Clindamycin-Loaded Halloysite Nanotubes as the Antibacterial Component of Composite Hydrogel for Bone Repair
A new drug delivery system consisting of clindamycin phosphate entrapped in acid-etched halloysite nanotubes was successfully prepared and characterized. It was then used as an antibacterial component of the multicomponent hydrogel designed as a material for bone regeneration. First, halloysite (HNT...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/23/5151 |
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| author | Adrianna Machowska Joanna Klara Gabriela Ledwójcik Kinga Wójcik Joanna Dulińska-Litewka Anna Karewicz |
| author_facet | Adrianna Machowska Joanna Klara Gabriela Ledwójcik Kinga Wójcik Joanna Dulińska-Litewka Anna Karewicz |
| author_sort | Adrianna Machowska |
| collection | DOAJ |
| description | A new drug delivery system consisting of clindamycin phosphate entrapped in acid-etched halloysite nanotubes was successfully prepared and characterized. It was then used as an antibacterial component of the multicomponent hydrogel designed as a material for bone regeneration. First, halloysite (HNT) was etched and clindamycin phosphate (CP) was entrapped in both raw and modified nanotubes, resulting in HNT-CP and EHNT-CP systems. They were characterized using SEM, TEM, TGA and FTIR; the entrapment efficiency and release of CP from both systems were also studied. EHNT-CP was then used as an antibacterial component of the two hydrogels composed of alginate, collagen and β-TCP. The hydrogels were prepared using different crosslinking procedures but had the same composition. The morphology, porosity, degradation rate, CP release profile, cytocompatibility, antibacterial activity and ability to induce biomineralization were studied for both materials. The hydrogel obtained by a chemical crosslinking with EDC followed by the physical crosslinking with calcium ions had better properties and was shown to have potential as a bone repair material. |
| first_indexed | 2024-03-09T17:35:01Z |
| format | Article |
| id | doaj.art-a50957d2c9444426b395bbf9c53b075f |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T17:35:01Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-a50957d2c9444426b395bbf9c53b075f2023-11-24T11:59:14ZengMDPI AGPolymers2073-43602022-11-011423515110.3390/polym14235151Clindamycin-Loaded Halloysite Nanotubes as the Antibacterial Component of Composite Hydrogel for Bone RepairAdrianna Machowska0Joanna Klara1Gabriela Ledwójcik2Kinga Wójcik3Joanna Dulińska-Litewka4Anna Karewicz5Department of Chemistry, Jagiellonian University, 2 Gronostajowa St., 30-387 Kraków, PolandDepartment of Chemistry, Jagiellonian University, 2 Gronostajowa St., 30-387 Kraków, PolandDepartment of Chemistry, Jagiellonian University, 2 Gronostajowa St., 30-387 Kraków, PolandDepartment of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, PolandChair of Medical Biochemistry, Faculty of Medicine, Medical College, Jagiellonian University, 31-034 Kraków, PolandDepartment of Chemistry, Jagiellonian University, 2 Gronostajowa St., 30-387 Kraków, PolandA new drug delivery system consisting of clindamycin phosphate entrapped in acid-etched halloysite nanotubes was successfully prepared and characterized. It was then used as an antibacterial component of the multicomponent hydrogel designed as a material for bone regeneration. First, halloysite (HNT) was etched and clindamycin phosphate (CP) was entrapped in both raw and modified nanotubes, resulting in HNT-CP and EHNT-CP systems. They were characterized using SEM, TEM, TGA and FTIR; the entrapment efficiency and release of CP from both systems were also studied. EHNT-CP was then used as an antibacterial component of the two hydrogels composed of alginate, collagen and β-TCP. The hydrogels were prepared using different crosslinking procedures but had the same composition. The morphology, porosity, degradation rate, CP release profile, cytocompatibility, antibacterial activity and ability to induce biomineralization were studied for both materials. The hydrogel obtained by a chemical crosslinking with EDC followed by the physical crosslinking with calcium ions had better properties and was shown to have potential as a bone repair material.https://www.mdpi.com/2073-4360/14/23/5151halloysiteclindamycinhydrogel crosslinkingalginatecollagenβ-TCP |
| spellingShingle | Adrianna Machowska Joanna Klara Gabriela Ledwójcik Kinga Wójcik Joanna Dulińska-Litewka Anna Karewicz Clindamycin-Loaded Halloysite Nanotubes as the Antibacterial Component of Composite Hydrogel for Bone Repair Polymers halloysite clindamycin hydrogel crosslinking alginate collagen β-TCP |
| title | Clindamycin-Loaded Halloysite Nanotubes as the Antibacterial Component of Composite Hydrogel for Bone Repair |
| title_full | Clindamycin-Loaded Halloysite Nanotubes as the Antibacterial Component of Composite Hydrogel for Bone Repair |
| title_fullStr | Clindamycin-Loaded Halloysite Nanotubes as the Antibacterial Component of Composite Hydrogel for Bone Repair |
| title_full_unstemmed | Clindamycin-Loaded Halloysite Nanotubes as the Antibacterial Component of Composite Hydrogel for Bone Repair |
| title_short | Clindamycin-Loaded Halloysite Nanotubes as the Antibacterial Component of Composite Hydrogel for Bone Repair |
| title_sort | clindamycin loaded halloysite nanotubes as the antibacterial component of composite hydrogel for bone repair |
| topic | halloysite clindamycin hydrogel crosslinking alginate collagen β-TCP |
| url | https://www.mdpi.com/2073-4360/14/23/5151 |
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