extraction was measured with colorimetry. The results showed ΔE values mostly below the upper limit of rigorous colour change, confirming the gentleness of the procedure. |
| first_indexed | 2024-03-11T00:48:07Z |
| format | Article |
| id | doaj.art-a4e9cb5616534d4ea1cd0133f933bcc9 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T00:48:07Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a4e9cb5616534d4ea1cd0133f933bcc92023-11-18T20:39:57ZengMDPI AGMolecules1420-30492023-07-012814529010.3390/molecules28145290New Advances in Dye Analyses: In Situ Gel-Supported Liquid Extraction from Paint Layers and Textiles for SERS and HPLC-MS/MS IdentificationAdele Bosi0Greta Peruzzi1Alessandro Ciccola2Ilaria Serafini3Flaminia Vincenti4Camilla Montesano5Paolo Postorino6Manuel Sergi7Gabriele Favero8Roberta Curini9Department of Chemistry, Sapienza University of Rome, P. le Aldo Moro 5, 00185 Rome, ItalyInstitute for Complex System, National Research Council, Sapienza University, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, P. le Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, P. le Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, P. le Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, P. le Aldo Moro 5, 00185 Rome, ItalyDepartment of Physics, Sapienza University of Rome, P. le Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, P. le Aldo Moro 5, 00185 Rome, ItalyDepartment of Environmental Biology, Sapienza University of Rome, P. le Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, P. le Aldo Moro 5, 00185 Rome, ItalyTo date, it is still not possible to obtain exhaustive information about organic materials in cultural heritage without sampling. Nonetheless, when studying unique objects with invaluable artistic or historical significance, preserving their integrity is a priority. In particular, organic dye identification is of significant interest for history and conservation research, but it is still hindered by analytes’ low concentration and poor fastness. In this work, a minimally invasive approach for dye identification is presented. The procedure is designed to accompany noninvasive analyses of inorganic substances for comprehensive studies of complex cultural heritage matrices, in compliance with their soundness. Liquid extraction of madder, turmeric, and indigo dyes was performed directly from paint layers and textiles. The extraction was supported by hydrogels, which themselves can undergo multitechnique analyses in the place of samples. After extraction, Ag colloid pastes were applied on the gels for SERS analyses, allowing for the identification of the three dyes. For the HPLC-MS/MS analyses, re-extraction of the dyes was followed by a clean-up step that was successfully applied on madder and turmeric. The colour change perceptivity after extraction was measured with colorimetry. The results showed ΔE values mostly below the upper limit of rigorous colour change, confirming the gentleness of the procedure.https://www.mdpi.com/1420-3049/28/14/5290dyesin situ extractionhydrogelsSERSdLLMEHPLC-MS/MS |
| spellingShingle | Adele Bosi Greta Peruzzi Alessandro Ciccola Ilaria Serafini Flaminia Vincenti Camilla Montesano Paolo Postorino Manuel Sergi Gabriele Favero Roberta Curini New Advances in Dye Analyses: In Situ Gel-Supported Liquid Extraction from Paint Layers and Textiles for SERS and HPLC-MS/MS Identification Molecules dyes in situ extraction hydrogels SERS dLLME HPLC-MS/MS |
| title | New Advances in Dye Analyses: In Situ Gel-Supported Liquid Extraction from Paint Layers and Textiles for SERS and HPLC-MS/MS Identification |
| title_full | New Advances in Dye Analyses: In Situ Gel-Supported Liquid Extraction from Paint Layers and Textiles for SERS and HPLC-MS/MS Identification |
| title_fullStr | New Advances in Dye Analyses: In Situ Gel-Supported Liquid Extraction from Paint Layers and Textiles for SERS and HPLC-MS/MS Identification |
| title_full_unstemmed | New Advances in Dye Analyses: In Situ Gel-Supported Liquid Extraction from Paint Layers and Textiles for SERS and HPLC-MS/MS Identification |
| title_short | New Advances in Dye Analyses: In Situ Gel-Supported Liquid Extraction from Paint Layers and Textiles for SERS and HPLC-MS/MS Identification |
| title_sort | new advances in dye analyses in situ gel supported liquid extraction from paint layers and textiles for sers and hplc ms ms identification |
| topic | dyes in situ extraction hydrogels SERS dLLME HPLC-MS/MS |
| url | https://www.mdpi.com/1420-3049/28/14/5290 |
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