Anti-cancer Effects of Methotrexate in Combination With Melissa officinalis on HeLa Cancer Cell Line
Objectives: One of the well-accepted beliefs about natural products, considering the advances of recently appearing new edges and features of herbal medicine, is paying more attention to cancer treatments. However, they have not been properly studied with reasonable/reliable clinical trials in human...
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| Format: | Article |
| Language: | English |
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Aras Part Medical International Press
2022-10-01
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| Series: | Crescent Journal of Medical and Biological Sciences |
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| Online Access: | http://www.cjmb.org/pdf.php?id=556 |
| _version_ | 1797840359628210176 |
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| author | Parviz Faraji Mostafa Araj-Khodaei Amir Jafari Maryam Ghaffari Reza Mohammadinasab Sanaz Hamedeyazdan Miguel de la Guardia Jafar Ezzati Nazhad Dolatabadi |
| author_facet | Parviz Faraji Mostafa Araj-Khodaei Amir Jafari Maryam Ghaffari Reza Mohammadinasab Sanaz Hamedeyazdan Miguel de la Guardia Jafar Ezzati Nazhad Dolatabadi |
| author_sort | Parviz Faraji |
| collection | DOAJ |
| description | Objectives: One of the well-accepted beliefs about natural products, considering the advances of recently appearing new edges and features of herbal medicine, is paying more attention to cancer treatments. However, they have not been properly studied with reasonable/reliable clinical trials in human subjects in most cases. Therefore, seeking in vitro effects of herbs like Melissa officinalis (MO) in cancer therapy to identify the involved possible mechanism in conjugation with configurative/morphological aspects of treated cells seems quite necessary. In this study, we evaluated the co-treatment effect of anti-cancer drug methotrexate (MTX) and MO on HeLa cancer cells. Methods: MTT assay was applied to assess the quantitative cytotoxicity effect of both MTX and Mo. Apoptosis assay via flow cytometry was used to determine the amount of apoptotic and necrotic cells. To further investigate the anti-cancer effects, DAPI staining and DNA ladder assays are used qualitatively to detect changes in the nuclei of cells that are a sign of apoptosis occurring and morphological modifications of DNA. Results: MTX and MO mixture showed high cytotoxicity and apoptosis rate compared to untreated cells. Furthermore, the morphological changes of MTX and MO mixture were more evident than that of single MO, MTX, and control groups. Conclusions: These data regarding cell growth reduction and apoptosis induction in HeLa cancer cells showed that MTX and MO mixture can be an appropriate platform for cancer therapy. |
| first_indexed | 2024-04-09T16:13:46Z |
| format | Article |
| id | doaj.art-a4f793ff1d7145bfb74c9acfca939f6f |
| institution | Directory Open Access Journal |
| issn | 2148-9696 |
| language | English |
| last_indexed | 2024-04-09T16:13:46Z |
| publishDate | 2022-10-01 |
| publisher | Aras Part Medical International Press |
| record_format | Article |
| series | Crescent Journal of Medical and Biological Sciences |
| spelling | doaj.art-a4f793ff1d7145bfb74c9acfca939f6f2023-04-24T07:15:23ZengAras Part Medical International PressCrescent Journal of Medical and Biological Sciences2148-96962022-10-019418919410.34172/cjmb.2022.31CJMB-7078Anti-cancer Effects of Methotrexate in Combination With Melissa officinalis on HeLa Cancer Cell LineParviz Faraji0Mostafa Araj-Khodaei1Amir Jafari2Maryam Ghaffari3Reza Mohammadinasab4Sanaz Hamedeyazdan5Miguel de la Guardia6Jafar Ezzati Nazhad Dolatabadi7Department of Persian Medicine, School of Traditional Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Persian Medicine, School of Traditional Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.Department of History of Medicine, School of Traditional Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain.Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Objectives: One of the well-accepted beliefs about natural products, considering the advances of recently appearing new edges and features of herbal medicine, is paying more attention to cancer treatments. However, they have not been properly studied with reasonable/reliable clinical trials in human subjects in most cases. Therefore, seeking in vitro effects of herbs like Melissa officinalis (MO) in cancer therapy to identify the involved possible mechanism in conjugation with configurative/morphological aspects of treated cells seems quite necessary. In this study, we evaluated the co-treatment effect of anti-cancer drug methotrexate (MTX) and MO on HeLa cancer cells. Methods: MTT assay was applied to assess the quantitative cytotoxicity effect of both MTX and Mo. Apoptosis assay via flow cytometry was used to determine the amount of apoptotic and necrotic cells. To further investigate the anti-cancer effects, DAPI staining and DNA ladder assays are used qualitatively to detect changes in the nuclei of cells that are a sign of apoptosis occurring and morphological modifications of DNA. Results: MTX and MO mixture showed high cytotoxicity and apoptosis rate compared to untreated cells. Furthermore, the morphological changes of MTX and MO mixture were more evident than that of single MO, MTX, and control groups. Conclusions: These data regarding cell growth reduction and apoptosis induction in HeLa cancer cells showed that MTX and MO mixture can be an appropriate platform for cancer therapy.http://www.cjmb.org/pdf.php?id=556methotrexatemelissa officinalishela cancer cellsmtt assayflow cytometry |
| spellingShingle | Parviz Faraji Mostafa Araj-Khodaei Amir Jafari Maryam Ghaffari Reza Mohammadinasab Sanaz Hamedeyazdan Miguel de la Guardia Jafar Ezzati Nazhad Dolatabadi Anti-cancer Effects of Methotrexate in Combination With Melissa officinalis on HeLa Cancer Cell Line Crescent Journal of Medical and Biological Sciences methotrexate melissa officinalis hela cancer cells mtt assay flow cytometry |
| title | Anti-cancer Effects of Methotrexate in Combination With Melissa officinalis on HeLa Cancer Cell Line |
| title_full | Anti-cancer Effects of Methotrexate in Combination With Melissa officinalis on HeLa Cancer Cell Line |
| title_fullStr | Anti-cancer Effects of Methotrexate in Combination With Melissa officinalis on HeLa Cancer Cell Line |
| title_full_unstemmed | Anti-cancer Effects of Methotrexate in Combination With Melissa officinalis on HeLa Cancer Cell Line |
| title_short | Anti-cancer Effects of Methotrexate in Combination With Melissa officinalis on HeLa Cancer Cell Line |
| title_sort | anti cancer effects of methotrexate in combination with melissa officinalis on hela cancer cell line |
| topic | methotrexate melissa officinalis hela cancer cells mtt assay flow cytometry |
| url | http://www.cjmb.org/pdf.php?id=556 |
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