Type III Secretion–Dependent and –Independent Phenotypes Caused by Ralstonia solanacearum in Arabidopsis Roots
The causal agent of bacterial wilt, Ralstonia solanacearum, is a soilborne pathogen that invades plants through their roots, traversing many tissue layers until it reaches the xylem, where it multiplies and causes plant collapse. The effects of R. solanacearum infection are devastating, and no effec...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2018-01-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-05-17-0109-FI |
| _version_ | 1818757356643680256 |
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| author | Haibin Lu Saul Lema A Marc Planas-Marquès Alejandro Alonso-Díaz Marc Valls Núria S. Coll |
| author_facet | Haibin Lu Saul Lema A Marc Planas-Marquès Alejandro Alonso-Díaz Marc Valls Núria S. Coll |
| author_sort | Haibin Lu |
| collection | DOAJ |
| description | The causal agent of bacterial wilt, Ralstonia solanacearum, is a soilborne pathogen that invades plants through their roots, traversing many tissue layers until it reaches the xylem, where it multiplies and causes plant collapse. The effects of R. solanacearum infection are devastating, and no effective approach to fight the disease is so far available. The early steps of infection, essential for colonization, as well as the early plant defense responses remain mostly unknown. Here, we have set up a simple, in vitro Arabidopsis thaliana–R. solanacearum pathosystem that has allowed us to identify three clear root phenotypes specifically associated to the early stages of infection: root-growth inhibition, root-hair formation, and root-tip cell death. Using this method, we have been able to differentiate, on Arabidopsis plants, the phenotypes caused by mutants in the key bacterial virulence regulators hrpB and hrpG, which remained indistinguishable using the classical soil-drench inoculation pathogenicity assays. In addition, we have revealed the previously unknown involvement of auxins in the root rearrangements caused by R. solanacearum infection. Our system provides an easy-to-use, high-throughput tool to study R. solanacearum aggressiveness. Furthermore, the observed phenotypes may allow the identification of bacterial virulence determinants and could even be used to screen for novel forms of early plant resistance to bacterial wilt. |
| first_indexed | 2024-12-18T06:09:38Z |
| format | Article |
| id | doaj.art-a52abec0258a4d1dbb45a56a56264536 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-18T06:09:38Z |
| publishDate | 2018-01-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-a52abec0258a4d1dbb45a56a562645362022-12-21T21:18:27ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062018-01-0131117518410.1094/MPMI-05-17-0109-FIType III Secretion–Dependent and –Independent Phenotypes Caused by Ralstonia solanacearum in Arabidopsis RootsHaibin Lu0Saul Lema A1Marc Planas-Marquès2Alejandro Alonso-Díaz3Marc Valls4Núria S. Coll5Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain; andCentre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain; andCentre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain; andCentre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain; andCentre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain; andCentre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain; andThe causal agent of bacterial wilt, Ralstonia solanacearum, is a soilborne pathogen that invades plants through their roots, traversing many tissue layers until it reaches the xylem, where it multiplies and causes plant collapse. The effects of R. solanacearum infection are devastating, and no effective approach to fight the disease is so far available. The early steps of infection, essential for colonization, as well as the early plant defense responses remain mostly unknown. Here, we have set up a simple, in vitro Arabidopsis thaliana–R. solanacearum pathosystem that has allowed us to identify three clear root phenotypes specifically associated to the early stages of infection: root-growth inhibition, root-hair formation, and root-tip cell death. Using this method, we have been able to differentiate, on Arabidopsis plants, the phenotypes caused by mutants in the key bacterial virulence regulators hrpB and hrpG, which remained indistinguishable using the classical soil-drench inoculation pathogenicity assays. In addition, we have revealed the previously unknown involvement of auxins in the root rearrangements caused by R. solanacearum infection. Our system provides an easy-to-use, high-throughput tool to study R. solanacearum aggressiveness. Furthermore, the observed phenotypes may allow the identification of bacterial virulence determinants and could even be used to screen for novel forms of early plant resistance to bacterial wilt.https://apsjournals.apsnet.org/doi/10.1094/MPMI-05-17-0109-FI |
| spellingShingle | Haibin Lu Saul Lema A Marc Planas-Marquès Alejandro Alonso-Díaz Marc Valls Núria S. Coll Type III Secretion–Dependent and –Independent Phenotypes Caused by Ralstonia solanacearum in Arabidopsis Roots Molecular Plant-Microbe Interactions |
| title | Type III Secretion–Dependent and –Independent Phenotypes Caused by Ralstonia solanacearum in Arabidopsis Roots |
| title_full | Type III Secretion–Dependent and –Independent Phenotypes Caused by Ralstonia solanacearum in Arabidopsis Roots |
| title_fullStr | Type III Secretion–Dependent and –Independent Phenotypes Caused by Ralstonia solanacearum in Arabidopsis Roots |
| title_full_unstemmed | Type III Secretion–Dependent and –Independent Phenotypes Caused by Ralstonia solanacearum in Arabidopsis Roots |
| title_short | Type III Secretion–Dependent and –Independent Phenotypes Caused by Ralstonia solanacearum in Arabidopsis Roots |
| title_sort | type iii secretion dependent and independent phenotypes caused by ralstonia solanacearum in arabidopsis roots |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-05-17-0109-FI |
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