Detection of Viable and Total Bacterial Community in the Pit Mud of Chinese Strong-Flavor Liquor Using Propidium Monoazide Combined With Quantitative PCR and 16S rRNA Gene Sequencing

Detection of Viable and Total Bacterial Community in the Pit Mud of Chinese Strong-Flavor Liquor Using Propidium Monoazide Combined With Quantitative PCR and 16S rRNA Gene Sequencing

Microbiota in the pit mud (PM) plays a crucial role in the production of Chinese strong-flavor liquor (CSFL), the most popular distilled liquor in China. However, previous studies used total microbes, instead of viable ones, for the characterization of the microbial community in this environment. In...

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Bibliographic Details
Main Authors: Guangxun Tan, Rui Zhou, Wenqian Zhang, Yuanliang Hu, Zhiyong Ruan, Jing Li, Changyi Zhang, Dengjin Shen, Nan Peng, Yunxiang Liang, Shumiao Zhao
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-05-01
Series:Frontiers in Microbiology
Subjects:
16S rRNA gene sequencing
Chinese strong-flavor liquor
pit mud
propidium monoazide (PMA)
viable microbe detection
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2020.00896/full

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