Effect of Probiotics on the Traumatic Brain Injury
Objective: There is a two way relationship between the gut microbiota and the brain. Oxidative stress and inflammation have important role in this damage of traumatic brain (TB). Pathologic alterations of intestine enviroment cause more problem for treatment of brain damage. Probiotics (PB) may pote...
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| Format: | Article |
| Language: | English |
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Galenos Yayinevi
2022-04-01
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| Series: | Forbes Tıp Dergisi |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=forbes&un=FJM-48658 |
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| author | Müge KARAKAYALI Erdoğan KOCAMAZ Şüheda ALPAY Tuna ÖNAL Mustafa ÖZTATLICI Rabia DURUŞMA Hasan Fehmi ÖZEL Mesut METE Necip KUTLU Mehmet İbrahim TUĞLU |
| author_facet | Müge KARAKAYALI Erdoğan KOCAMAZ Şüheda ALPAY Tuna ÖNAL Mustafa ÖZTATLICI Rabia DURUŞMA Hasan Fehmi ÖZEL Mesut METE Necip KUTLU Mehmet İbrahim TUĞLU |
| author_sort | Müge KARAKAYALI |
| collection | DOAJ |
| description | Objective: There is a two way relationship between the gut microbiota and the brain. Oxidative stress and inflammation have important role in this damage of traumatic brain (TB). Pathologic alterations of intestine enviroment cause more problem for treatment of brain damage. Probiotics (PB) may potential to reduce oxidative stress and gliosis. In this study, experimental TB injury (TBI) was provided to examine this possible effect of PB.
Methods: Weight-drop method was used to produce TBI and the effect of daily PB application was examined. For PB application, non-infective NBL Probiotic Gold preparation 2.5x109 Enterococcus faecium, 2.5x109 Lactobacillus acidophilus, 2.5x109 Lactobacillus rhamnosus, 2.5x109 Bifidobacterium longum, 2.5x109 Bifidobacterium bifidum bacteria were administered orally. Oxidative stress was analyzed by endothelial nitric oxide synthase (eNOS), gliosis by glial fibrillary acidic protein (GFAP) and S100 and inflamation by IL-10 with IHC. Damage was assessed by scoring with HE staining and h-score of IHC.
Results: PB caused significant neuron protection and more healing according to HE scoring in TBI. This improvement was in parallel with increasing of h-score IL-10 and decreasing of eNOS, GFAP and S100 which provided better healing.
Conclusion: In this study, damage of TBI was treated with PB which caused decrase of oxidative stress, gliosis and inflamation. By this way, better neuron protection, faster healing and more treatment occured. This could be very important for patient with TBI which is diffucult and cost effective treatment. |
| first_indexed | 2024-03-12T20:40:23Z |
| format | Article |
| id | doaj.art-a4f3b9c4f89f4104ba7cb1c8612e22ea |
| institution | Directory Open Access Journal |
| issn | 2757-5241 |
| language | English |
| last_indexed | 2024-03-12T20:40:23Z |
| publishDate | 2022-04-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Forbes Tıp Dergisi |
| spelling | doaj.art-a4f3b9c4f89f4104ba7cb1c8612e22ea2023-08-01T10:46:54ZengGalenos YayineviForbes Tıp Dergisi2757-52412022-04-0131596710.4274/forbes.galenos.2022.48658FJM-48658Effect of Probiotics on the Traumatic Brain InjuryMüge KARAKAYALI0Erdoğan KOCAMAZ1Şüheda ALPAY2Tuna ÖNAL3Mustafa ÖZTATLICI4Rabia DURUŞMA5Hasan Fehmi ÖZEL6Mesut METE7Necip KUTLU8Mehmet İbrahim TUĞLU9Medical Microbiology Department,Izmir Democracy University School of Medicine, TurkeyManisa Celal Bayar University School of Medicine Histology and Embryology Department/TurkeyManisa Celal Bayar University School of Medicine Physiology Department/Manisa/TurkeyManisa Celal Bayar University School of Medicine Histology and Embryology Department/TurkeyManisa Celal Bayar University School of Medicine Histology and Embryology Department/TurkeyManisa Celal Bayar University School of Medicine Histology and Embryology Department/TurkeyManisa Celal Bayar University School of Medicine Physiology Department/Manisa/TurkeyCelal Bayar University School of Medicine Neurosurgery Department /TurkeyManisa Celal Bayar University School of Medicine Physiology Department/Manisa/TurkeyManisa Celal Bayar University School of Medicine Histology and Embryology Department/TurkeyObjective: There is a two way relationship between the gut microbiota and the brain. Oxidative stress and inflammation have important role in this damage of traumatic brain (TB). Pathologic alterations of intestine enviroment cause more problem for treatment of brain damage. Probiotics (PB) may potential to reduce oxidative stress and gliosis. In this study, experimental TB injury (TBI) was provided to examine this possible effect of PB. Methods: Weight-drop method was used to produce TBI and the effect of daily PB application was examined. For PB application, non-infective NBL Probiotic Gold preparation 2.5x109 Enterococcus faecium, 2.5x109 Lactobacillus acidophilus, 2.5x109 Lactobacillus rhamnosus, 2.5x109 Bifidobacterium longum, 2.5x109 Bifidobacterium bifidum bacteria were administered orally. Oxidative stress was analyzed by endothelial nitric oxide synthase (eNOS), gliosis by glial fibrillary acidic protein (GFAP) and S100 and inflamation by IL-10 with IHC. Damage was assessed by scoring with HE staining and h-score of IHC. Results: PB caused significant neuron protection and more healing according to HE scoring in TBI. This improvement was in parallel with increasing of h-score IL-10 and decreasing of eNOS, GFAP and S100 which provided better healing. Conclusion: In this study, damage of TBI was treated with PB which caused decrase of oxidative stress, gliosis and inflamation. By this way, better neuron protection, faster healing and more treatment occured. This could be very important for patient with TBI which is diffucult and cost effective treatment.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=forbes&un=FJM-48658traumatic brain injuryprobioticoxidative stressinflammationgliosis |
| spellingShingle | Müge KARAKAYALI Erdoğan KOCAMAZ Şüheda ALPAY Tuna ÖNAL Mustafa ÖZTATLICI Rabia DURUŞMA Hasan Fehmi ÖZEL Mesut METE Necip KUTLU Mehmet İbrahim TUĞLU Effect of Probiotics on the Traumatic Brain Injury Forbes Tıp Dergisi traumatic brain injury probiotic oxidative stress inflammation gliosis |
| title | Effect of Probiotics on the Traumatic Brain Injury |
| title_full | Effect of Probiotics on the Traumatic Brain Injury |
| title_fullStr | Effect of Probiotics on the Traumatic Brain Injury |
| title_full_unstemmed | Effect of Probiotics on the Traumatic Brain Injury |
| title_short | Effect of Probiotics on the Traumatic Brain Injury |
| title_sort | effect of probiotics on the traumatic brain injury |
| topic | traumatic brain injury probiotic oxidative stress inflammation gliosis |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=forbes&un=FJM-48658 |
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