Gonadal Transcriptome Analysis in Sterile Double Haploid Japanese Flounder.
Sterility is a serious problem that can affect all bionts. In teleosts, double haploids (DHs) induced by mitogynogenesis are often sterile. This sterility severely restricts the further application of DHs for production of clones, genetic analysis, and breeding. However, sterile DH individuals are g...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4651314?pdf=render |
| _version_ | 1818132505683820544 |
|---|---|
| author | Xiaoyan Zhang Jilun Hou Guixing Wang Hongbo Jiang Yufen Wang Zhaohui Sun Xiufeng Jiang Qinghai Yu Haijin Liu |
| author_facet | Xiaoyan Zhang Jilun Hou Guixing Wang Hongbo Jiang Yufen Wang Zhaohui Sun Xiufeng Jiang Qinghai Yu Haijin Liu |
| author_sort | Xiaoyan Zhang |
| collection | DOAJ |
| description | Sterility is a serious problem that can affect all bionts. In teleosts, double haploids (DHs) induced by mitogynogenesis are often sterile. This sterility severely restricts the further application of DHs for production of clones, genetic analysis, and breeding. However, sterile DH individuals are good source materials for investigation of the molecular mechanisms of gonad development, especially for studies into the role of genes that are indispensable for fish reproduction. Here, we used the Illumina sequencing platform to analyze the transcriptome of sterile female DH Japanese flounder in order to identify major genes that cause sterility and to provide a molecular basis for an intensive study of gonadal development in teleosts. Through sequencing, assembly, and annotation, we obtained 52,474 contigs and found that 60.7% of these shared homologies with existing sequences. A total of 1225 differentially expressed unigenes were found, including 492 upregulated and 733 downregulated genes. Gene Ontology and KEGG analyses showed that genes showing significant upregulation, such as CYP11A1, CYP11B2, CYP17, CYP21, HSD3β, bcl2l1, and PRLR, principally correlated with sterol metabolic process, steroid biosynthetic process, and the Jak-stat signaling pathway. The significantly downregulated genes were primarily associated with immune response, antigen processing and presentation, cytokine-cytokine receptor interaction, and protein digestion and absorption. Using a co-expression network analysis, we conducted a comprehensive comparison of gene expression in the gonads of fertile and sterile female DH Japanese flounder. Identification of genes showing significantly different expression will provide further insights into DH reproductive dysfunction and oocyte maturation processes in teleosts. |
| first_indexed | 2024-12-11T08:37:54Z |
| format | Article |
| id | doaj.art-a4fe3fc2b0eb40bc931dd5c7107671bf |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T08:37:54Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a4fe3fc2b0eb40bc931dd5c7107671bf2022-12-22T01:14:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011011e014320410.1371/journal.pone.0143204Gonadal Transcriptome Analysis in Sterile Double Haploid Japanese Flounder.Xiaoyan ZhangJilun HouGuixing WangHongbo JiangYufen WangZhaohui SunXiufeng JiangQinghai YuHaijin LiuSterility is a serious problem that can affect all bionts. In teleosts, double haploids (DHs) induced by mitogynogenesis are often sterile. This sterility severely restricts the further application of DHs for production of clones, genetic analysis, and breeding. However, sterile DH individuals are good source materials for investigation of the molecular mechanisms of gonad development, especially for studies into the role of genes that are indispensable for fish reproduction. Here, we used the Illumina sequencing platform to analyze the transcriptome of sterile female DH Japanese flounder in order to identify major genes that cause sterility and to provide a molecular basis for an intensive study of gonadal development in teleosts. Through sequencing, assembly, and annotation, we obtained 52,474 contigs and found that 60.7% of these shared homologies with existing sequences. A total of 1225 differentially expressed unigenes were found, including 492 upregulated and 733 downregulated genes. Gene Ontology and KEGG analyses showed that genes showing significant upregulation, such as CYP11A1, CYP11B2, CYP17, CYP21, HSD3β, bcl2l1, and PRLR, principally correlated with sterol metabolic process, steroid biosynthetic process, and the Jak-stat signaling pathway. The significantly downregulated genes were primarily associated with immune response, antigen processing and presentation, cytokine-cytokine receptor interaction, and protein digestion and absorption. Using a co-expression network analysis, we conducted a comprehensive comparison of gene expression in the gonads of fertile and sterile female DH Japanese flounder. Identification of genes showing significantly different expression will provide further insights into DH reproductive dysfunction and oocyte maturation processes in teleosts.http://europepmc.org/articles/PMC4651314?pdf=render |
| spellingShingle | Xiaoyan Zhang Jilun Hou Guixing Wang Hongbo Jiang Yufen Wang Zhaohui Sun Xiufeng Jiang Qinghai Yu Haijin Liu Gonadal Transcriptome Analysis in Sterile Double Haploid Japanese Flounder. PLoS ONE |
| title | Gonadal Transcriptome Analysis in Sterile Double Haploid Japanese Flounder. |
| title_full | Gonadal Transcriptome Analysis in Sterile Double Haploid Japanese Flounder. |
| title_fullStr | Gonadal Transcriptome Analysis in Sterile Double Haploid Japanese Flounder. |
| title_full_unstemmed | Gonadal Transcriptome Analysis in Sterile Double Haploid Japanese Flounder. |
| title_short | Gonadal Transcriptome Analysis in Sterile Double Haploid Japanese Flounder. |
| title_sort | gonadal transcriptome analysis in sterile double haploid japanese flounder |
| url | http://europepmc.org/articles/PMC4651314?pdf=render |
| work_keys_str_mv | AT xiaoyanzhang gonadaltranscriptomeanalysisinsteriledoublehaploidjapaneseflounder AT jilunhou gonadaltranscriptomeanalysisinsteriledoublehaploidjapaneseflounder AT guixingwang gonadaltranscriptomeanalysisinsteriledoublehaploidjapaneseflounder AT hongbojiang gonadaltranscriptomeanalysisinsteriledoublehaploidjapaneseflounder AT yufenwang gonadaltranscriptomeanalysisinsteriledoublehaploidjapaneseflounder AT zhaohuisun gonadaltranscriptomeanalysisinsteriledoublehaploidjapaneseflounder AT xiufengjiang gonadaltranscriptomeanalysisinsteriledoublehaploidjapaneseflounder AT qinghaiyu gonadaltranscriptomeanalysisinsteriledoublehaploidjapaneseflounder AT haijinliu gonadaltranscriptomeanalysisinsteriledoublehaploidjapaneseflounder |