De Novo Transcriptomic Analyses Revealed Some Detoxification Genes and Related Pathways Responsive to Noposion Yihaogong<sup>®</sup> 5% EC (Lambda-Cyhalothrin 5%) Exposure in <i>Spodoptera frugiperda</i> Third-Instar Larvae
The fall armyworm, <i>Spodoptera frugiperda</i> (J.E. Smith) (Lepidoptera: Noctuidae), is a polyphagous, invasive insect pest which causes significant losses in important crops wherever it has spread. The use of pesticides in agriculture is a key tool in the management of many important...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/2/132 |
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| author | Muhammad Hafeez Xiaowei Li Zhijun Zhang Jun Huang Likun Wang Jinming Zhang Sakhawat Shah Muhammad Musa Khan Fei Xu G. Mandela Fernández-Grandon Myron P. Zalucki Yaobin Lu |
| author_facet | Muhammad Hafeez Xiaowei Li Zhijun Zhang Jun Huang Likun Wang Jinming Zhang Sakhawat Shah Muhammad Musa Khan Fei Xu G. Mandela Fernández-Grandon Myron P. Zalucki Yaobin Lu |
| author_sort | Muhammad Hafeez |
| collection | DOAJ |
| description | The fall armyworm, <i>Spodoptera frugiperda</i> (J.E. Smith) (Lepidoptera: Noctuidae), is a polyphagous, invasive insect pest which causes significant losses in important crops wherever it has spread. The use of pesticides in agriculture is a key tool in the management of many important crop pests, including <i>S. frugiperda</i>, but continued use of insecticides has selected for various types of resistance, including enzyme systems that provide enhanced mechanisms of detoxification. In the present study, we analyzed the de novo transcriptome of <i>S. frugiperda</i> larvae exposed to Noposion Yihaogong<sup>®</sup> 5% emulsifiable concentrate (EC) insecticide focusing on detoxification genes and related pathways. Results showed that a total of 1819 differentially expressed genes (DEGs) were identified in larvae after being treated with Noposion Yihaogong<sup>®</sup> 5% EC insecticide, of which 863 were up- and 956 down-regulated. Majority of these differentially expressed genes were identified in numerous Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including metabolism of xenobiotics and drug metabolism. Furthermore, many of <i>S. frugiperda</i> genes involved in detoxification pathways influenced by lambda-cyhalothrin stress support their predicted role by further co-expression network analysis. Our RT-qPCR results were consistent with the DEG’s data of transcriptome analysis. The comprehensive transcriptome sequence resource attained through this study enriches the genomic platform of <i>S. frugiperda</i>, and the identified DEGs may enable greater molecular underpinnings behind the insecticide-resistance mechanism caused by lambda-cyhalothrin. |
| first_indexed | 2024-03-09T05:56:29Z |
| format | Article |
| id | doaj.art-a4f66bc34416429aad3f20b6fcd8326e |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T05:56:29Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-a4f66bc34416429aad3f20b6fcd8326e2023-12-03T12:13:24ZengMDPI AGInsects2075-44502021-02-0112213210.3390/insects12020132De Novo Transcriptomic Analyses Revealed Some Detoxification Genes and Related Pathways Responsive to Noposion Yihaogong<sup>®</sup> 5% EC (Lambda-Cyhalothrin 5%) Exposure in <i>Spodoptera frugiperda</i> Third-Instar LarvaeMuhammad Hafeez0Xiaowei Li1Zhijun Zhang2Jun Huang3Likun Wang4Jinming Zhang5Sakhawat Shah6Muhammad Musa Khan7Fei Xu8G. Mandela Fernández-Grandon9Myron P. Zalucki10Yaobin Lu11State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaHubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Bio-Pesticide Innovation and Application, South China Agricultural University, Guangzhou 510642, ChinaCentral Laboratory of Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaNatural Resources Institute, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UKSchool of Biological Sciences, The University of Queensland, Brisbane, QLD 4072, AustraliaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaThe fall armyworm, <i>Spodoptera