A prospective study to evaluate the gender prediction of blastocysts by using cell-free DNA within a culture medium
Abstract Background: Preimplantation genetic diagnosis (PGD) has been used as an option for couples with the possibility of having a baby with a genetic disorder. The common method for performing this test involves isolating 1 cell from day 3 or a few cells from day 5 embryos and performing genetic...
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| Format: | Article |
| Language: | English |
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Shahid Sadoughi University of Medical Sciences
2022-07-01
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| Series: | International Journal of Reproductive BioMedicine |
| Subjects: | |
| Online Access: | https://doi.org/10.18502/ijrm.v20i7.11558 |
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| author | Somayeh Barzanouni Farideh Moramezi Mahvash Zargar Hamid Galehdari Masoud Hemadi |
| author_facet | Somayeh Barzanouni Farideh Moramezi Mahvash Zargar Hamid Galehdari Masoud Hemadi |
| author_sort | Somayeh Barzanouni |
| collection | DOAJ |
| description | Abstract Background: Preimplantation genetic diagnosis (PGD) has been used as an option for couples with the possibility of having a baby with a genetic disorder. The common method for performing this test involves isolating 1 cell from day 3 or a few cells from day 5 embryos and performing genetic studies on the cell-extracted DNA. This method is invasive and can cause abortion after implantation in the uterus. Because of this, 2 noninvasive methods for performing a PGD have been studied: PGD using blastocyst fluid and PGD using embryo culture medium. Objective: The aim of this study is to determine the sensitivity of the polymerase chain reaction (PCR) technique to detect the Y chromosome using cell-free DNA within a culture medium for gender prediction of blastocysts. Materials and Methods: In this study, the gender of 30 embryos on day 5 was determined using embryonic DNA extraction from the culture medium and the PCR technique to evaluate the sex-determining region Y and fragile X mental retardation genes. Then, the accuracy was assessed using ultrasound. Results: The results of the PCR technique showed that 7 embryos were male, but an ultrasound revealed that 13 were male. Conclusion: The given results indicated that, because of the low amount of DNA extracted from the culture medium, the diagnosis of the existence of the Y chromosome by this method is still not accurate enough for detecting the gender of the embryo. |
| first_indexed | 2024-04-11T11:50:03Z |
| format | Article |
| id | doaj.art-a523fdc0e21e4d7da53ba1a2c3250f95 |
| institution | Directory Open Access Journal |
| issn | 2476-4108 2476-3772 |
| language | English |
| last_indexed | 2024-04-11T11:50:03Z |
| publishDate | 2022-07-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj.art-a523fdc0e21e4d7da53ba1a2c3250f952022-12-22T04:25:24ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-41082476-37722022-07-0118756156810.18502/ijrm.v20i7.11558ijrm.v20i7.11558A prospective study to evaluate the gender prediction of blastocysts by using cell-free DNA within a culture mediumSomayeh Barzanouni0Farideh Moramezi1Mahvash Zargar2Hamid Galehdari3Masoud Hemadi4 Department of Obstetrics and Gynecology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Department of Obstetrics and Gynecology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Department of Obstetrics and Gynecology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Department of Genetics, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran. Fertility, Infertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Abstract Background: Preimplantation genetic diagnosis (PGD) has been used as an option for couples with the possibility of having a baby with a genetic disorder. The common method for performing this test involves isolating 1 cell from day 3 or a few cells from day 5 embryos and performing genetic studies on the cell-extracted DNA. This method is invasive and can cause abortion after implantation in the uterus. Because of this, 2 noninvasive methods for performing a PGD have been studied: PGD using blastocyst fluid and PGD using embryo culture medium. Objective: The aim of this study is to determine the sensitivity of the polymerase chain reaction (PCR) technique to detect the Y chromosome using cell-free DNA within a culture medium for gender prediction of blastocysts. Materials and Methods: In this study, the gender of 30 embryos on day 5 was determined using embryonic DNA extraction from the culture medium and the PCR technique to evaluate the sex-determining region Y and fragile X mental retardation genes. Then, the accuracy was assessed using ultrasound. Results: The results of the PCR technique showed that 7 embryos were male, but an ultrasound revealed that 13 were male. Conclusion: The given results indicated that, because of the low amount of DNA extracted from the culture medium, the diagnosis of the existence of the Y chromosome by this method is still not accurate enough for detecting the gender of the embryo.https://doi.org/10.18502/ijrm.v20i7.11558preimplantation diagnosis, embryo implantation, culture media, blastocyst, polymerase chain reaction. |
| spellingShingle | Somayeh Barzanouni Farideh Moramezi Mahvash Zargar Hamid Galehdari Masoud Hemadi A prospective study to evaluate the gender prediction of blastocysts by using cell-free DNA within a culture medium International Journal of Reproductive BioMedicine preimplantation diagnosis, embryo implantation, culture media, blastocyst, polymerase chain reaction. |
| title | A prospective study to evaluate the gender prediction of blastocysts by using cell-free DNA within a culture medium |
| title_full | A prospective study to evaluate the gender prediction of blastocysts by using cell-free DNA within a culture medium |
| title_fullStr | A prospective study to evaluate the gender prediction of blastocysts by using cell-free DNA within a culture medium |
| title_full_unstemmed | A prospective study to evaluate the gender prediction of blastocysts by using cell-free DNA within a culture medium |
| title_short | A prospective study to evaluate the gender prediction of blastocysts by using cell-free DNA within a culture medium |
| title_sort | prospective study to evaluate the gender prediction of blastocysts by using cell free dna within a culture medium |
| topic | preimplantation diagnosis, embryo implantation, culture media, blastocyst, polymerase chain reaction. |
| url | https://doi.org/10.18502/ijrm.v20i7.11558 |
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