Analysis of the Relationship Between the Degree of Dysbiosis in Gut Microbiota and Prognosis at Different Stages of Primary Hepatocellular Carcinoma
Gut microbiota dysbiosis is closely associated with primary hepatocellular carcinoma (HCC). Recent studies have evaluated the early diagnosis of primary HCC through analysis of gut microbiota dysbiosis. However, the relationship between the degree of dysbiosis and the prognosis of primary HCC remain...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-06-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01458/full |
| _version_ | 1818137003564204032 |
|---|---|
| author | Jiajia Ni Jiajia Ni Rong Huang Huifang Zhou Xiaoping Xu Xiaoping Xu Yang Li Yang Li Peihua Cao Peihua Cao Kebo Zhong Kebo Zhong Mei Ge Mei Ge Xiaoxia Chen Xiaoxia Chen Baohua Hou Min Yu Baogang Peng Qiao Li Peng Zhang Yi Gao Yi Gao |
| author_facet | Jiajia Ni Jiajia Ni Rong Huang Huifang Zhou Xiaoping Xu Xiaoping Xu Yang Li Yang Li Peihua Cao Peihua Cao Kebo Zhong Kebo Zhong Mei Ge Mei Ge Xiaoxia Chen Xiaoxia Chen Baohua Hou Min Yu Baogang Peng Qiao Li Peng Zhang Yi Gao Yi Gao |
| author_sort | Jiajia Ni |
| collection | DOAJ |
| description | Gut microbiota dysbiosis is closely associated with primary hepatocellular carcinoma (HCC). Recent studies have evaluated the early diagnosis of primary HCC through analysis of gut microbiota dysbiosis. However, the relationship between the degree of dysbiosis and the prognosis of primary HCC remains unclear. Because primary HCC is accompanied by dysbiosis and dysbiosis usually increases the circulatory concentrations of endotoxin and other harmful bacterial substances, which further increases liver damage, we hypothesized that level of dysbiosis associated with primary HCC increases with the stage of cancer progression. To test this hypothesis, we introduced a more integrated index referred to as the degree of dysbiosis (Ddys); and we investigated Ddys of the gut microbiota with the development of primary HCC through high-throughput sequencing of 16S rRNA gene amplicons. Our results showed that compared with healthy individuals, patients with primary HCC showed increased pro-inflammatory bacteria in their fecal microbiota. The Ddys increased significantly in patients with primary HCC compared with that in healthy controls. Moreover, there was a tendency for the Ddys to increase with the development of primary HCC, although no significant difference was detected between different stages of primary HCC. Our findings provide important insights into the use of gut microbiota analysis during the treatment of primary HCC. |
| first_indexed | 2024-12-11T09:49:23Z |
| format | Article |
| id | doaj.art-a4f8b07e00214ddd90c25712eb6a7fb2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T09:49:23Z |
| publishDate | 2019-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a4f8b07e00214ddd90c25712eb6a7fb22022-12-22T01:12:28ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-06-011010.3389/fmicb.2019.01458455714Analysis of the Relationship Between the Degree of Dysbiosis in Gut Microbiota and Prognosis at Different Stages of Primary Hepatocellular CarcinomaJiajia Ni0Jiajia Ni1Rong Huang2Huifang Zhou3Xiaoping Xu4Xiaoping Xu5Yang Li6Yang Li7Peihua Cao8Peihua Cao9Kebo Zhong10Kebo Zhong11Mei Ge12Mei Ge13Xiaoxia Chen14Xiaoxia Chen15Baohua Hou16Min Yu17Baogang Peng18Qiao Li19Peng Zhang20Yi Gao21Yi Gao22Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center of Artificial Organ and Tissue Engineering, Zhujiang Hospital of Southern Medical University, Guangzhou, ChinaState Key Laboratory of Organ Failure Research, Southern Medical University, Guangzhou, ChinaDepartment of Neonatal Surgery, Guangdong Women and Children Hospital, Guangzhou, ChinaDepartment of Clinical Laboratory, First People’s Hospital of Kashi, Kashgar, ChinaDepartment of Hepatobiliary Surgery II, Guangdong Provincial Research Center of Artificial Organ and Tissue Engineering, Zhujiang Hospital of Southern Medical University, Guangzhou, ChinaState Key Laboratory of Organ Failure Research, Southern Medical University, Guangzhou, ChinaDepartment of Hepatobiliary Surgery II, Guangdong Provincial Research Center of Artificial Organ and Tissue Engineering, Zhujiang Hospital of Southern Medical University, Guangzhou, ChinaState Key Laboratory of Organ Failure Research, Southern Medical University, Guangzhou, ChinaDepartment of Hepatobiliary Surgery II, Guangdong Provincial Research Center of Artificial Organ and Tissue Engineering, Zhujiang Hospital of Southern Medical University, Guangzhou, ChinaState Key Laboratory of Organ Failure Research, Southern Medical University, Guangzhou, ChinaDepartment of Hepatobiliary Surgery II, Guangdong Provincial Research Center of Artificial Organ and Tissue Engineering, Zhujiang Hospital of Southern Medical University, Guangzhou, ChinaState Key Laboratory of Organ Failure Research, Southern Medical University, Guangzhou, ChinaDepartment of Hepatobiliary Surgery II, Guangdong Provincial Research Center of Artificial Organ and Tissue Engineering, Zhujiang Hospital of Southern Medical University, Guangzhou, ChinaState Key Laboratory of Organ Failure Research, Southern Medical University, Guangzhou, ChinaDepartment of Hepatobiliary Surgery II, Guangdong Provincial Research Center of Artificial Organ and Tissue Engineering, Zhujiang Hospital of Southern Medical University, Guangzhou, ChinaState Key Laboratory of Organ Failure Research, Southern Medical University, Guangzhou, ChinaDepartment of General