Bioassay-Guided Isolation and Identification of Xanthine Oxidase Inhibitory Constituents from the Leaves of Perilla frutescens
Activity-directed fractionation and purification processes were employed to identify xanthine oxidase (XO) inhibitory compounds from the leaves of Perilla frutescens. The total extract was evaluated in vitro on XO inhibitory activity and in vivo in an experimental model with potassium oxonate-induce...
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MDPI AG
2015-09-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/20/10/17848 |
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| author | Li-Na Huo Wei Wang Chun-Yu Zhang Hai-Bo Shi Yang Liu Xiao-Hong Liu Bing-Hua Guo Dong-Mei Zhao Hua Gao |
| author_facet | Li-Na Huo Wei Wang Chun-Yu Zhang Hai-Bo Shi Yang Liu Xiao-Hong Liu Bing-Hua Guo Dong-Mei Zhao Hua Gao |
| author_sort | Li-Na Huo |
| collection | DOAJ |
| description | Activity-directed fractionation and purification processes were employed to identify xanthine oxidase (XO) inhibitory compounds from the leaves of Perilla frutescens. The total extract was evaluated in vitro on XO inhibitory activity and in vivo in an experimental model with potassium oxonate-induced hyperuricemia in mice which was used to evaluate anti-hyperuricemic activity. The crude extract showed expressive urate-lowering activity results. Solvent partitioning of the total extract followed by macroporous resin column chromatography of the n-butanol extract yielded four extracts and eluted parts. Among them, only the 70% ethanol eluted part of the n-butanol extract showed strong activity and therefore was subjected to separation and purification using various chromatographic techniques. Five compounds showing potent activity were identified by comparing their spectral data with literature values to be caffeic acid, vinyl caffeate, rosmarinic acid, methyl rosmarinate, and apigenin. These results indicate that pending further study, these compounds could be used as novel natural product agents for the treatment of hyperuricemia. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-14T08:23:28Z |
| publishDate | 2015-09-01 |
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| series | Molecules |
| spelling | doaj.art-a528a396577c42369a0646d08baa9f8f2022-12-22T02:04:09ZengMDPI AGMolecules1420-30492015-09-012010178481785910.3390/molecules201017848molecules201017848Bioassay-Guided Isolation and Identification of Xanthine Oxidase Inhibitory Constituents from the Leaves of Perilla frutescensLi-Na Huo0Wei Wang1Chun-Yu Zhang2Hai-Bo Shi3Yang Liu4Xiao-Hong Liu5Bing-Hua Guo6Dong-Mei Zhao7Hua Gao8College of Pharmacy, Qingdao University, Qingdao 266021, Shandong, ChinaCollege of Pharmacy, Qingdao University, Qingdao 266021, Shandong, ChinaHealth Education Institute of Changchun, Changchun 130021, Jilin, ChinaInstitute of Changbai Mountain Resources, Jilin Academy of Chinese Medicine Sciences, Changchun 130012, Jilin, ChinaCollege of Pharmacy, Qingdao University, Qingdao 266021, Shandong, ChinaCollege of Pharmacy, Qingdao University, Qingdao 266021, Shandong, ChinaCollege of Pharmacy, Qingdao University, Qingdao 266021, Shandong, ChinaCollege of Pharmacy, Qingdao University, Qingdao 266021, Shandong, ChinaCollege of Pharmacy, Qingdao University, Qingdao 266021, Shandong, ChinaActivity-directed fractionation and purification processes were employed to identify xanthine oxidase (XO) inhibitory compounds from the leaves of Perilla frutescens. The total extract was evaluated in vitro on XO inhibitory activity and in vivo in an experimental model with potassium oxonate-induced hyperuricemia in mice which was used to evaluate anti-hyperuricemic activity. The crude extract showed expressive urate-lowering activity results. Solvent partitioning of the total extract followed by macroporous resin column chromatography of the n-butanol extract yielded four extracts and eluted parts. Among them, only the 70% ethanol eluted part of the n-butanol extract showed strong activity and therefore was subjected to separation and purification using various chromatographic techniques. Five compounds showing potent activity were identified by comparing their spectral data with literature values to be caffeic acid, vinyl caffeate, rosmarinic acid, methyl rosmarinate, and apigenin. These results indicate that pending further study, these compounds could be used as novel natural product agents for the treatment of hyperuricemia.http://www.mdpi.com/1420-3049/20/10/17848Perilla frutescens (L.) Britt.hyperuricemiaxanthine oxidasepolyphenols |
| spellingShingle | Li-Na Huo Wei Wang Chun-Yu Zhang Hai-Bo Shi Yang Liu Xiao-Hong Liu Bing-Hua Guo Dong-Mei Zhao Hua Gao Bioassay-Guided Isolation and Identification of Xanthine Oxidase Inhibitory Constituents from the Leaves of Perilla frutescens Molecules Perilla frutescens (L.) Britt. hyperuricemia xanthine oxidase polyphenols |
| title | Bioassay-Guided Isolation and Identification of Xanthine Oxidase Inhibitory Constituents from the Leaves of Perilla frutescens |
| title_full | Bioassay-Guided Isolation and Identification of Xanthine Oxidase Inhibitory Constituents from the Leaves of Perilla frutescens |
| title_fullStr | Bioassay-Guided Isolation and Identification of Xanthine Oxidase Inhibitory Constituents from the Leaves of Perilla frutescens |
| title_full_unstemmed | Bioassay-Guided Isolation and Identification of Xanthine Oxidase Inhibitory Constituents from the Leaves of Perilla frutescens |
| title_short | Bioassay-Guided Isolation and Identification of Xanthine Oxidase Inhibitory Constituents from the Leaves of Perilla frutescens |
| title_sort | bioassay guided isolation and identification of xanthine oxidase inhibitory constituents from the leaves of perilla frutescens |
| topic | Perilla frutescens (L.) Britt. hyperuricemia xanthine oxidase polyphenols |
| url | http://www.mdpi.com/1420-3049/20/10/17848 |
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