Effects of <i>Auricularia auricula</i> Polysaccharides on Gut Microbiota Composition in Type 2 Diabetic Mice
In previous studies, <i>Auricularia</i><i>auricula</i> polysaccharides (AAP) has been found to improve type 2 diabetes mellitus, but its mechanism remains unclear. In this study, we sought to demonstrate that AAP achieves remission by altering the gut microbiota in mice with...
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MDPI AG
2022-09-01
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| Series: | Molecules |
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| author | Nannan Liu Mengyin Chen Juanna Song Yuanyuan Zhao Pin Gong Xuefeng Chen |
| author_facet | Nannan Liu Mengyin Chen Juanna Song Yuanyuan Zhao Pin Gong Xuefeng Chen |
| author_sort | Nannan Liu |
| collection | DOAJ |
| description | In previous studies, <i>Auricularia</i><i>auricula</i> polysaccharides (AAP) has been found to improve type 2 diabetes mellitus, but its mechanism remains unclear. In this study, we sought to demonstrate that AAP achieves remission by altering the gut microbiota in mice with type 2 diabetes. We successfully constructed a type 2 diabetes mellitus (T2DM) model induced by a high-fat diet (HFD) combined with streptozotocin (STZ), following which fasting blood glucose (FBG) levels and oral glucose tolerance test (OTGG) were observed to decrease significantly after 5 weeks of AAP intervention. Furthermore, AAP enhanced the activities of total superoxide dismutase (T-SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), and reduced the content of malondialdehyde (MDA) to alleviate the oxidative stress injury. AAP-M (200 mg/kg/d) displayed the best improvement effect. Moreover, 16S rRNA results showed that AAP decreased the abundance of Firmicutes and increased that of Bacteroidetes. The abundance of beneficial genera such as <i>Faecalibaculum, Dubosiella</i>, <i>Alloprevotella, and</i> those belonging to the family <i>Lachnospiraceae</i> was increased due to the intake of AAP. AAP could reduced the abundance of <i>Desulfovibrio, Enterorhabdus</i>, and <i>Helicobacter</i>. In all, these results suggest that AAP can improve the disorders of glucose and lipid metabolism by regulating the structure of the gut microbiota. |
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| format | Article |
| id | doaj.art-a51c77e323de456a8ba501c0025648f2 |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:00:16Z |
| publishDate | 2022-09-01 |
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| series | Molecules |
| spelling | doaj.art-a51c77e323de456a8ba501c0025648f22023-11-23T18:03:33ZengMDPI AGMolecules1420-30492022-09-012718606110.3390/molecules27186061Effects of <i>Auricularia auricula</i> Polysaccharides on Gut Microbiota Composition in Type 2 Diabetic MiceNannan Liu0Mengyin Chen1Juanna Song2Yuanyuan Zhao3Pin Gong4Xuefeng Chen5College of Chemistry and Materials Science, Weinan Normal University, Weinan 714099, ChinaSchool of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaCollege of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaSchool of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaIn previous studies, <i>Auricularia</i><i>auricula</i> polysaccharides (AAP) has been found to improve type 2 diabetes mellitus, but its mechanism remains unclear. In this study, we sought to demonstrate that AAP achieves remission by altering the gut microbiota in mice with type 2 diabetes. We successfully constructed a type 2 diabetes mellitus (T2DM) model induced by a high-fat diet (HFD) combined with streptozotocin (STZ), following which fasting blood glucose (FBG) levels and oral glucose tolerance test (OTGG) were observed to decrease significantly after 5 weeks of AAP intervention. Furthermore, AAP enhanced the activities of total superoxide dismutase (T-SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), and reduced the content of malondialdehyde (MDA) to alleviate the oxidative stress injury. AAP-M (200 mg/kg/d) displayed the best improvement effect. Moreover, 16S rRNA results showed that AAP decreased the abundance of Firmicutes and increased that of Bacteroidetes. The abundance of beneficial genera such as <i>Faecalibaculum, Dubosiella</i>, <i>Alloprevotella, and</i> those belonging to the family <i>Lachnospiraceae</i> was increased due to the intake of AAP. AAP could reduced the abundance of <i>Desulfovibrio, Enterorhabdus</i>, and <i>Helicobacter</i>. In all, these results suggest that AAP can improve the disorders of glucose and lipid metabolism by regulating the structure of the gut microbiota.https://www.mdpi.com/1420-3049/27/18/6061<i>Auricularia auricula</i> polysaccharide (AAP)hypoglycemic activitygut microbiotaantioxidant capacity |
| spellingShingle | Nannan Liu Mengyin Chen Juanna Song Yuanyuan Zhao Pin Gong Xuefeng Chen Effects of <i>Auricularia auricula</i> Polysaccharides on Gut Microbiota Composition in Type 2 Diabetic Mice Molecules <i>Auricularia auricula</i> polysaccharide (AAP) hypoglycemic activity gut microbiota antioxidant capacity |
| title | Effects of <i>Auricularia auricula</i> Polysaccharides on Gut Microbiota Composition in Type 2 Diabetic Mice |
| title_full | Effects of <i>Auricularia auricula</i> Polysaccharides on Gut Microbiota Composition in Type 2 Diabetic Mice |
| title_fullStr | Effects of <i>Auricularia auricula</i> Polysaccharides on Gut Microbiota Composition in Type 2 Diabetic Mice |
| title_full_unstemmed | Effects of <i>Auricularia auricula</i> Polysaccharides on Gut Microbiota Composition in Type 2 Diabetic Mice |
| title_short | Effects of <i>Auricularia auricula</i> Polysaccharides on Gut Microbiota Composition in Type 2 Diabetic Mice |
| title_sort | effects of i auricularia auricula i polysaccharides on gut microbiota composition in type 2 diabetic mice |
| topic | <i>Auricularia auricula</i> polysaccharide (AAP) hypoglycemic activity gut microbiota antioxidant capacity |
| url | https://www.mdpi.com/1420-3049/27/18/6061 |
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