Evaluation of a New Porcine Bone Graft on the Repair of Surgically Created Critical Bone Defects in Rat Calvaria: Histomorphometric and Microtomographic Study
The aim of this study was to evaluate the use of a new porcine bone graft in rat calvaria bone defects. Critical defects were surgically created in 24 rats that were divided into four experimental groups according to defect filling (<i>n</i> = 6): Control Group (CG)—blood clot; Porcine B...
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MDPI AG
2022-08-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/13/3/124 |
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| author | Ytalo Fernandes Rafael Mantovani Danilo Reino Arthur Novaes Michel Messora Luiz Gustavo Sousa Daniela Palioto Sergio Scombatti de Souza |
| author_facet | Ytalo Fernandes Rafael Mantovani Danilo Reino Arthur Novaes Michel Messora Luiz Gustavo Sousa Daniela Palioto Sergio Scombatti de Souza |
| author_sort | Ytalo Fernandes |
| collection | DOAJ |
| description | The aim of this study was to evaluate the use of a new porcine bone graft in rat calvaria bone defects. Critical defects were surgically created in 24 rats that were divided into four experimental groups according to defect filling (<i>n</i> = 6): Control Group (CG)—blood clot; Porcine Bone Group (PG)—porcine-derived bone substitute; (BG): Bio-Oss Group (BG)–chemically and heat-treated bovine graft; Bonefill Group (BFG)—chemically treated bovine bone substitute. Euthanasia of the animals occurred 30 days after the surgery, and the area of the original surgical defect and the surrounding tissues were removed for micro-CT and histomorphometric analysis. In the micro-CT evaluation, the PG presented statistically significant differences (<i>p</i> < 0.05) in comparison to the CG, BG and BFG, for the parameters percentage of Bone Volume (BV/TV), Surface Bone Density (BS/TV), Number of Trabeculae (Tb.N) and Bone Connectivity (Conn), but not for Total Porosity (Po.tot) and Trabecular Thickness (Tb.Th). The histomorphometric analysis showed that the PG presented similar results to the BG regarding newly formed bone extension and to the BG and BFG regarding newly formed bone area. The porcine-derived graft presented superior microtomographic and histomorphometric results when compared to the two bovine bone substitutes. |
| first_indexed | 2024-03-09T23:34:20Z |
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| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-09T23:34:20Z |
| publishDate | 2022-08-01 |
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| series | Journal of Functional Biomaterials |
| spelling | doaj.art-a52a509706da46bea0a6a2c1d1d9cd2b2023-11-23T17:03:26ZengMDPI AGJournal of Functional Biomaterials2079-49832022-08-0113312410.3390/jfb13030124Evaluation of a New Porcine Bone Graft on the Repair of Surgically Created Critical Bone Defects in Rat Calvaria: Histomorphometric and Microtomographic StudyYtalo Fernandes0Rafael Mantovani1Danilo Reino2Arthur Novaes3Michel Messora4Luiz Gustavo Sousa5Daniela Palioto6Sergio Scombatti de Souza7Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Av do Café, s/n., Ribeirao Preto 14040-904, SP, BrazilDepartment of Basic and Oral Biology, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto 14040-904, SP, BrazilDepartment of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Av do Café, s/n., Ribeirao Preto 14040-904, SP, BrazilDepartment of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Av do Café, s/n., Ribeirao Preto 14040-904, SP, BrazilDepartment of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Av do Café, s/n., Ribeirao Preto 14040-904, SP, BrazilDepartment of Basic and Oral Biology, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto 14040-904, SP, BrazilDepartment of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Av do Café, s/n., Ribeirao Preto 14040-904, SP, BrazilDepartment of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Av do Café, s/n., Ribeirao Preto 14040-904, SP, BrazilThe aim of this study was to evaluate the use of a new porcine bone graft in rat calvaria bone defects. Critical defects were surgically created in 24 rats that were divided into four experimental groups according to defect filling (<i>n</i> = 6): Control Group (CG)—blood clot; Porcine Bone Group (PG)—porcine-derived bone substitute; (BG): Bio-Oss Group (BG)–chemically and heat-treated bovine graft; Bonefill Group (BFG)—chemically treated bovine bone substitute. Euthanasia of the animals occurred 30 days after the surgery, and the area of the original surgical defect and the surrounding tissues were removed for micro-CT and histomorphometric analysis. In the micro-CT evaluation, the PG presented statistically significant differences (<i>p</i> < 0.05) in comparison to the CG, BG and BFG, for the parameters percentage of Bone Volume (BV/TV), Surface Bone Density (BS/TV), Number of Trabeculae (Tb.N) and Bone Connectivity (Conn), but not for Total Porosity (Po.tot) and Trabecular Thickness (Tb.Th). The histomorphometric analysis showed that the PG presented similar results to the BG regarding newly formed bone extension and to the BG and BFG regarding newly formed bone area. The porcine-derived graft presented superior microtomographic and histomorphometric results when compared to the two bovine bone substitutes.https://www.mdpi.com/2079-4983/13/3/124bone regenerationbone substitutehistology |
| spellingShingle | Ytalo Fernandes Rafael Mantovani Danilo Reino Arthur Novaes Michel Messora Luiz Gustavo Sousa Daniela Palioto Sergio Scombatti de Souza Evaluation of a New Porcine Bone Graft on the Repair of Surgically Created Critical Bone Defects in Rat Calvaria: Histomorphometric and Microtomographic Study Journal of Functional Biomaterials bone regeneration bone substitute histology |
| title | Evaluation of a New Porcine Bone Graft on the Repair of Surgically Created Critical Bone Defects in Rat Calvaria: Histomorphometric and Microtomographic Study |
| title_full | Evaluation of a New Porcine Bone Graft on the Repair of Surgically Created Critical Bone Defects in Rat Calvaria: Histomorphometric and Microtomographic Study |
| title_fullStr | Evaluation of a New Porcine Bone Graft on the Repair of Surgically Created Critical Bone Defects in Rat Calvaria: Histomorphometric and Microtomographic Study |
| title_full_unstemmed | Evaluation of a New Porcine Bone Graft on the Repair of Surgically Created Critical Bone Defects in Rat Calvaria: Histomorphometric and Microtomographic Study |
| title_short | Evaluation of a New Porcine Bone Graft on the Repair of Surgically Created Critical Bone Defects in Rat Calvaria: Histomorphometric and Microtomographic Study |
| title_sort | evaluation of a new porcine bone graft on the repair of surgically created critical bone defects in rat calvaria histomorphometric and microtomographic study |
| topic | bone regeneration bone substitute histology |
| url | https://www.mdpi.com/2079-4983/13/3/124 |
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