Liquid-Liquid Extraction Based Simple Trans-Resveratrol Analysis In Wine Samples
Abstract: 3,5,4-trans-trihydroxystilbene (trans-resveratrol) is quite soluble in CH2Cl2 and CHCl3. Wine samples are shaken in and rinsed with CHCl3 to obtain fine extracts of trans-resveratrol. The obtained CHCl3 solutions were directly injected to HPLC system for analysis. Determinations were made...
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| Format: | Article |
| Language: | English |
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Suleyman Demirel University
2013-06-01
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| Series: | Süleyman Demirel Üniversitesi Fen-Edebiyat Fakültesi Fen Dergisi |
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| Online Access: | http://dergipark.gov.tr/sdufeffd/issue/11277/134764?publisher=sdu-1 |
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| author | Emine İnal Sevi Öz Arda Atakol Mehmet Akay Orhan Atakol |
| author_facet | Emine İnal Sevi Öz Arda Atakol Mehmet Akay Orhan Atakol |
| author_sort | Emine İnal |
| collection | DOAJ |
| description | Abstract: 3,5,4-trans-trihydroxystilbene (trans-resveratrol) is quite soluble in CH2Cl2 and CHCl3. Wine samples are shaken in and rinsed with CHCl3 to obtain fine extracts of trans-resveratrol. The obtained CHCl3 solutions were directly injected to HPLC system for analysis. Determinations were made in 60% MeCN: 40% EtOH, and by trans-resveratrol standard addition. The resolution is calculated to be about 1.0 in the chromatogram. In 9 wine samples produced in Turkey, resveratrol concentrations were detected in between 1.46 and 6.53 ppm. By consecutive extractions, extraction ratio of resveratrol was determined and by increasing the amount of the samples, accuracy of the method was tested. It was found that the recovery is about 100.42 %.
Key words: Resveratrol determination, wine, HPLC, liquid-liquid extraction.
Şarap Örneklerinde Sıvı-Sıvı Ekstraksiyonu İle Trans-Resveratrol Analizi
Özet: 3,5,4-trans-trihidroksistilben (trans-resveratrol)'in CH2Cl2 ve CHCl3 içindeki çözünürlüğü oldukça fazladır. Trans-resveratrolü tam ekstrakte etmek için, şarap örnekleri CHCl3 içinde çalkalandı. Elde edilen CHCl3 çözeltisi HPLC sistemine direk olarak enjekte edildi. Analiz, %60 MeCN: %40 EtOH içinde ve trans-resveratrol standart eklenmesiyle gerçekleştirildi. Kromatogramdaki rezolüsyon yaklaşık 1.0 olarak hesaplandı. Türkiye de üretilmiş olan 9 şarap örneğinde resveratrol konsantrasyonu 1.46-6.53 ppm olarak bulundu. Resveratrolün ekstraksiyon oranı, ardışık ekstraksiyon ile tespit edildi ve örnek miktarı arttırılarak, metodun doğruluğu test edildi. Geri kazanım % 100.42 olarak bulundu.
