Effects of Feeding Probiotics on the Localization of Cells Containing Immunoreactive Interleukin-6 in the Intestine of Broiler Chicks
The aim of this study was to examine whether treatments of chicks with probiotics affect the population of cells containing interleukin-6 (IL-6) and IgA in the intestine of broiler chicks. The diets with probiotics, Streptococcus faecalis, Clostridium buthricum, Bacillus mesentericus, were fed fro...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2010-07-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/47/3/47_010020/_pdf/-char/en |
| _version_ | 1797816201548660736 |
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| author | Yukinori Yoshimura Miwa Oda Naoki Isobe |
| author_facet | Yukinori Yoshimura Miwa Oda Naoki Isobe |
| author_sort | Yukinori Yoshimura |
| collection | DOAJ |
| description | The aim of this study was to examine whether treatments of chicks with probiotics affect the population of cells containing interleukin-6 (IL-6) and IgA in the intestine of broiler chicks. The diets with probiotics, Streptococcus faecalis, Clostridium buthricum, Bacillus mesentericus, were fed from day 0 to 10 of age. The sections of duodenum, pouch and tonsil region of cecum and colon were immunostained for IL-6 and IgA. The IL-6 immunoreaction products were identified in the leukocytes locating in the subepithelial tissues under the mucosal and crypt epithelium and lamina propria, and occationally in the the mucosal and crypt epithelium, in all birds. The density of immunoreactive (ir) IL-6 cell population in the mucosa tended to be lowest in the duodenum and highest in the colon. The density did not differ between control and probiotics groups on day 5; however, that in the cecum pouch and colon was significantly greater in the probiotics group than control on day 10. The IgA immunoreaction products were identified in the mucosal and crypt epithelial cells in all segments of the intestine, and some IgA-positive cells were localized in the lymphoid tissue in the cecum tonsil, whereas differences in their localization were not observed between probiotics and control groups. In conclusion, we suggest that probiotics increases the population of irIL-6 cells in the cecum and colon mucosal tissues of chicks. Because IL-6 is multifunctional cytokine for immune system, the increased IL-6 secretion may affect the immune functions in the intestinal mucosal tissues. |
| first_indexed | 2024-03-13T08:34:11Z |
| format | Article |
| id | doaj.art-a52baa907d0b4ddca35ce8eb3a421c42 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:34:11Z |
| publishDate | 2010-07-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-a52baa907d0b4ddca35ce8eb3a421c422023-05-31T00:55:35ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862010-07-0147325025510.2141/jpsa.010020jpsaEffects of Feeding Probiotics on the Localization of Cells Containing Immunoreactive Interleukin-6 in the Intestine of Broiler ChicksYukinori Yoshimura0Miwa Oda1Naoki Isobe2Graduate School of Biosphere Science, Hiroshima University, JapanGraduate School of Biosphere Science, Hiroshima University, JapanGraduate School of Biosphere Science, Hiroshima University, JapanThe aim of this study was to examine whether treatments of chicks with probiotics affect the population of cells containing interleukin-6 (IL-6) and IgA in the intestine of broiler chicks. The diets with probiotics, Streptococcus faecalis, Clostridium buthricum, Bacillus mesentericus, were fed from day 0 to 10 of age. The sections of duodenum, pouch and tonsil region of cecum and colon were immunostained for IL-6 and IgA. The IL-6 immunoreaction products were identified in the leukocytes locating in the subepithelial tissues under the mucosal and crypt epithelium and lamina propria, and occationally in the the mucosal and crypt epithelium, in all birds. The density of immunoreactive (ir) IL-6 cell population in the mucosa tended to be lowest in the duodenum and highest in the colon. The density did not differ between control and probiotics groups on day 5; however, that in the cecum pouch and colon was significantly greater in the probiotics group than control on day 10. The IgA immunoreaction products were identified in the mucosal and crypt epithelial cells in all segments of the intestine, and some IgA-positive cells were localized in the lymphoid tissue in the cecum tonsil, whereas differences in their localization were not observed between probiotics and control groups. In conclusion, we suggest that probiotics increases the population of irIL-6 cells in the cecum and colon mucosal tissues of chicks. Because IL-6 is multifunctional cytokine for immune system, the increased IL-6 secretion may affect the immune functions in the intestinal mucosal tissues.https://www.jstage.jst.go.jp/article/jpsa/47/3/47_010020/_pdf/-char/enchicksimmunoglobulin ainterleukin-6intestineprobiotics |
| spellingShingle | Yukinori Yoshimura Miwa Oda Naoki Isobe Effects of Feeding Probiotics on the Localization of Cells Containing Immunoreactive Interleukin-6 in the Intestine of Broiler Chicks The Journal of Poultry Science chicks immunoglobulin a interleukin-6 intestine probiotics |
| title | Effects of Feeding Probiotics on the Localization of Cells Containing Immunoreactive Interleukin-6 in the Intestine of Broiler Chicks |
| title_full | Effects of Feeding Probiotics on the Localization of Cells Containing Immunoreactive Interleukin-6 in the Intestine of Broiler Chicks |
| title_fullStr | Effects of Feeding Probiotics on the Localization of Cells Containing Immunoreactive Interleukin-6 in the Intestine of Broiler Chicks |
| title_full_unstemmed | Effects of Feeding Probiotics on the Localization of Cells Containing Immunoreactive Interleukin-6 in the Intestine of Broiler Chicks |
| title_short | Effects of Feeding Probiotics on the Localization of Cells Containing Immunoreactive Interleukin-6 in the Intestine of Broiler Chicks |
| title_sort | effects of feeding probiotics on the localization of cells containing immunoreactive interleukin 6 in the intestine of broiler chicks |
| topic | chicks immunoglobulin a interleukin-6 intestine probiotics |
| url | https://www.jstage.jst.go.jp/article/jpsa/47/3/47_010020/_pdf/-char/en |
| work_keys_str_mv | AT yukinoriyoshimura effectsoffeedingprobioticsonthelocalizationofcellscontainingimmunoreactiveinterleukin6intheintestineofbroilerchicks AT miwaoda effectsoffeedingprobioticsonthelocalizationofcellscontainingimmunoreactiveinterleukin6intheintestineofbroilerchicks AT naokiisobe effectsoffeedingprobioticsonthelocalizationofcellscontainingimmunoreactiveinterleukin6intheintestineofbroilerchicks |