Surgery, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, ChinaDepartment of General Surgery, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, ChinaDepartment of Hepatic Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Hepatic Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Organ Transplantation, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Hepatobiliary Surgery II, Guangdong Provincial Research Center of Artificial Organ and Tissue Engineering, Zhujiang Hospital of Southern Medical University, Guangzhou, ChinaState Key Laboratory of Organ Failure Research, Southern Medical University, Guangzhou, ChinaGut microbiota dysbiosis is closely associated with primary hepatocellular carcinoma (HCC). Recent studies have evaluated the early diagnosis of primary HCC through analysis of gut microbiota dysbiosis. However, the relationship between the degree of dysbiosis and the prognosis of primary HCC remains unclear. Because primary HCC is accompanied by dysbiosis and dysbiosis usually increases the circulatory concentrations of endotoxin and other harmful bacterial substances, which further increases liver damage, we hypothesized that level of dysbiosis associated with primary HCC increases with the stage of cancer progression. To test this hypothesis, we introduced a more integrated index referred to as the degree of dysbiosis (Ddys); and we investigated Ddys of the gut microbiota with the development of primary HCC through high-throughput sequencing of 16S rRNA gene amplicons. Our results showed that compared with healthy individuals, patients with primary HCC showed increased pro-inflammatory bacteria in their fecal microbiota. The Ddys increased significantly in patients with primary HCC compared with that in healthy controls. Moreover, there was a tendency for the Ddys to increase with the development of primary HCC, although no significant difference was detected between different stages of primary HCC. Our findings provide important insights into the use of gut microbiota analysis during the treatment of primary HCC.https://www.frontiersin.org/article/10.3389/fmicb.2019.01458/fullchronic liver diseasesdysbiosis degreegut microbiotaprimary hepatocellular carcinomaprognosis |
| spellingShingle | Jiajia Ni Jiajia Ni Rong Huang Huifang Zhou Xiaoping Xu Xiaoping Xu Yang Li Yang Li Peihua Cao Peihua Cao Kebo Zhong Kebo Zhong Mei Ge Mei Ge Xiaoxia Chen Xiaoxia Chen Baohua Hou Min Yu Baogang Peng Qiao Li Peng Zhang Yi Gao Yi Gao Analysis of the Relationship Between the Degree of Dysbiosis in Gut Microbiota and Prognosis at Different Stages of Primary Hepatocellular Carcinoma Frontiers in Microbiology chronic liver diseases dysbiosis degree gut microbiota primary hepatocellular carcinoma prognosis |
| title | Analysis of the Relationship Between the Degree of Dysbiosis in Gut Microbiota and Prognosis at Different Stages of Primary Hepatocellular Carcinoma |
| title_full | Analysis of the Relationship Between the Degree of Dysbiosis in Gut Microbiota and Prognosis at Different Stages of Primary Hepatocellular Carcinoma |
| title_fullStr | Analysis of the Relationship Between the Degree of Dysbiosis in Gut Microbiota and Prognosis at Different Stages of Primary Hepatocellular Carcinoma |
| title_full_unstemmed | Analysis of the Relationship Between the Degree of Dysbiosis in Gut Microbiota and Prognosis at Different Stages of Primary Hepatocellular Carcinoma |
| title_short | Analysis of the Relationship Between the Degree of Dysbiosis in Gut Microbiota and Prognosis at Different Stages of Primary Hepatocellular Carcinoma |
| title_sort | analysis of the relationship between the degree of dysbiosis in gut microbiota and prognosis at different stages of primary hepatocellular carcinoma |
| topic | chronic liver diseases dysbiosis degree gut microbiota primary hepatocellular carcinoma prognosis |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01458/full |
| work_keys_str_mv | AT jiajiani analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT jiajiani analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT ronghuang analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT huifangzhou analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT xiaopingxu analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT xiaopingxu analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT yangli analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT yangli analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT peihuacao analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT peihuacao analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT kebozhong analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT kebozhong analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT meige analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT meige analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT xiaoxiachen analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT xiaoxiachen analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT baohuahou analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT minyu analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT baogangpeng analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT qiaoli analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT pengzhang analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT yigao analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma AT yigao analysisoftherelationshipbetweenthedegreeofdysbiosisingutmicrobiotaandprognosisatdifferentstagesofprimaryhepatocellularcarcinoma |