Rapid antibiotic susceptibility testing from blood culture bottles with species agnostic real-time polymerase chain reaction.
Development and implementation of rapid antimicrobial susceptibility testing is critical for guiding patient care and improving clinical outcomes, especially in cases of sepsis. One approach to reduce the time-to-answer for antimicrobial susceptibility is monitoring the inhibition of DNA production,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0209042 |
| _version_ | 1831760824799068160 |
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| author | Tucker Maxson Candace D Blancett Amanda S Graham Christopher P Stefan Timothy D Minogue |
| author_facet | Tucker Maxson Candace D Blancett Amanda S Graham Christopher P Stefan Timothy D Minogue |
| author_sort | Tucker Maxson |
| collection | DOAJ |
| description | Development and implementation of rapid antimicrobial susceptibility testing is critical for guiding patient care and improving clinical outcomes, especially in cases of sepsis. One approach to reduce the time-to-answer for antimicrobial susceptibility is monitoring the inhibition of DNA production, as differences in DNA concentrations are more quickly impacted compared to optical density changes in traditional antimicrobial susceptibility testing. Here, we use real-time PCR to rapidly determine antimicrobial susceptibility after short incubations with antibiotic. Application of this assay to a collection of 144 isolates in mock blood culture, covering medically relevant pathogens displaying high rates of resistance, provided susceptibility data in under 4 hours. This assay provided categorical agreement with a reference method in 96.3% of cases across all species. Sequencing of a subset of PCR amplicons showed accurate genus level identification. Overall, implementation of this method could provide accurate susceptibility results with a reduced time-to-answer for a number of medically relevant bacteria commonly isolated from blood culture. |
| first_indexed | 2024-12-22T04:37:11Z |
| format | Article |
| id | doaj.art-a518383e883643abacda2735babe27e1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T04:37:11Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a518383e883643abacda2735babe27e12022-12-21T18:38:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011312e020904210.1371/journal.pone.0209042Rapid antibiotic susceptibility testing from blood culture bottles with species agnostic real-time polymerase chain reaction.Tucker MaxsonCandace D BlancettAmanda S GrahamChristopher P StefanTimothy D MinogueDevelopment and implementation of rapid antimicrobial susceptibility testing is critical for guiding patient care and improving clinical outcomes, especially in cases of sepsis. One approach to reduce the time-to-answer for antimicrobial susceptibility is monitoring the inhibition of DNA production, as differences in DNA concentrations are more quickly impacted compared to optical density changes in traditional antimicrobial susceptibility testing. Here, we use real-time PCR to rapidly determine antimicrobial susceptibility after short incubations with antibiotic. Application of this assay to a collection of 144 isolates in mock blood culture, covering medically relevant pathogens displaying high rates of resistance, provided susceptibility data in under 4 hours. This assay provided categorical agreement with a reference method in 96.3% of cases across all species. Sequencing of a subset of PCR amplicons showed accurate genus level identification. Overall, implementation of this method could provide accurate susceptibility results with a reduced time-to-answer for a number of medically relevant bacteria commonly isolated from blood culture.https://doi.org/10.1371/journal.pone.0209042 |
| spellingShingle | Tucker Maxson Candace D Blancett Amanda S Graham Christopher P Stefan Timothy D Minogue Rapid antibiotic susceptibility testing from blood culture bottles with species agnostic real-time polymerase chain reaction. PLoS ONE |
| title | Rapid antibiotic susceptibility testing from blood culture bottles with species agnostic real-time polymerase chain reaction. |
| title_full | Rapid antibiotic susceptibility testing from blood culture bottles with species agnostic real-time polymerase chain reaction. |
| title_fullStr | Rapid antibiotic susceptibility testing from blood culture bottles with species agnostic real-time polymerase chain reaction. |
| title_full_unstemmed | Rapid antibiotic susceptibility testing from blood culture bottles with species agnostic real-time polymerase chain reaction. |
| title_short | Rapid antibiotic susceptibility testing from blood culture bottles with species agnostic real-time polymerase chain reaction. |
| title_sort | rapid antibiotic susceptibility testing from blood culture bottles with species agnostic real time polymerase chain reaction |
| url | https://doi.org/10.1371/journal.pone.0209042 |
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