Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART)
A cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with o...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SciELO
2008-01-01
|
| Series: | Journal of Venomous Animals and Toxins including Tropical Diseases |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400011 |
| _version_ | 1818827533392543744 |
|---|---|
| author | DA Meira RAMB Almeida AN Barbosa LR de Souza TET Olivo RMS Henriques MA Golim JP Araújo Jr LR Nagoshi CM Orikaza SA Calvi |
| author_facet | DA Meira RAMB Almeida AN Barbosa LR de Souza TET Olivo RMS Henriques MA Golim JP Araújo Jr LR Nagoshi CM Orikaza SA Calvi |
| author_sort | DA Meira |
| collection | DOAJ |
| description | A cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G1 = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G2 = 27); and patients on HAART with undetectable VL for at least the past six months (G3 = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G4 = 20), which was the control group. Serum cytokine levels (values in pg/mL) were measured by enzyme-linked immunosorbent assay (ELISA) and specific mRNA expression by reverse transcription polymerase chain reaction (RT-PCR). Both techniques were performed on the four groups for TNF-α, IL-2, INF-γ, IL-4 and IL-10. All patients were submitted to VL determination and CD4+ and CD8+T lymphocyte counts. The analysis of the results revealed a significant comparison among groups for both methods and an association between the latter (> 80% r² > 0.80). There was only one exception, in control individuals for IL-2 by ELISA. The cytokine profiles, in both methods, for the three patient groups, were mature Th-0. The behaviors of IL-2 and INF-γ required emphasis due to consequent expression of dominant Th profile. Both methods showed low IL-2 and high mean values of INF-γ in the three groups. Several authors have recently drawn attention to the substantial apoptosis of infected and non-infected CD4+T cells, mainly during primary infection, persisting only in those with INF-γ phenotype producer and not IL-2. HIV infected individuals submitted to HAART are expected to produce IL-2 in an attempt to present Th-1 profile, but in most cases this did not occur. |
| first_indexed | 2024-12-19T00:45:04Z |
| format | Article |
| id | doaj.art-a4ea505474d042ddac11bc78494442ed |
| institution | Directory Open Access Journal |
| issn | 1678-9199 |
| language | English |
| last_indexed | 2024-12-19T00:45:04Z |
| publishDate | 2008-01-01 |
| publisher | SciELO |
| record_format | Article |
| series | Journal of Venomous Animals and Toxins including Tropical Diseases |
| spelling | doaj.art-a4ea505474d042ddac11bc78494442ed2022-12-21T20:44:19ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992008-01-0114468570210.1590/S1678-91992008000400011Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART)DA MeiraRAMB AlmeidaAN BarbosaLR de SouzaTET OlivoRMS HenriquesMA GolimJP Araújo JrLR NagoshiCM OrikazaSA CalviA cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G1 = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G2 = 27); and patients on HAART with undetectable VL for at least the past six months (G3 = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G4 = 20), which was the control group. Serum cytokine levels (values in pg/mL) were measured by enzyme-linked immunosorbent assay (ELISA) and specific mRNA expression by reverse transcription polymerase chain reaction (RT-PCR). Both techniques were performed on the four groups for TNF-α, IL-2, INF-γ, IL-4 and IL-10. All patients were submitted to VL determination and CD4+ and CD8+T lymphocyte counts. The analysis of the results revealed a significant comparison among groups for both methods and an association between the latter (> 80% r² > 0.80). There was only one exception, in control individuals for IL-2 by ELISA. The cytokine profiles, in both methods, for the three patient groups, were mature Th-0. The behaviors of IL-2 and INF-γ required emphasis due to consequent expression of dominant Th profile. Both methods showed low IL-2 and high mean values of INF-γ in the three groups. Several authors have recently drawn attention to the substantial apoptosis of infected and non-infected CD4+T cells, mainly during primary infection, persisting only in those with INF-γ phenotype producer and not IL-2. HIV infected individuals submitted to HAART are expected to produce IL-2 in an attempt to present Th-1 profile, but in most cases this did not occur.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400011RT-PCRELISAcytokinesHIVAIDSHAARTapoptosis |
| spellingShingle | DA Meira RAMB Almeida AN Barbosa LR de Souza TET Olivo RMS Henriques MA Golim JP Araújo Jr LR Nagoshi CM Orikaza SA Calvi Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART) Journal of Venomous Animals and Toxins including Tropical Diseases RT-PCR ELISA cytokines HIV AIDS HAART apoptosis |
| title | Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART) |
| title_full | Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART) |
| title_fullStr | Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART) |
| title_full_unstemmed | Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART) |
| title_short | Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART) |
| title_sort | assessment of cytokine values in serum by rt pcr in hiv 1 infected individuals with and without highly active anti retroviral therapy haart |
| topic | RT-PCR ELISA cytokines HIV AIDS HAART apoptosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400011 |
| work_keys_str_mv | AT dameira assessmentofcytokinevaluesinserumbyrtpcrinhiv1infectedindividualswithandwithouthighlyactiveantiretroviraltherapyhaart AT rambalmeida assessmentofcytokinevaluesinserumbyrtpcrinhiv1infectedindividualswithandwithouthighlyactiveantiretroviraltherapyhaart AT anbarbosa assessmentofcytokinevaluesinserumbyrtpcrinhiv1infectedindividualswithandwithouthighlyactiveantiretroviraltherapyhaart AT lrdesouza assessmentofcytokinevaluesinserumbyrtpcrinhiv1infectedindividualswithandwithouthighlyactiveantiretroviraltherapyhaart AT tetolivo assessmentofcytokinevaluesinserumbyrtpcrinhiv1infectedindividualswithandwithouthighlyactiveantiretroviraltherapyhaart AT rmshenriques assessmentofcytokinevaluesinserumbyrtpcrinhiv1infectedindividualswithandwithouthighlyactiveantiretroviraltherapyhaart AT magolim assessmentofcytokinevaluesinserumbyrtpcrinhiv1infectedindividualswithandwithouthighlyactiveantiretroviraltherapyhaart AT jparaujojr assessmentofcytokinevaluesinserumbyrtpcrinhiv1infectedindividualswithandwithouthighlyactiveantiretroviraltherapyhaart AT lrnagoshi assessmentofcytokinevaluesinserumbyrtpcrinhiv1infectedindividualswithandwithouthighlyactiveantiretroviraltherapyhaart AT cmorikaza assessmentofcytokinevaluesinserumbyrtpcrinhiv1infectedindividualswithandwithouthighlyactiveantiretroviraltherapyhaart AT sacalvi assessmentofcytokinevaluesinserumbyrtpcrinhiv1infectedindividualswithandwithouthighlyactiveantiretroviraltherapyhaart |