Metallothioneins in Dental Implant Treatment Failure and Periodontitis in Patients with Down’s Syndrome: Validation of Results
Metallothioneins (MTs) are low molecular weight cysteine-rich proteins that can bind up to seven zinc ions. Among their numerous functions, MTs appear to act as protectors against oxidative and inflammatory injury. In our first published study, we reported downregulation of the isoforms <i>MT1...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/6/1028 |
| _version_ | 1797487128517541888 |
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| author | María Baus-Domínguez Raquel Gómez-Díaz José-Luis Gutiérrez-Pérez Daniel Torres-Lagares Guillermo Machuca-Portillo María-Ángeles Serrera-Figallo |
| author_facet | María Baus-Domínguez Raquel Gómez-Díaz José-Luis Gutiérrez-Pérez Daniel Torres-Lagares Guillermo Machuca-Portillo María-Ángeles Serrera-Figallo |
| author_sort | María Baus-Domínguez |
| collection | DOAJ |
| description | Metallothioneins (MTs) are low molecular weight cysteine-rich proteins that can bind up to seven zinc ions. Among their numerous functions, MTs appear to act as protectors against oxidative and inflammatory injury. In our first published study, we reported downregulation of the isoforms <i>MT1B</i> (fold distance (FD) −2. 95; <i>p</i> = 0.0024), <i>MT1F</i> (FD −1.72; <i>p</i> = 0.0276), <i>MT1X</i> (FD −3.09; <i>p</i> = 0.0021), <i>MT1H</i> (FD −2.39; <i>p</i> = 0.0018), <i>MT1M</i> (FD −2.37; <i>p</i> = 0.0092), <i>MT1L</i> (FD −2. 55; <i>p</i> = 0.0048), <i>MT1E</i> (FD −2.71; <i>p</i> = 0.0014), <i>MT2A</i> (FD −2.35; <i>p</i> = 0.0072), <i>MT1G</i> (FD −2.24; <i>p</i> = 0.0118), and <i>MT1A</i> (FD −2.82; <i>p</i> = 0.0023) by comparing Down’s syndrome patients with periodontal disease and implant failure to those without periodontal disease and with a positive progression of their implants. In this gene validation study, we intended to verify the results of our first gene expression analysis. Materials and Methods: In our retrospective case–control study, we performed retrotranscription (RT-qPCR) of 11 RNA-to-cDNA samples using the SuperScript™ VILO™ kit (50; reference 1,176,605) from Thermo Fisher. We conducted the study using the real-time PCR technique on the q-PCR ViiA 7 platform from Thermo Fisher. We chose the format of the Taqman Array Plate 16 Plus (reference 4,413,261) from Thermo Fisher, which accommodates 12 genes plus four controls (<i>GAPDH, 18S, ACTB,</i> and <i>HPRT1</i>). We conducted the analysis of the plates using the Thermo Fisher Cloud Web Software. Results: The results obtained through gene validation analysis show that in PD+RI+ patients, the genes encoding the isoforms <i>MT1F</i> (FD 0.3; <i>p</i> = 0.039), <i>MT1X</i> (FD 338; <i>p</i> = 0.0078), <i>MT1E</i> (FD 307; <i>p</i> = 0.0358), and <i>MT2A</i> (FD 252; <i>p</i> = 0.0428) continue to show downregulation, whereas <i>MT1B</i> (FD 2.75; <i>p</i> = 0.580), <i>MT1H</i> (FD 281; <i>p</i> = 0.152), <i>MT1L</i> (FD 354; <i>p</i> = 0.0965), and <i>MT1G</i> (FD 336; <i>p</i> = 0.0749) no longer show statistically significant results. |
| first_indexed | 2024-03-09T23:44:02Z |
| format | Article |
| id | doaj.art-a4f12fd816dd42a1a16fefc1d447605d |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T23:44:02Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-a4f12fd816dd42a1a16fefc1d447605d2023-11-23T16:48:06ZengMDPI AGGenes2073-44252022-06-01136102810.3390/genes13061028Metallothioneins in Dental Implant Treatment Failure and Periodontitis in Patients with Down’s Syndrome: Validation of ResultsMaría Baus-Domínguez0Raquel Gómez-Díaz1José-Luis Gutiérrez-Pérez2Daniel Torres-Lagares3Guillermo Machuca-Portillo4María-Ángeles Serrera-Figallo5Department of Dentistry, Faculty of Dentistry, University of Seville, 41009 Seville, SpainInstitute of Biomedicine of Seville, 41013 Seville, SpainOral and Maxillofacial Unit, Virgen del Rocio Hospital, 41013 Seville, SpainDepartment of Dentistry, Faculty of Dentistry, University of Seville, 41009 Seville, SpainDepartment of Dentistry, Faculty of Dentistry, University of Seville, 41009 Seville, SpainDepartment of Dentistry, Faculty of Dentistry, University of Seville, 41009 Seville, SpainMetallothioneins (MTs) are low molecular weight cysteine-rich proteins that can bind up to seven zinc ions. Among their numerous functions, MTs appear to act as protectors against oxidative and inflammatory injury. In our first published study, we reported downregulation of the isoforms <i>MT1B</i> (fold distance (FD) −2. 