Morphological and Molecular Identification of <i>Dactylogyrus gobiocypris</i> (Monogenea: Dactylogyridae) on Gills of a Model Fish, <i>Gobiocypris rarus</i> (Cypriniformes: Gobionidae)
The rare minnow <i>Gobiocypris rarus</i> is an ideal model organism for toxicological research. <i>Dactylogyrus</i> species are usually found on the gills of this rare minnow in laboratory farming systems. Dactylogyrid infection may change the sensibility of fish to toxicants...
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MDPI AG
2023-01-01
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| author | Jiangwen Cheng Hong Zou Ming Li Jianwei Wang Guitang Wang Wenxiang Li |
| author_facet | Jiangwen Cheng Hong Zou Ming Li Jianwei Wang Guitang Wang Wenxiang Li |
| author_sort | Jiangwen Cheng |
| collection | DOAJ |
| description | The rare minnow <i>Gobiocypris rarus</i> is an ideal model organism for toxicological research. <i>Dactylogyrus</i> species are usually found on the gills of this rare minnow in laboratory farming systems. Dactylogyrid infection may change the sensibility of fish to toxicants and affect toxicological evaluations. In the present study, dactylogyrid infection was investigated, and species of <i>Dactylogyrus</i> collected from rare minnows were determined. Based on the observed ‘<i>D. wunderi</i>’ type anchors, with a shorter outer root and elongated inner root, and accessory piece consisting of two parts, the dactylogyrids were identified as <i>D. gobiocypris</i>. A partial 18S-ITS1 rDNA sequence was firstly sequenced, and the highest sequence identity (86.7%) was to <i>D. cryptomeres</i>. Phylogenetic analysis revealed that <i>D. gobiocypris</i> formed a clade with <i>D. squameus</i>, <i>D. finitimus</i>, and <i>D. cryptomeres</i>, all of which have been recorded in the family Gobionidae. Histopathology analysis indicated that a heavy burden of <i>D. gobiocypris</i> caused necrosis of gill filaments. Inflammatory responses, such as tumefaction and hyperaemia, were also observed on gills with severe dactylogyrid infection. Supplementary morphological characteristics and 18S-ITS1 rDNA sequence provided basic data for identification of this parasite species. |
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| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-11T08:19:08Z |
| publishDate | 2023-01-01 |
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| series | Pathogens |
| spelling | doaj.art-a523f194aa274992a3fd825bea95f7432023-11-16T22:33:29ZengMDPI AGPathogens2076-08172023-01-0112220610.3390/pathogens12020206Morphological and Molecular Identification of <i>Dactylogyrus gobiocypris</i> (Monogenea: Dactylogyridae) on Gills of a Model Fish, <i>Gobiocypris rarus</i> (Cypriniformes: Gobionidae)Jiangwen Cheng0Hong Zou1Ming Li2Jianwei Wang3Guitang Wang4Wenxiang Li5State Key Laboratory of Freshwater Ecology and Biotechnology and Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology and Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology and Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology and Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology and Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology and Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaThe rare minnow <i>Gobiocypris rarus</i> is an ideal model organism for toxicological research. <i>Dactylogyrus</i> species are usually found on the gills of this rare minnow in laboratory farming systems. Dactylogyrid infection may change the sensibility of fish to toxicants and affect toxicological evaluations. In the present study, dactylogyrid infection was investigated, and species of <i>Dactylogyrus</i> collected from rare minnows were determined. Based on the observed ‘<i>D. wunderi</i>’ type anchors, with a shorter outer root and elongated inner root, and accessory piece consisting of two parts, the dactylogyrids were identified as <i>D. gobiocypris</i>. A partial 18S-ITS1 rDNA sequence was firstly sequenced, and the highest sequence identity (86.7%) was to <i>D. cryptomeres</i>. Phylogenetic analysis revealed that <i>D. gobiocypris</i> formed a clade with <i>D. squameus</i>, <i>D. finitimus</i>, and <i>D. cryptomeres</i>, all of which have been recorded in the family Gobionidae. Histopathology analysis indicated that a heavy burden of <i>D. gobiocypris</i> caused necrosis of gill filaments. Inflammatory responses, such as tumefaction and hyperaemia, were also observed on gills with severe dactylogyrid infection. Supplementary morphological characteristics and 18S-ITS1 rDNA sequence provided basic data for identification of this parasite species.https://www.mdpi.com/2076-0817/12/2/206dactylogyrid infectionrare minnow<i>Gobiocypris rarus</i><i>Dactylogyrus gobiocypris</i>histopathology |
| spellingShingle | Jiangwen Cheng Hong Zou Ming Li Jianwei Wang Guitang Wang Wenxiang Li Morphological and Molecular Identification of <i>Dactylogyrus gobiocypris</i> (Monogenea: Dactylogyridae) on Gills of a Model Fish, <i>Gobiocypris rarus</i> (Cypriniformes: Gobionidae) Pathogens dactylogyrid infection rare minnow <i>Gobiocypris rarus</i> <i>Dactylogyrus gobiocypris</i> histopathology |
| title | Morphological and Molecular Identification of <i>Dactylogyrus gobiocypris</i> (Monogenea: Dactylogyridae) on Gills of a Model Fish, <i>Gobiocypris rarus</i> (Cypriniformes: Gobionidae) |
| title_full | Morphological and Molecular Identification of <i>Dactylogyrus gobiocypris</i> (Monogenea: Dactylogyridae) on Gills of a Model Fish, <i>Gobiocypris rarus</i> (Cypriniformes: Gobionidae) |
| title_fullStr | Morphological and Molecular Identification of <i>Dactylogyrus gobiocypris</i> (Monogenea: Dactylogyridae) on Gills of a Model Fish, <i>Gobiocypris rarus</i> (Cypriniformes: Gobionidae) |
| title_full_unstemmed | Morphological and Molecular Identification of <i>Dactylogyrus gobiocypris</i> (Monogenea: Dactylogyridae) on Gills of a Model Fish, <i>Gobiocypris rarus</i> (Cypriniformes: Gobionidae) |
| title_short | Morphological and Molecular Identification of <i>Dactylogyrus gobiocypris</i> (Monogenea: Dactylogyridae) on Gills of a Model Fish, <i>Gobiocypris rarus</i> (Cypriniformes: Gobionidae) |
| title_sort | morphological and molecular identification of i dactylogyrus gobiocypris i monogenea dactylogyridae on gills of a model fish i gobiocypris rarus i cypriniformes gobionidae |
| topic | dactylogyrid infection rare minnow <i>Gobiocypris rarus</i> <i>Dactylogyrus gobiocypris</i> histopathology |
| url | https://www.mdpi.com/2076-0817/12/2/206 |
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