Engineering Saccharomyces cerevisiae for improved biofilm formation and ethanol production in continuous fermentation
Abstract Background Biofilm-immobilized continuous fermentation has the potential to enhance cellular environmental tolerance, maintain cell activity and improve production efficiency. Results In this study, different biofilm-forming genes (FLO5, FLO8 and FLO10) were integrated into the genome of S....
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-023-02356-6 |
| _version_ | 1797752851989004288 |
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| author | Zhenyu Wang Weikai Xu Yixuan Gao Mingwei Zha Di Zhang Xiwei Peng Huifang Zhang Cheng Wang Chenchen Xu Tingqiu Zhou Dong Liu Huanqing Niu Qingguo Liu Yong Chen Chenjie Zhu Ting Guo Hanjie Ying |
| author_facet | Zhenyu Wang Weikai Xu Yixuan Gao Mingwei Zha Di Zhang Xiwei Peng Huifang Zhang Cheng Wang Chenchen Xu Tingqiu Zhou Dong Liu Huanqing Niu Qingguo Liu Yong Chen Chenjie Zhu Ting Guo Hanjie Ying |
| author_sort | Zhenyu Wang |
| collection | DOAJ |
| description | Abstract Background Biofilm-immobilized continuous fermentation has the potential to enhance cellular environmental tolerance, maintain cell activity and improve production efficiency. Results In this study, different biofilm-forming genes (FLO5, FLO8 and FLO10) were integrated into the genome of S. cerevisiae for overexpression, while FLO5 and FLO10 gave the best results. The biofilm formation of the engineered strains 1308-FLO5 and 1308-FLO10 was improved by 31.3% and 58.7% compared to that of the WT strain, respectively. The counts of cells adhering onto the biofilm carrier were increased. Compared to free-cell fermentation, the average ethanol production of 1308, 1308-FLO5 and 1308-FLO10 was increased by 17.4%, 20.8% and 19.1% in the biofilm-immobilized continuous fermentation, respectively. Due to good adhering ability, the fermentation broth turbidity of 1308-FLO5 and 1308-FLO10 was decreased by 22.3% and 59.1% in the biofilm-immobilized fermentation, respectively. Subsequently, for biofilm-immobilized fermentation coupled with membrane separation, the engineered strain significantly reduced the pollution of cells onto the membrane and the membrane separation flux was increased by 36.3%. Conclusions In conclusion, enhanced biofilm-forming capability of S. cerevisiae could offer multiple benefits in ethanol fermentation. Graphical Abstract |
| first_indexed | 2024-03-12T17:09:27Z |
| format | Article |
| id | doaj.art-a520eab478c64c56b50ec8478f1bfd55 |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-03-12T17:09:27Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-a520eab478c64c56b50ec8478f1bfd552023-08-06T11:09:31ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542023-07-0116111010.1186/s13068-023-02356-6Engineering Saccharomyces cerevisiae for improved biofilm formation and ethanol production in continuous fermentationZhenyu Wang0Weikai Xu1Yixuan Gao2Mingwei Zha3Di Zhang4Xiwei Peng5Huifang Zhang6Cheng Wang7Chenchen Xu8Tingqiu Zhou9Dong Liu10Huanqing Niu11Qingguo Liu12Yong Chen13Chenjie Zhu14Ting Guo15Hanjie Ying16State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityInstitute of Industrial Biotechnology, Jiangsu Industrial Technology Research Institute (JITRI)State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityJiangsu Academy of Agricultural SciencesState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech UniversityAbstract Background Biofilm-immobilized continuous fermentation has the potential to enhance cellular environmental tolerance, maintain cell activity and improve production efficiency. Results In this study, different biofilm-forming genes (FLO5, FLO8 and FLO10) were integrated into the genome of S. cerevisiae for overexpression, while FLO5 and FLO10 gave the best results. The biofilm formation of the engineered strains 1308-FLO5 and 1308-FLO10 was improved by 31.3% and 58.7% compared to that of the WT strain, respectively. The counts of cells adhering onto the biofilm carrier were increased. Compared to free-cell fermentation, the average ethanol production of 1308, 1308-FLO5 and 1308-FLO10 was increased by 17.4%, 20.8% and 19.1% in the biofilm-immobilized continuous fermentation, respectively. Due to good adhering ability, the fermentation broth turbidity of 1308-FLO5 and 1308-FLO10 was decreased by 22.3% and 59.1% in the biofilm-immobilized fermentation, respectively. Subsequently, for biofilm-immobilized fermentation coupled with membrane separation, the engineered strain significantly reduced the pollution of cells onto the membrane and the membrane separation flux was increased by 36.3%. Conclusions In conclusion, enhanced biofilm-forming capability of S. cerevisiae could offer multiple benefits in ethanol fermentation. Graphical Abstracthttps://doi.org/10.1186/s13068-023-02356-6BiofilmEthanolContinuous fermentationMembrane separation |
| spellingShingle | Zhenyu Wang Weikai Xu Yixuan Gao Mingwei Zha Di Zhang Xiwei Peng Huifang Zhang Cheng Wang Chenchen Xu Tingqiu Zhou Dong Liu Huanqing Niu Qingguo Liu Yong Chen Chenjie Zhu Ting Guo Hanjie Ying Engineering Saccharomyces cerevisiae for improved biofilm formation and ethanol production in continuous fermentation Biotechnology for Biofuels and Bioproducts Biofilm Ethanol Continuous fermentation Membrane separation |
| title | Engineering Saccharomyces cerevisiae for improved biofilm formation and ethanol production in continuous fermentation |
| title_full | Engineering Saccharomyces cerevisiae for improved biofilm formation and ethanol production in continuous fermentation |
| title_fullStr | Engineering Saccharomyces cerevisiae for improved biofilm formation and ethanol production in continuous fermentation |
| title_full_unstemmed | Engineering Saccharomyces cerevisiae for improved biofilm formation and ethanol production in continuous fermentation |
| title_short | Engineering Saccharomyces cerevisiae for improved biofilm formation and ethanol production in continuous fermentation |
| title_sort | engineering saccharomyces cerevisiae for improved biofilm formation and ethanol production in continuous fermentation |
| topic | Biofilm Ethanol Continuous fermentation Membrane separation |
| url | https://doi.org/10.1186/s13068-023-02356-6 |
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