Microbial Evaluation of Ozone Water Combined with Ultrasound Cleaning on Crayfish (<i>Procambarus clarkii</i>)
The effects of ozone water (OW) and ultrasound cleaning (UL) on microbial community diversity of crayfish were studied through microbial viable count and 16S rRNA gene sequencing. The results showed that compared with the control (CK), the ozone water combined with ultrasound cleaning (OCU) showed a...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/15/2314 |
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| author | Yuzhao Ling Hongyuan Tan Lingwei Shen Lingyun Wei Guangquan Xiong Lan Wang Wenjin Wu Yu Qiao |
| author_facet | Yuzhao Ling Hongyuan Tan Lingwei Shen Lingyun Wei Guangquan Xiong Lan Wang Wenjin Wu Yu Qiao |
| author_sort | Yuzhao Ling |
| collection | DOAJ |
| description | The effects of ozone water (OW) and ultrasound cleaning (UL) on microbial community diversity of crayfish were studied through microbial viable count and 16S rRNA gene sequencing. The results showed that compared with the control (CK), the ozone water combined with ultrasound cleaning (OCU) showed a significant reduction (<i>p</i> < 0.05) in total viable count (TVC), psychrophilic viable count (PVC), mesophilic viable count (MVC), Pseudomonas, hydrogen sulfide-producing bacteria (HSPB), molds and yeasts. Concretely, the TVC of the CK, OW, UL and OCU were 5.09, 4.55, 4.32 and 4.06 log CFU/g, respectively. The dominant bacterium in untreated crayfish was <i>Chryseobacterium</i>, and its relative abundance was reduced by combined treatment. Color measurement and sensory evaluation suggested that a satisfactory sensory experience could be obtained on the crayfish applied with OCU. In brief, OCU could be used as a cleaning strategy to control the microbial quality of crayfish and have no influence on its quality. |
| first_indexed | 2024-03-09T12:37:09Z |
| format | Article |
| id | doaj.art-a52cc20ec52b4abcae011d9d3a12ff8e |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T12:37:09Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-a52cc20ec52b4abcae011d9d3a12ff8e2023-11-30T22:22:55ZengMDPI AGFoods2304-81582022-08-011115231410.3390/foods11152314Microbial Evaluation of Ozone Water Combined with Ultrasound Cleaning on Crayfish (<i>Procambarus clarkii</i>)Yuzhao Ling0Hongyuan Tan1Lingwei Shen2Lingyun Wei3Guangquan Xiong4Lan Wang5Wenjin Wu6Yu Qiao7Key Laboratory of Cold Chain Logistics Technology for Agro-Product, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Cold Chain Logistics Technology for Agro-Product, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Cold Chain Logistics Technology for Agro-Product, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaSchool of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan 430205, ChinaKey Laboratory of Cold Chain Logistics Technology for Agro-Product, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Cold Chain Logistics Technology for Agro-Product, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Cold Chain Logistics Technology for Agro-Product, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Cold Chain Logistics Technology for Agro-Product, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaThe effects of ozone water (OW) and ultrasound cleaning (UL) on microbial community diversity of crayfish were studied through microbial viable count and 16S rRNA gene sequencing. The results showed that compared with the control (CK), the ozone water combined with ultrasound cleaning (OCU) showed a significant reduction (<i>p</i> < 0.05) in total viable count (TVC), psychrophilic viable count (PVC), mesophilic viable count (MVC), Pseudomonas, hydrogen sulfide-producing bacteria (HSPB), molds and yeasts. Concretely, the TVC of the CK, OW, UL and OCU were 5.09, 4.55, 4.32 and 4.06 log CFU/g, respectively. The dominant bacterium in untreated crayfish was <i>Chryseobacterium</i>, and its relative abundance was reduced by combined treatment. Color measurement and sensory evaluation suggested that a satisfactory sensory experience could be obtained on the crayfish applied with OCU. In brief, OCU could be used as a cleaning strategy to control the microbial quality of crayfish and have no influence on its quality.https://www.mdpi.com/2304-8158/11/15/2314crayfishozone waterultrasound16S rRNA gene sequencing |
| spellingShingle | Yuzhao Ling Hongyuan Tan Lingwei Shen Lingyun Wei Guangquan Xiong Lan Wang Wenjin Wu Yu Qiao Microbial Evaluation of Ozone Water Combined with Ultrasound Cleaning on Crayfish (<i>Procambarus clarkii</i>) Foods crayfish ozone water ultrasound 16S rRNA gene sequencing |
| title | Microbial Evaluation of Ozone Water Combined with Ultrasound Cleaning on Crayfish (<i>Procambarus clarkii</i>) |
| title_full | Microbial Evaluation of Ozone Water Combined with Ultrasound Cleaning on Crayfish (<i>Procambarus clarkii</i>) |
| title_fullStr | Microbial Evaluation of Ozone Water Combined with Ultrasound Cleaning on Crayfish (<i>Procambarus clarkii</i>) |
| title_full_unstemmed | Microbial Evaluation of Ozone Water Combined with Ultrasound Cleaning on Crayfish (<i>Procambarus clarkii</i>) |
| title_short | Microbial Evaluation of Ozone Water Combined with Ultrasound Cleaning on Crayfish (<i>Procambarus clarkii</i>) |
| title_sort | microbial evaluation of ozone water combined with ultrasound cleaning on crayfish i procambarus clarkii i |
| topic | crayfish ozone water ultrasound 16S rRNA gene sequencing |
| url | https://www.mdpi.com/2304-8158/11/15/2314 |
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