Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats
Objective: To explore the effects of kelulut honey on bone structure and histomorphometry against glucocorticoid-induced osteoporosis. Methods: Thirty-five male rats were used (n = 7). Twenty-eight adrenalectomized rats were divided into four groups; each group was given normal saline 0.9% (negative...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=12;spage=493;epage=500;aulast=Kamaruzzaman |
| _version_ | 1819135732008091648 |
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| author | Mohd Amir Kamaruzzaman Amardev Thanu Mohd Rafizul Yusof Ima Nirwana Soelaiman Elvy Suhana Ramli |
| author_facet | Mohd Amir Kamaruzzaman Amardev Thanu Mohd Rafizul Yusof Ima Nirwana Soelaiman Elvy Suhana Ramli |
| author_sort | Mohd Amir Kamaruzzaman |
| collection | DOAJ |
| description | Objective: To explore the effects of kelulut honey on bone structure and histomorphometry against glucocorticoid-induced osteoporosis.
Methods: Thirty-five male rats were used (n = 7). Twenty-eight adrenalectomized rats were divided into four groups; each group was given normal saline 0.9% (negative control), calcium water (positive control), kelulut honey (200 mg/kg/day and 400 mg/kg/day, respectively) treatment, respectively. All of them were administered with intramuscular injection of dexamethasone (120 μg/kg/day) to induce osteoporosis. Seven sham operated rats were given vehicle palm olein 0.05 mL/100 g/day by intramuscular injection and 0.1 mL/kg/day orally. All the treatments were given daily for 2 month. Lipid peroxidation and oxidative stress enzymes were measured. In addition, bone structural and histomorphometry analyses were also conducted.
Results: Two-month glucocorticoid treatment increased the level of malondialdehyde and decreased superoxide dismutase significantly. No significant changes were found in the activities of catalase and glutathion peroxidase. Bone volume/tissue volume and trabecular number were significantly reduced while trabecular separation of the femoral bones was increased which corresponded to the decreased number of osteoblast surface after two months of receiving glucocorticoid treatment. Kelulut honey treatment restored the level of superoxide dismutase and reduced malondialdehyde significantly (P<0.05). Moreover, kelulut honey increased bone volume/tissue volume, trabecular number and decreased trabecular separation significantly (P<0.05), which were further confirmed by increased osteoblast surface and decreased osteoclast surface number (P<0.05).
Conclusions: Kelulut honey may have potential bone protective effect, and may be a prophylaxis against glucocorticoid-induced osteoporosis. |
| first_indexed | 2024-12-22T10:23:45Z |
| format | Article |
| id | doaj.art-a4fc03399d264de993ae1f29eb72c9b1 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 2588-9222 |
| language | English |
| last_indexed | 2024-12-22T10:23:45Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-a4fc03399d264de993ae1f29eb72c9b12022-12-21T18:29:33ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222019-01-0191249350010.4103/2221-1691.271722Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in ratsMohd Amir KamaruzzamanAmardev ThanuMohd Rafizul YusofIma Nirwana SoelaimanElvy Suhana RamliObjective: To explore the effects of kelulut honey on bone structure and histomorphometry against glucocorticoid-induced osteoporosis. Methods: Thirty-five male rats were used (n = 7). Twenty-eight adrenalectomized rats were divided into four groups; each group was given normal saline 0.9% (negative control), calcium water (positive control), kelulut honey (200 mg/kg/day and 400 mg/kg/day, respectively) treatment, respectively. All of them were administered with intramuscular injection of dexamethasone (120 μg/kg/day) to induce osteoporosis. Seven sham operated rats were given vehicle palm olein 0.05 mL/100 g/day by intramuscular injection and 0.1 mL/kg/day orally. All the treatments were given daily for 2 month. Lipid peroxidation and oxidative stress enzymes were measured. In addition, bone structural and histomorphometry analyses were also conducted. Results: Two-month glucocorticoid treatment increased the level of malondialdehyde and decreased superoxide dismutase significantly. No significant changes were found in the activities of catalase and glutathion peroxidase. Bone volume/tissue volume and trabecular number were significantly reduced while trabecular separation of the femoral bones was increased which corresponded to the decreased number of osteoblast surface after two months of receiving glucocorticoid treatment. Kelulut honey treatment restored the level of superoxide dismutase and reduced malondialdehyde significantly (P<0.05). Moreover, kelulut honey increased bone volume/tissue volume, trabecular number and decreased trabecular separation significantly (P<0.05), which were further confirmed by increased osteoblast surface and decreased osteoclast surface number (P<0.05). Conclusions: Kelulut honey may have potential bone protective effect, and may be a prophylaxis against glucocorticoid-induced osteoporosis.http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=12;spage=493;epage=500;aulast=Kamaruzzamankelulut honeyglucocorticoid-induced osteoporosisoxidative stressantioxidantbone structurehistomorphometrymale rats |
| spellingShingle | Mohd Amir Kamaruzzaman Amardev Thanu Mohd Rafizul Yusof Ima Nirwana Soelaiman Elvy Suhana Ramli Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats Asian Pacific Journal of Tropical Biomedicine kelulut honey glucocorticoid-induced osteoporosis oxidative stress antioxidant bone structure histomorphometry male rats |
| title | Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats |
| title_full | Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats |
| title_fullStr | Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats |
| title_full_unstemmed | Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats |
| title_short | Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats |
| title_sort | kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats |
| topic | kelulut honey glucocorticoid-induced osteoporosis oxidative stress antioxidant bone structure histomorphometry male rats |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=12;spage=493;epage=500;aulast=Kamaruzzaman |
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