Saikosaponin D attenuates inflammatory response and cell apoptosis of lipopolysaccharide‐induced lung epithelial cells
Abstract Background Acute lung injury (ALI) is a prevalent complication of sepsis with high mortality rate. Saikosaponin D (SSD) is a triterpenoid saponin that has been reported to alleviate sepsis‐triggered renal injury in mice. Nonetheless, the therapeutic effect of SSD on sepsis‐evoked ALI is unc...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-10-01
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| Series: | The Clinical Respiratory Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/crj.13688 |
| _version_ | 1797668920240373760 |
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| author | Lijie Song Guoyu Lu Yanyan Tao |
| author_facet | Lijie Song Guoyu Lu Yanyan Tao |
| author_sort | Lijie Song |
| collection | DOAJ |
| description | Abstract Background Acute lung injury (ALI) is a prevalent complication of sepsis with high mortality rate. Saikosaponin D (SSD) is a triterpenoid saponin that has been reported to alleviate sepsis‐triggered renal injury in mice. Nonetheless, the therapeutic effect of SSD on sepsis‐evoked ALI is unclarified. Methods Lipopolysaccharide (LPS) from Escherichia coli 055:B5 was utilized to stimulate lung epithelial cell line MLE‐12. A mouse model of sepsis was established. CCK‐8 assay was employed for determining cytotoxicity. ELISA was utilized for determining proinflammatory cytokine production. Flow cytometry and western blotting were implemented for evaluating cell apoptosis. Hematoxylin–eosin staining was conducted for histologic analysis of murine lung tissues. Results SSD alleviated LPS‐triggered inflammation and cell apoptosis of MLE‐12 cells. SSD treatment ameliorated the pathological damages, inflammatory response, and cell apoptosis in the lungs of septic mice. Conclusion SSD protects against sepsis‐triggered ALI by inhibiting inflammation and cell apoptosis in MLE‐12 cells and septic mouse mice. |
| first_indexed | 2024-03-11T20:36:33Z |
| format | Article |
| id | doaj.art-a5231034a4fd46e49816221a847c1820 |
| institution | Directory Open Access Journal |
| issn | 1752-6981 1752-699X |
| language | English |
| last_indexed | 2024-03-11T20:36:33Z |
| publishDate | 2023-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Clinical Respiratory Journal |
| spelling | doaj.art-a5231034a4fd46e49816221a847c18202023-10-02T06:33:21ZengWileyThe Clinical Respiratory Journal1752-69811752-699X2023-10-0117101017102410.1111/crj.13688Saikosaponin D attenuates inflammatory response and cell apoptosis of lipopolysaccharide‐induced lung epithelial cellsLijie Song0Guoyu Lu1Yanyan Tao2Department of Emergency medical The First Affiliated Hospital of Bengbu Medical College Bengbu ChinaDepartment of Emergency medical The First Affiliated Hospital of Bengbu Medical College Bengbu ChinaDepartment of Emergency medical The First Affiliated Hospital of Bengbu Medical College Bengbu ChinaAbstract Background Acute lung injury (ALI) is a prevalent complication of sepsis with high mortality rate. Saikosaponin D (SSD) is a triterpenoid saponin that has been reported to alleviate sepsis‐triggered renal injury in mice. Nonetheless, the therapeutic effect of SSD on sepsis‐evoked ALI is unclarified. Methods Lipopolysaccharide (LPS) from Escherichia coli 055:B5 was utilized to stimulate lung epithelial cell line MLE‐12. A mouse model of sepsis was established. CCK‐8 assay was employed for determining cytotoxicity. ELISA was utilized for determining proinflammatory cytokine production. Flow cytometry and western blotting were implemented for evaluating cell apoptosis. Hematoxylin–eosin staining was conducted for histologic analysis of murine lung tissues. Results SSD alleviated LPS‐triggered inflammation and cell apoptosis of MLE‐12 cells. SSD treatment ameliorated the pathological damages, inflammatory response, and cell apoptosis in the lungs of septic mice. Conclusion SSD protects against sepsis‐triggered ALI by inhibiting inflammation and cell apoptosis in MLE‐12 cells and septic mouse mice.https://doi.org/10.1111/crj.13688acute lung injuryapoptosisinflammationSaikosaponin Dsepsis |
| spellingShingle | Lijie Song Guoyu Lu Yanyan Tao Saikosaponin D attenuates inflammatory response and cell apoptosis of lipopolysaccharide‐induced lung epithelial cells The Clinical Respiratory Journal acute lung injury apoptosis inflammation Saikosaponin D sepsis |
| title | Saikosaponin D attenuates inflammatory response and cell apoptosis of lipopolysaccharide‐induced lung epithelial cells |
| title_full | Saikosaponin D attenuates inflammatory response and cell apoptosis of lipopolysaccharide‐induced lung epithelial cells |
| title_fullStr | Saikosaponin D attenuates inflammatory response and cell apoptosis of lipopolysaccharide‐induced lung epithelial cells |
| title_full_unstemmed | Saikosaponin D attenuates inflammatory response and cell apoptosis of lipopolysaccharide‐induced lung epithelial cells |
| title_short | Saikosaponin D attenuates inflammatory response and cell apoptosis of lipopolysaccharide‐induced lung epithelial cells |
| title_sort | saikosaponin d attenuates inflammatory response and cell apoptosis of lipopolysaccharide induced lung epithelial cells |
| topic | acute lung injury apoptosis inflammation Saikosaponin D sepsis |
| url | https://doi.org/10.1111/crj.13688 |
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