Characterization of Endothelial Cells Associated with Hematopoietic Niche Formation in Humans Identifies IL-33 As an Anabolic Factor
Bone marrow formation requires an orchestrated interplay between osteogenesis, angiogenesis, and hematopoiesis that is thought to be mediated by endothelial cells. The nature of the endothelial cells and the molecular mechanisms underlying these events remain unclear in humans. Here, we identify a s...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717319071 |
| _version_ | 1818692458409623552 |
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| author | Keane Jared Guillaume Kenswil Adrian Christopher Jaramillo Zhen Ping Si Chen Remco Michiel Hoogenboezem Maria Athina Mylona Maria Niken Adisty Eric Moniqué Johannes Bindels Pieter Koen Bos Hans Stoop King Hong Lam Bram van Eerden Tom Cupedo Marc Hermanus Gerardus Petrus Raaijmakers |
| author_facet | Keane Jared Guillaume Kenswil Adrian Christopher Jaramillo Zhen Ping Si Chen Remco Michiel Hoogenboezem Maria Athina Mylona Maria Niken Adisty Eric Moniqué Johannes Bindels Pieter Koen Bos Hans Stoop King Hong Lam Bram van Eerden Tom Cupedo Marc Hermanus Gerardus Petrus Raaijmakers |
| author_sort | Keane Jared Guillaume Kenswil |
| collection | DOAJ |
| description | Bone marrow formation requires an orchestrated interplay between osteogenesis, angiogenesis, and hematopoiesis that is thought to be mediated by endothelial cells. The nature of the endothelial cells and the molecular mechanisms underlying these events remain unclear in humans. Here, we identify a subset of endoglin-expressing endothelial cells enriched in human bone marrow during fetal ontogeny and upon regeneration after chemotherapeutic injury. Comprehensive transcriptional characterization by massive parallel RNA sequencing of these cells reveals a phenotypic and molecular similarity to murine type H endothelium and activation of angiocrine factors implicated in hematopoiesis, osteogenesis, and angiogenesis. Interleukin-33 (IL-33) was significantly overexpressed in these endothelial cells and promoted the expansion of distinct subsets of hematopoietic precursor cells, endothelial cells, as well as osteogenic differentiation. The identification and molecular characterization of these human regeneration-associated endothelial cells is thus anticipated to instruct the discovery of angiocrine factors driving bone marrow formation and recovery after injury. |
| first_indexed | 2024-12-17T12:58:07Z |
| format | Article |
| id | doaj.art-a5063066a1904e7097ddea94317be738 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-17T12:58:07Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-a5063066a1904e7097ddea94317be7382022-12-21T21:47:26ZengElsevierCell Reports2211-12472018-01-0122366667810.1016/j.celrep.2017.12.070Characterization of Endothelial Cells Associated with Hematopoietic Niche Formation in Humans Identifies IL-33 As an Anabolic FactorKeane Jared Guillaume Kenswil0Adrian Christopher Jaramillo1Zhen Ping2Si Chen3Remco Michiel Hoogenboezem4Maria Athina Mylona5Maria Niken Adisty6Eric Moniqué Johannes Bindels7Pieter Koen Bos8Hans Stoop9King Hong Lam10Bram van Eerden11Tom Cupedo12Marc Hermanus Gerardus Petrus Raaijmakers13Department of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015CN, the NetherlandsDepartment of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015CN, the NetherlandsDepartment of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015CN, the NetherlandsDepartment of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015CN, the NetherlandsDepartment of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015CN, the NetherlandsDepartment of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015CN, the NetherlandsDepartment of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015CN, the NetherlandsDepartment of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015CN, the NetherlandsDepartment of Orthopaedics, Erasmus MC, 3015 CE Rotterdam, the NetherlandsDepartment of Pathology, Erasmus MC Cancer Institute, Rotterdam 3015CN, the NetherlandsDepartment of Pathology, Erasmus MC Cancer Institute, Rotterdam 3015CN, the NetherlandsDepartment of Internal Medicine, Erasmus MC Cancer Institute, Rotterdam 3015CN, the NetherlandsDepartment of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015CN, the NetherlandsDepartment of Hematology, Erasmus MC Cancer Institute, Rotterdam 3015CN, the NetherlandsBone marrow formation requires an orchestrated interplay between osteogenesis, angiogenesis, and hematopoiesis that is thought to be mediated by endothelial cells. The nature of the endothelial cells and the molecular mechanisms underlying these events remain unclear in humans. Here, we identify a subset of endoglin-expressing endothelial cells enriched in human bone marrow during fetal ontogeny and upon regeneration after chemotherapeutic injury. Comprehensive transcriptional characterization by massive parallel RNA sequencing of these cells reveals a phenotypic and molecular similarity to murine type H endothelium and activation of angiocrine factors implicated in hematopoiesis, osteogenesis, and angiogenesis. Interleukin-33 (IL-33) was significantly overexpressed in these endothelial cells and promoted the expansion of distinct subsets of hematopoietic precursor cells, endothelial cells, as well as osteogenic differentiation. The identification and molecular characterization of these human regeneration-associated endothelial cells is thus anticipated to instruct the discovery of angiocrine factors driving bone marrow formation and recovery after injury.http://www.sciencedirect.com/science/article/pii/S2211124717319071endothelialbone marrownicheregenerationdevelopmenthematopoietic stem/progenitor cellbone formationinterleukin-33 |
| spellingShingle | Keane Jared Guillaume Kenswil Adrian Christopher Jaramillo Zhen Ping Si Chen Remco Michiel Hoogenboezem Maria Athina Mylona Maria Niken Adisty Eric Moniqué Johannes Bindels Pieter Koen Bos Hans Stoop King Hong Lam Bram van Eerden Tom Cupedo Marc Hermanus Gerardus Petrus Raaijmakers Characterization of Endothelial Cells Associated with Hematopoietic Niche Formation in Humans Identifies IL-33 As an Anabolic Factor Cell Reports endothelial bone marrow niche regeneration development hematopoietic stem/progenitor cell bone formation interleukin-33 |
| title | Characterization of Endothelial Cells Associated with Hematopoietic Niche Formation in Humans Identifies IL-33 As an Anabolic Factor |
| title_full | Characterization of Endothelial Cells Associated with Hematopoietic Niche Formation in Humans Identifies IL-33 As an Anabolic Factor |
| title_fullStr | Characterization of Endothelial Cells Associated with Hematopoietic Niche Formation in Humans Identifies IL-33 As an Anabolic Factor |
| title_full_unstemmed | Characterization of Endothelial Cells Associated with Hematopoietic Niche Formation in Humans Identifies IL-33 As an Anabolic Factor |
| title_short | Characterization of Endothelial Cells Associated with Hematopoietic Niche Formation in Humans Identifies IL-33 As an Anabolic Factor |
| title_sort | characterization of endothelial cells associated with hematopoietic niche formation in humans identifies il 33 as an anabolic factor |
| topic | endothelial bone marrow niche regeneration development hematopoietic stem/progenitor cell bone formation interleukin-33 |
| url | http://www.sciencedirect.com/science/article/pii/S2211124717319071 |
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