Engineering Vibrio sp. SP1 for the production of carotenoids directly from brown macroalgae
Macroalgae is regarded as a promising third-generation marine biomass that can be utilized as a sustainable feedstock for bio-industry due to the high sugar level and absence of lignin. Alginate, composed of 1,4-linked D-mannuronate (M) and L-guluronate (G), is one of the major carbohydrates in brow...
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| Format: | Article |
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Elsevier
2021-01-01
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| Series: | Computational and Structural Biotechnology Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2001037021000799 |
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| author | Sungwoo Park Sung Won Cho Yungyu Lee Mincheol Choi Jina Yang Hojun Lee Sang Woo Seo |
| author_facet | Sungwoo Park Sung Won Cho Yungyu Lee Mincheol Choi Jina Yang Hojun Lee Sang Woo Seo |
| author_sort | Sungwoo Park |
| collection | DOAJ |
| description | Macroalgae is regarded as a promising third-generation marine biomass that can be utilized as a sustainable feedstock for bio-industry due to the high sugar level and absence of lignin. Alginate, composed of 1,4-linked D-mannuronate (M) and L-guluronate (G), is one of the major carbohydrates in brown macroalgae. It is difficult to be assimilated by most industrial microorganisms. Therefore, developing engineered microorganisms that can utilize alginate as a feedstock in order to produce natural products from marine biomass is critical. In this study, we isolated, characterized, and sequenced Vibrio sp. SP1 which rapidly grows using alginate as a sole carbon source. We further engineered this strain by introducing genes encoding enzymes under the control of synthetic expression cassettes to produce lycopene and β-carotene which are attractive phytochemicals owing to the antioxidant property. We confirmed that the engineered Vibrio sp. SP1 could successfully produce 2.13 ± 0.37 mg L−1 of lycopene, 2.98 ± 0.43 mg L−1 of β -carotene, respectively, from 10 g L−1 of alginate as a sole carbon source. Furthermore, our engineered strain could directly convert 60 g L−1 of brown macroalgae Sargassum fusiforme into 1.23 ± 0.21 mg L−1 of lycopene without any pretreatment which had been vitally required for the previous productions. As the first demonstrated strain to produce high-value product from Sargassum, Vibrio sp. SP1 is evaluated to be a desirable platform for the brown macroalgae-based biorefinery. |
| first_indexed | 2024-12-19T12:29:43Z |
| format | Article |
| id | doaj.art-a5272f3e40ea4050a24c64bc3408cae6 |
| institution | Directory Open Access Journal |
| issn | 2001-0370 |
| language | English |
| last_indexed | 2024-12-19T12:29:43Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Computational and Structural Biotechnology Journal |
| spelling | doaj.art-a5272f3e40ea4050a24c64bc3408cae62022-12-21T20:21:26ZengElsevierComputational and Structural Biotechnology Journal2001-03702021-01-011915311540Engineering Vibrio sp. SP1 for the production of carotenoids directly from brown macroalgaeSungwoo Park0Sung Won Cho1Yungyu Lee2Mincheol Choi3Jina Yang4Hojun Lee5Sang Woo Seo6School of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, South KoreaSchool of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, South KoreaSchool of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, South KoreaSchool of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, South KoreaSchool of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, South Korea; Institute of Chemical Processes, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, South KoreaInterdisciplinary Program in Bioengineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, South KoreaSchool of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, South Korea; Institute of Chemical Processes, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, South Korea; Interdisciplinary Program in Bioengineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, South Korea; Bio-MAX Institute Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, South Korea; Institute of Engineering Research, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, South Korea; Corresponding author at: School of Chemical and Biological Engineering, Institute of Chemical Process, Seoul National University, 1 Gwanak-Ro, Gwanak-Gu, Seoul 08826, South Korea.Macroalgae is regarded as a promising third-generation marine biomass that can be utilized as a sustainable feedstock for bio-industry due to the high sugar level and absence of lignin. Alginate, composed of 1,4-linked D-mannuronate (M) and L-guluronate (G), is one of the major carbohydrates in brown macroalgae. It is difficult to be assimilated by most industrial microorganisms. Therefore, developing engineered microorganisms that can utilize alginate as a feedstock in order to produce natural products from marine biomass is critical. In this study, we isolated, characterized, and sequenced Vibrio sp. SP1 which rapidly grows using alginate as a sole carbon source. We further engineered this strain by introducing genes encoding enzymes under the control of synthetic expression cassettes to produce lycopene and β-carotene which are attractive phytochemicals owing to the antioxidant property. We confirmed that the engineered Vibrio sp. SP1 could successfully produce 2.13 ± 0.37 mg L−1 of lycopene, 2.98 ± 0.43 mg L−1 of β -carotene, respectively, from 10 g L−1 of alginate as a sole carbon source. Furthermore, our engineered strain could directly convert 60 g L−1 of brown macroalgae Sargassum fusiforme into 1.23 ± 0.21 mg L−1 of lycopene without any pretreatment which had been vitally required for the previous productions. As the first demonstrated strain to produce high-value product from Sargassum, Vibrio sp. SP1 is evaluated to be a desirable platform for the brown macroalgae-based biorefinery.http://www.sciencedirect.com/science/article/pii/S2001037021000799MacroalgaeBiorefineryAlginateVibrio sp. SP1CarotenoidsGenome sequencing |
| spellingShingle | Sungwoo Park Sung Won Cho Yungyu Lee Mincheol Choi Jina Yang Hojun Lee Sang Woo Seo Engineering Vibrio sp. SP1 for the production of carotenoids directly from brown macroalgae Computational and Structural Biotechnology Journal Macroalgae Biorefinery Alginate Vibrio sp. SP1 Carotenoids Genome sequencing |
| title | Engineering Vibrio sp. SP1 for the production of carotenoids directly from brown macroalgae |
| title_full | Engineering Vibrio sp. SP1 for the production of carotenoids directly from brown macroalgae |
| title_fullStr | Engineering Vibrio sp. SP1 for the production of carotenoids directly from brown macroalgae |
| title_full_unstemmed | Engineering Vibrio sp. SP1 for the production of carotenoids directly from brown macroalgae |
| title_short | Engineering Vibrio sp. SP1 for the production of carotenoids directly from brown macroalgae |
| title_sort | engineering vibrio sp sp1 for the production of carotenoids directly from brown macroalgae |
| topic | Macroalgae Biorefinery Alginate Vibrio sp. SP1 Carotenoids Genome sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2001037021000799 |
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