Anahtar kelimeler: Resveratrol tayini, şarap, HPLC, sıvı-sıvı ekstraksiyonu. |
| first_indexed | 2024-04-10T14:36:16Z |
| format | Article |
| id | doaj.art-a51e60266efd4786a64229c87873b8cf |
| institution | Directory Open Access Journal |
| issn | 1306-7575 |
| language | English |
| last_indexed | 2024-04-10T14:36:16Z |
| publishDate | 2013-06-01 |
| publisher | Suleyman Demirel University |
| record_format | Article |
| series | Süleyman Demirel Üniversitesi Fen-Edebiyat Fakültesi Fen Dergisi |
| spelling | doaj.art-a51e60266efd4786a64229c87873b8cf2023-02-15T16:08:30ZengSuleyman Demirel UniversitySüleyman Demirel Üniversitesi Fen-Edebiyat Fakültesi Fen Dergisi1306-75752013-06-01811141211113Liquid-Liquid Extraction Based Simple Trans-Resveratrol Analysis In Wine SamplesEmine İnalSevi ÖzArda AtakolMehmet AkayOrhan AtakolAbstract: 3,5,4-trans-trihydroxystilbene (trans-resveratrol) is quite soluble in CH2Cl2 and CHCl3. Wine samples are shaken in and rinsed with CHCl3 to obtain fine extracts of trans-resveratrol. The obtained CHCl3 solutions were directly injected to HPLC system for analysis. Determinations were made in 60% MeCN: 40% EtOH, and by trans-resveratrol standard addition. The resolution is calculated to be about 1.0 in the chromatogram. In 9 wine samples produced in Turkey, resveratrol concentrations were detected in between 1.46 and 6.53 ppm. By consecutive extractions, extraction ratio of resveratrol was determined and by increasing the amount of the samples, accuracy of the method was tested. It was found that the recovery is about 100.42 %. Key words: Resveratrol determination, wine, HPLC, liquid-liquid extraction. Şarap Örneklerinde Sıvı-Sıvı Ekstraksiyonu İle Trans-Resveratrol Analizi Özet: 3,5,4-trans-trihidroksistilben (trans-resveratrol)'in CH2Cl2 ve CHCl3 içindeki çözünürlüğü oldukça fazladır. Trans-resveratrolü tam ekstrakte etmek için, şarap örnekleri CHCl3 içinde çalkalandı. Elde edilen CHCl3 çözeltisi HPLC sistemine direk olarak enjekte edildi. Analiz, %60 MeCN: %40 EtOH içinde ve trans-resveratrol standart eklenmesiyle gerçekleştirildi. Kromatogramdaki rezolüsyon yaklaşık 1.0 olarak hesaplandı. Türkiye de üretilmiş olan 9 şarap örneğinde resveratrol konsantrasyonu 1.46-6.53 ppm olarak bulundu. Resveratrolün ekstraksiyon oranı, ardışık ekstraksiyon ile tespit edildi ve örnek miktarı arttırılarak, metodun doğruluğu test edildi. Geri kazanım % 100.42 olarak bulundu. Anahtar kelimeler: Resveratrol tayini, şarap, HPLC, sıvı-sıvı ekstraksiyonu.http://dergipark.gov.tr/sdufeffd/issue/11277/134764?publisher=sdu-1-Resveratrol determination wine HPLC liquid-liquid extractionResveratrol tayini şarap HPLC sıvı-sıvı ekstraksiyonu |
| spellingShingle | Emine İnal Sevi Öz Arda Atakol Mehmet Akay Orhan Atakol Liquid-Liquid Extraction Based Simple Trans-Resveratrol Analysis In Wine Samples Süleyman Demirel Üniversitesi Fen-Edebiyat Fakültesi Fen Dergisi - Resveratrol determination wine HPLC liquid-liquid extraction Resveratrol tayini şarap HPLC sıvı-sıvı ekstraksiyonu |
| title | Liquid-Liquid Extraction Based Simple Trans-Resveratrol Analysis In Wine Samples |
| title_full | Liquid-Liquid Extraction Based Simple Trans-Resveratrol Analysis In Wine Samples |
| title_fullStr | Liquid-Liquid Extraction Based Simple Trans-Resveratrol Analysis In Wine Samples |
| title_full_unstemmed | Liquid-Liquid Extraction Based Simple Trans-Resveratrol Analysis In Wine Samples |
| title_short | Liquid-Liquid Extraction Based Simple Trans-Resveratrol Analysis In Wine Samples |
| title_sort | liquid liquid extraction based simple trans resveratrol analysis in wine samples |
| topic | - Resveratrol determination wine HPLC liquid-liquid extraction Resveratrol tayini şarap HPLC sıvı-sıvı ekstraksiyonu |
| url | http://dergipark.gov.tr/sdufeffd/issue/11277/134764?publisher=sdu-1 |
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