95; <i>p</i> = 0.0024), <i>MT1F</i> (FD −1.72; <i>p</i> = 0.0276), <i>MT1X</i> (FD −3.09; <i>p</i> = 0.0021), <i>MT1H</i> (FD −2.39; <i>p</i> = 0.0018), <i>MT1M</i> (FD −2.37; <i>p</i> = 0.0092), <i>MT1L</i> (FD −2. 55; <i>p</i> = 0.0048), <i>MT1E</i> (FD −2.71; <i>p</i> = 0.0014), <i>MT2A</i> (FD −2.35; <i>p</i> = 0.0072), <i>MT1G</i> (FD −2.24; <i>p</i> = 0.0118), and <i>MT1A</i> (FD −2.82; <i>p</i> = 0.0023) by comparing Down’s syndrome patients with periodontal disease and implant failure to those without periodontal disease and with a positive progression of their implants. In this gene validation study, we intended to verify the results of our first gene expression analysis. Materials and Methods: In our retrospective case–control study, we performed retrotranscription (RT-qPCR) of 11 RNA-to-cDNA samples using the SuperScript™ VILO™ kit (50; reference 1,176,605) from Thermo Fisher. We conducted the study using the real-time PCR technique on the q-PCR ViiA 7 platform from Thermo Fisher. We chose the format of the Taqman Array Plate 16 Plus (reference 4,413,261) from Thermo Fisher, which accommodates 12 genes plus four controls (<i>GAPDH, 18S, ACTB,</i> and <i>HPRT1</i>). We conducted the analysis of the plates using the Thermo Fisher Cloud Web Software. Results: The results obtained through gene validation analysis show that in PD+RI+ patients, the genes encoding the isoforms <i>MT1F</i> (FD 0.3; <i>p</i> = 0.039), <i>MT1X</i> (FD 338; <i>p</i> = 0.0078), <i>MT1E</i> (FD 307; <i>p</i> = 0.0358), and <i>MT2A</i> (FD 252; <i>p</i> = 0.0428) continue to show downregulation, whereas <i>MT1B</i> (FD 2.75; <i>p</i> = 0.580), <i>MT1H</i> (FD 281; <i>p</i> = 0.152), <i>MT1L</i> (FD 354; <i>p</i> = 0.0965), and <i>MT1G</i> (FD 336; <i>p</i> = 0.0749) no longer show statistically significant results.https://www.mdpi.com/2073-4425/13/6/1028Down’s syndromeperiodontal diseasebone biologyclinical outcomesgene expressionvalidation |
| spellingShingle | María Baus-Domínguez Raquel Gómez-Díaz José-Luis Gutiérrez-Pérez Daniel Torres-Lagares Guillermo Machuca-Portillo María-Ángeles Serrera-Figallo Metallothioneins in Dental Implant Treatment Failure and Periodontitis in Patients with Down’s Syndrome: Validation of Results Genes Down’s syndrome periodontal disease bone biology clinical outcomes gene expression validation |
| title | Metallothioneins in Dental Implant Treatment Failure and Periodontitis in Patients with Down’s Syndrome: Validation of Results |
| title_full | Metallothioneins in Dental Implant Treatment Failure and Periodontitis in Patients with Down’s Syndrome: Validation of Results |
| title_fullStr | Metallothioneins in Dental Implant Treatment Failure and Periodontitis in Patients with Down’s Syndrome: Validation of Results |
| title_full_unstemmed | Metallothioneins in Dental Implant Treatment Failure and Periodontitis in Patients with Down’s Syndrome: Validation of Results |
| title_short | Metallothioneins in Dental Implant Treatment Failure and Periodontitis in Patients with Down’s Syndrome: Validation of Results |
| title_sort | metallothioneins in dental implant treatment failure and periodontitis in patients with down s syndrome validation of results |
| topic | Down’s syndrome periodontal disease bone biology clinical outcomes gene expression validation |
| url | https://www.mdpi.com/2073-4425/13/6/1028 |
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