frugiperda</i> (J.E. Smith) (Lepidoptera: Noctuidae), is a polyphagous, invasive insect pest which causes significant losses in important crops wherever it has spread. The use of pesticides in agriculture is a key tool in the management of many important crop pests, including <i>S. frugiperda</i>, but continued use of insecticides has selected for various types of resistance, including enzyme systems that provide enhanced mechanisms of detoxification. In the present study, we analyzed the de novo transcriptome of <i>S. frugiperda</i> larvae exposed to Noposion Yihaogong<sup>®</sup> 5% emulsifiable concentrate (EC) insecticide focusing on detoxification genes and related pathways. Results showed that a total of 1819 differentially expressed genes (DEGs) were identified in larvae after being treated with Noposion Yihaogong<sup>®</sup> 5% EC insecticide, of which 863 were up- and 956 down-regulated. Majority of these differentially expressed genes were identified in numerous Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including metabolism of xenobiotics and drug metabolism. Furthermore, many of <i>S. frugiperda</i> genes involved in detoxification pathways influenced by lambda-cyhalothrin stress support their predicted role by further co-expression network analysis. Our RT-qPCR results were consistent with the DEG’s data of transcriptome analysis. The comprehensive transcriptome sequence resource attained through this study enriches the genomic platform of <i>S. frugiperda</i>, and the identified DEGs may enable greater molecular underpinnings behind the insecticide-resistance mechanism caused by lambda-cyhalothrin.https://www.mdpi.com/2075-4450/12/2/132transcriptome analysis<i>S. frugiperda</i>Noposion Yihaogong<sup>®</sup> 5% EClambda-cyhalothrindetoxification genespathways |
| spellingShingle | Muhammad Hafeez Xiaowei Li Zhijun Zhang Jun Huang Likun Wang Jinming Zhang Sakhawat Shah Muhammad Musa Khan Fei Xu G. Mandela Fernández-Grandon Myron P. Zalucki Yaobin Lu De Novo Transcriptomic Analyses Revealed Some Detoxification Genes and Related Pathways Responsive to Noposion Yihaogong<sup>®</sup> 5% EC (Lambda-Cyhalothrin 5%) Exposure in <i>Spodoptera frugiperda</i> Third-Instar Larvae Insects transcriptome analysis <i>S. frugiperda</i> Noposion Yihaogong<sup>®</sup> 5% EC lambda-cyhalothrin detoxification genes pathways |
| title | De Novo Transcriptomic Analyses Revealed Some Detoxification Genes and Related Pathways Responsive to Noposion Yihaogong<sup>®</sup> 5% EC (Lambda-Cyhalothrin 5%) Exposure in <i>Spodoptera frugiperda</i> Third-Instar Larvae |
| title_full | De Novo Transcriptomic Analyses Revealed Some Detoxification Genes and Related Pathways Responsive to Noposion Yihaogong<sup>®</sup> 5% EC (Lambda-Cyhalothrin 5%) Exposure in <i>Spodoptera frugiperda</i> Third-Instar Larvae |
| title_fullStr | De Novo Transcriptomic Analyses Revealed Some Detoxification Genes and Related Pathways Responsive to Noposion Yihaogong<sup>®</sup> 5% EC (Lambda-Cyhalothrin 5%) Exposure in <i>Spodoptera frugiperda</i> Third-Instar Larvae |
| title_full_unstemmed | De Novo Transcriptomic Analyses Revealed Some Detoxification Genes and Related Pathways Responsive to Noposion Yihaogong<sup>®</sup> 5% EC (Lambda-Cyhalothrin 5%) Exposure in <i>Spodoptera frugiperda</i> Third-Instar Larvae |
| title_short | De Novo Transcriptomic Analyses Revealed Some Detoxification Genes and Related Pathways Responsive to Noposion Yihaogong<sup>®</sup> 5% EC (Lambda-Cyhalothrin 5%) Exposure in <i>Spodoptera frugiperda</i> Third-Instar Larvae |
| title_sort | de novo transcriptomic analyses revealed some detoxification genes and related pathways responsive to noposion yihaogong sup r sup 5 ec lambda cyhalothrin 5 exposure in i spodoptera frugiperda i third instar larvae |
| topic | transcriptome analysis <i>S. frugiperda</i> Noposion Yihaogong<sup>®</sup> 5% EC lambda-cyhalothrin detoxification genes pathways |
| url | https://www.mdpi.com/2075-4450/12/2/132 |
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