Cryostructuring of Polymeric Systems: 64. Preparation and Properties of Poly(vinyl alcohol)-Based Cryogels Loaded with Antimicrobial Drugs and Assessment of the Potential of Such Gel Materials to Perform as Gel Implants for the Treatment of Infected Wounds
Physical macroporous poly(vinyl alcohol)-based cryogels formed by the freeze–thaw technique without the use of any foreign cross-linkers are of significant interests for biomedical applications. In the present study, such gel materials loaded with the antimicrobial substances were prepared and their...
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MDPI AG
2023-01-01
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| author | Olga Yu. Kolosova Astemir I. Shaikhaliev Mikhail S. Krasnov Ivan M. Bondar Egor V. Sidorskii Elena V. Sorokina Vladimir I. Lozinsky |
| author_facet | Olga Yu. Kolosova Astemir I. Shaikhaliev Mikhail S. Krasnov Ivan M. Bondar Egor V. Sidorskii Elena V. Sorokina Vladimir I. Lozinsky |
| author_sort | Olga Yu. Kolosova |
| collection | DOAJ |
| description | Physical macroporous poly(vinyl alcohol)-based cryogels formed by the freeze–thaw technique without the use of any foreign cross-linkers are of significant interests for biomedical applications. In the present study, such gel materials loaded with the antimicrobial substances were prepared and their physicochemical properties were evaluated followed by an assessment of their potential to serve as drug carriers that can be used as implants for the treatment of infected wounds. The antibiotic Ceftriaxone and the antimycotic Fluconazole were used as antimicrobial agents. It was shown that the Ceftriaxone additives caused the up-swelling effects with respect to the cryogel matrix and some decrease in its heat endurance but did not result in a substantial change in the gel strength. With that, the drug release from the cryogel vehicle occurred without any diffusion restrictions, which was demonstrated by both the spectrophotometric recording and the microbiological agar diffusion technique. In turn, the in vivo biotesting of such drug-loaded cryogels also showed that these materials were able to function as rather efficient antimicrobial implants injected in the artificially infected model wounds of laboratory rabbits. These results confirmed the promising biomedical potential of similar implants. |
| first_indexed | 2024-03-11T08:48:08Z |
| format | Article |
| id | doaj.art-a503d9325a74494a8c2e5aa308d54ef9 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-11T08:48:08Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-a503d9325a74494a8c2e5aa308d54ef92023-11-16T20:39:23ZengMDPI AGGels2310-28612023-01-019211310.3390/gels9020113Cryostructuring of Polymeric Systems: 64. Preparation and Properties of Poly(vinyl alcohol)-Based Cryogels Loaded with Antimicrobial Drugs and Assessment of the Potential of Such Gel Materials to Perform as Gel Implants for the Treatment of Infected WoundsOlga Yu. Kolosova0Astemir I. Shaikhaliev1Mikhail S. Krasnov2Ivan M. Bondar3Egor V. Sidorskii4Elena V. Sorokina5Vladimir I. Lozinsky6A.N.Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Vavilov Street 28, Bld. 1, 119334 Moscow, RussiaInstitute of Dentistry, I.M.Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaA.N.Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Vavilov Street 28, Bld. 1, 119334 Moscow, RussiaInstitute of Dentistry, I.M.Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaA.N.Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Vavilov Street 28, Bld. 1, 119334 Moscow, RussiaMicrobiology Department, Biology Faculty, M.V.Lomonosov Moscow State University, 119991 Moscow, RussiaA.N.Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Vavilov Street 28, Bld. 1, 119334 Moscow, RussiaPhysical macroporous poly(vinyl alcohol)-based cryogels formed by the freeze–thaw technique without the use of any foreign cross-linkers are of significant interests for biomedical applications. In the present study, such gel materials loaded with the antimicrobial substances were prepared and their physicochemical properties were evaluated followed by an assessment of their potential to serve as drug carriers that can be used as implants for the treatment of infected wounds. The antibiotic Ceftriaxone and the antimycotic Fluconazole were used as antimicrobial agents. It was shown that the Ceftriaxone additives caused the up-swelling effects with respect to the cryogel matrix and some decrease in its heat endurance but did not result in a substantial change in the gel strength. With that, the drug release from the cryogel vehicle occurred without any diffusion restrictions, which was demonstrated by both the spectrophotometric recording and the microbiological agar diffusion technique. In turn, the in vivo biotesting of such drug-loaded cryogels also showed that these materials were able to function as rather efficient antimicrobial implants injected in the artificially infected model wounds of laboratory rabbits. These results confirmed the promising biomedical potential of similar implants.https://www.mdpi.com/2310-2861/9/2/113poly(vinyl alcohol) cryogelCeftriaxoneFluconazoledrug releaseantimicrobial implantsin vitro and in vivo bioassay |
| spellingShingle | Olga Yu. Kolosova Astemir I. Shaikhaliev Mikhail S. Krasnov Ivan M. Bondar Egor V. Sidorskii Elena V. Sorokina Vladimir I. Lozinsky Cryostructuring of Polymeric Systems: 64. Preparation and Properties of Poly(vinyl alcohol)-Based Cryogels Loaded with Antimicrobial Drugs and Assessment of the Potential of Such Gel Materials to Perform as Gel Implants for the Treatment of Infected Wounds Gels poly(vinyl alcohol) cryogel Ceftriaxone Fluconazole drug release antimicrobial implants in vitro and in vivo bioassay |
| title | Cryostructuring of Polymeric Systems: 64. Preparation and Properties of Poly(vinyl alcohol)-Based Cryogels Loaded with Antimicrobial Drugs and Assessment of the Potential of Such Gel Materials to Perform as Gel Implants for the Treatment of Infected Wounds |
| title_full | Cryostructuring of Polymeric Systems: 64. Preparation and Properties of Poly(vinyl alcohol)-Based Cryogels Loaded with Antimicrobial Drugs and Assessment of the Potential of Such Gel Materials to Perform as Gel Implants for the Treatment of Infected Wounds |
| title_fullStr | Cryostructuring of Polymeric Systems: 64. Preparation and Properties of Poly(vinyl alcohol)-Based Cryogels Loaded with Antimicrobial Drugs and Assessment of the Potential of Such Gel Materials to Perform as Gel Implants for the Treatment of Infected Wounds |
| title_full_unstemmed | Cryostructuring of Polymeric Systems: 64. Preparation and Properties of Poly(vinyl alcohol)-Based Cryogels Loaded with Antimicrobial Drugs and Assessment of the Potential of Such Gel Materials to Perform as Gel Implants for the Treatment of Infected Wounds |
| title_short | Cryostructuring of Polymeric Systems: 64. Preparation and Properties of Poly(vinyl alcohol)-Based Cryogels Loaded with Antimicrobial Drugs and Assessment of the Potential of Such Gel Materials to Perform as Gel Implants for the Treatment of Infected Wounds |
| title_sort | cryostructuring of polymeric systems 64 preparation and properties of poly vinyl alcohol based cryogels loaded with antimicrobial drugs and assessment of the potential of such gel materials to perform as gel implants for the treatment of infected wounds |
| topic | poly(vinyl alcohol) cryogel Ceftriaxone Fluconazole drug release antimicrobial implants in vitro and in vivo bioassay |
| url | https://www.mdpi.com/2310-2861/9/2/113 |
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