Characterization of Cichopeptins, New Phytotoxic Cyclic Lipodepsipeptides Produced by Pseudomonas cichorii SF1-54 and Their Role in Bacterial Midrib Rot Disease of Lettuce
The lettuce midrib rot pathogen Pseudomonas cichorii SF1-54 produces seven bioactive compounds with biosurfactant properties. Two compounds exhibited necrosis-inducing activity on chicory leaves. The structure of the two phytotoxic compounds, named cichopeptin A and B, was tentatively characterized....
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2015-09-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-03-15-0061-R |
| _version_ | 1818914955948195840 |
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| author | Chien-Jui Huang Ellen Pauwelyn Marc Ongena Delphine Debois Valerie Leclère Philippe Jacques Peter Bleyaert Monica Höfte |
| author_facet | Chien-Jui Huang Ellen Pauwelyn Marc Ongena Delphine Debois Valerie Leclère Philippe Jacques Peter Bleyaert Monica Höfte |
| author_sort | Chien-Jui Huang |
| collection | DOAJ |
| description | The lettuce midrib rot pathogen Pseudomonas cichorii SF1-54 produces seven bioactive compounds with biosurfactant properties. Two compounds exhibited necrosis-inducing activity on chicory leaves. The structure of the two phytotoxic compounds, named cichopeptin A and B, was tentatively characterized. They are related cyclic lipopeptides composed of an unsaturated C12-fatty acid chain linked to the N-terminus of a 22–amino acid peptide moiety. Cichopeptin B differs from cichopeptin A only in the last C-terminal amino acid residue, which is probably Val instead of Leu/Ile. Based on peptide sequence similarity, cichopeptins are new cyclic lipopeptides related to corpeptin, produced by the tomato pathogen Pseudomonas corrugata. Production of cichopeptin is stimulated by glycine betaine but not by choline, an upstream precursor of glycine betaine. Furthermore, a gene cluster encoding cichopeptin synthethases, cipABCDEF, is responsible for cichopeptin biosynthesis. A cipA-deletion mutant exhibited significantly less virulence and rotten midribs than the parental strain upon spray inoculation on lettuce. However, the parental and mutant strains multiplied in lettuce leaves at a similar rate. These results demonstrate that cichopeptins contribute to virulence of P. cichorii SF1-54 on lettuce. |
| first_indexed | 2024-12-19T23:54:37Z |
| format | Article |
| id | doaj.art-a51dfed5fcf945099309c7e6f820987c |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:54:37Z |
| publishDate | 2015-09-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-a51dfed5fcf945099309c7e6f820987c2022-12-21T20:01:03ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062015-09-012891009102210.1094/MPMI-03-15-0061-RCharacterization of Cichopeptins, New Phytotoxic Cyclic Lipodepsipeptides Produced by Pseudomonas cichorii SF1-54 and Their Role in Bacterial Midrib Rot Disease of LettuceChien-Jui Huang0Ellen Pauwelyn1Marc Ongena2Delphine Debois3Valerie Leclère4Philippe Jacques5Peter Bleyaert6Monica Höfte7Department of Crop Protection, Laboratory of Phytopathology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium;Department of Crop Protection, Laboratory of Phytopathology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium;Walloon Centre for Industrial Biology, University of Liège–Gembloux Agro-Bio Tech, Passage des Déportés 2, 5030 Gembloux, Belgium;Mass Spectrometry Laboratory (LSM/GIGA-R), Chemistry Department, University of Liege, 4000 Liege, Belgium;Laboratoire de Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM), Université de Lille Sciences et Technologies, 59655 Villeneuve d’Ascq Cedex, FranceLaboratoire de Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM), Université de Lille Sciences et Technologies, 59655 Villeneuve d’Ascq Cedex, FranceInagro vzw, Ieperseweg 87, 8800 Rumbeke, Belgium;Department of Crop Protection, Laboratory of Phytopathology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium;The lettuce midrib rot pathogen Pseudomonas cichorii SF1-54 produces seven bioactive compounds with biosurfactant properties. Two compounds exhibited necrosis-inducing activity on chicory leaves. The structure of the two phytotoxic compounds, named cichopeptin A and B, was tentatively characterized. They are related cyclic lipopeptides composed of an unsaturated C12-fatty acid chain linked to the N-terminus of a 22–amino acid peptide moiety. Cichopeptin B differs from cichopeptin A only in the last C-terminal amino acid residue, which is probably Val instead of Leu/Ile. Based on peptide sequence similarity, cichopeptins are new cyclic lipopeptides related to corpeptin, produced by the tomato pathogen Pseudomonas corrugata. Production of cichopeptin is stimulated by glycine betaine but not by choline, an upstream precursor of glycine betaine. Furthermore, a gene cluster encoding cichopeptin synthethases, cipABCDEF, is responsible for cichopeptin biosynthesis. A cipA-deletion mutant exhibited significantly less virulence and rotten midribs than the parental strain upon spray inoculation on lettuce. However, the parental and mutant strains multiplied in lettuce leaves at a similar rate. These results demonstrate that cichopeptins contribute to virulence of P. cichorii SF1-54 on lettuce.https://apsjournals.apsnet.org/doi/10.1094/MPMI-03-15-0061-R |
| spellingShingle | Chien-Jui Huang Ellen Pauwelyn Marc Ongena Delphine Debois Valerie Leclère Philippe Jacques Peter Bleyaert Monica Höfte Characterization of Cichopeptins, New Phytotoxic Cyclic Lipodepsipeptides Produced by Pseudomonas cichorii SF1-54 and Their Role in Bacterial Midrib Rot Disease of Lettuce Molecular Plant-Microbe Interactions |
| title | Characterization of Cichopeptins, New Phytotoxic Cyclic Lipodepsipeptides Produced by Pseudomonas cichorii SF1-54 and Their Role in Bacterial Midrib Rot Disease of Lettuce |
| title_full | Characterization of Cichopeptins, New Phytotoxic Cyclic Lipodepsipeptides Produced by Pseudomonas cichorii SF1-54 and Their Role in Bacterial Midrib Rot Disease of Lettuce |
| title_fullStr | Characterization of Cichopeptins, New Phytotoxic Cyclic Lipodepsipeptides Produced by Pseudomonas cichorii SF1-54 and Their Role in Bacterial Midrib Rot Disease of Lettuce |
| title_full_unstemmed | Characterization of Cichopeptins, New Phytotoxic Cyclic Lipodepsipeptides Produced by Pseudomonas cichorii SF1-54 and Their Role in Bacterial Midrib Rot Disease of Lettuce |
| title_short | Characterization of Cichopeptins, New Phytotoxic Cyclic Lipodepsipeptides Produced by Pseudomonas cichorii SF1-54 and Their Role in Bacterial Midrib Rot Disease of Lettuce |
| title_sort | characterization of cichopeptins new phytotoxic cyclic lipodepsipeptides produced by pseudomonas cichorii sf1 54 and their role in bacterial midrib rot disease of lettuce |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-03-15-0061-R |
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