Whole Blood as a Sample Matrix in Homogeneous Time-Resolved Assay—Förster Resonance Energy Transfer-Based Antibody Detection
The protein-L-utilizing Förster resonance energy transfer (LFRET) assay enables mix-and-read antibody detection, as demonstrated for sera from patients with, e.g., severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Zika virus, and orthohantavirus infections. In this study, we compared pai...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/14/7/720 |
| _version_ | 1797212795752677376 |
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| author | Annika Lintala Olli Vapalahti Arttu Nousiainen Anu Kantele Jussi Hepojoki |
| author_facet | Annika Lintala Olli Vapalahti Arttu Nousiainen Anu Kantele Jussi Hepojoki |
| author_sort | Annika Lintala |
| collection | DOAJ |
| description | The protein-L-utilizing Förster resonance energy transfer (LFRET) assay enables mix-and-read antibody detection, as demonstrated for sera from patients with, e.g., severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Zika virus, and orthohantavirus infections. In this study, we compared paired serum and whole blood (WB) samples of COVID-19 patients and SARS-CoV-2 vaccine recipients. We found that LFRET also detects specific antibodies in WB samples. In 44 serum–WB pairs from patients with laboratory-confirmed COVID-19, LFRET showed a strong correlation between the sample materials. By analyzing 89 additional WB samples, totaling 133 WB samples, we found that LFRET results were moderately correlated with enzyme-linked immunosorbent assay results for samples collected 2 to 14 months after receiving COVID-19 diagnosis. However, the correlation decreased for samples >14 months after receiving a diagnosis. When comparing the WB LFRET results to neutralizing antibody titers, a strong correlation emerged for samples collected 1 to 14 months after receiving a diagnosis. This study also highlights the versatility of LFRET in detecting antibodies directly from WB samples and suggests that it could be employed for rapidly assessing antibody responses to infectious agents or vaccines. |
| first_indexed | 2024-04-24T10:48:04Z |
| format | Article |
| id | doaj.art-a532c182bb6d4f30b48d543c0f68461f |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-04-24T10:48:04Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-a532c182bb6d4f30b48d543c0f68461f2024-04-12T13:16:47ZengMDPI AGDiagnostics2075-44182024-03-0114772010.3390/diagnostics14070720Whole Blood as a Sample Matrix in Homogeneous Time-Resolved Assay—Förster Resonance Energy Transfer-Based Antibody DetectionAnnika Lintala0Olli Vapalahti1Arttu Nousiainen2Anu Kantele3Jussi Hepojoki4Department of Virology, Faculty of Medicine, Medicum, University of Helsinki, 00290 Helsinki, FinlandDepartment of Virology, Faculty of Medicine, Medicum, University of Helsinki, 00290 Helsinki, FinlandHuman Microbiome Research Program, Faculty of Medicine, University of Helsinki, 00014 Helsinki, FinlandHuman Microbiome Research Program, Faculty of Medicine, University of Helsinki, 00014 Helsinki, FinlandDepartment of Virology, Faculty of Medicine, Medicum, University of Helsinki, 00290 Helsinki, FinlandThe protein-L-utilizing Förster resonance energy transfer (LFRET) assay enables mix-and-read antibody detection, as demonstrated for sera from patients with, e.g., severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Zika virus, and orthohantavirus infections. In this study, we compared paired serum and whole blood (WB) samples of COVID-19 patients and SARS-CoV-2 vaccine recipients. We found that LFRET also detects specific antibodies in WB samples. In 44 serum–WB pairs from patients with laboratory-confirmed COVID-19, LFRET showed a strong correlation between the sample materials. By analyzing 89 additional WB samples, totaling 133 WB samples, we found that LFRET results were moderately correlated with enzyme-linked immunosorbent assay results for samples collected 2 to 14 months after receiving COVID-19 diagnosis. However, the correlation decreased for samples >14 months after receiving a diagnosis. When comparing the WB LFRET results to neutralizing antibody titers, a strong correlation emerged for samples collected 1 to 14 months after receiving a diagnosis. This study also highlights the versatility of LFRET in detecting antibodies directly from WB samples and suggests that it could be employed for rapidly assessing antibody responses to infectious agents or vaccines.https://www.mdpi.com/2075-4418/14/7/720LFRETwhole bloodSARS-CoV-2COVID-19antibody detection |
| spellingShingle | Annika Lintala Olli Vapalahti Arttu Nousiainen Anu Kantele Jussi Hepojoki Whole Blood as a Sample Matrix in Homogeneous Time-Resolved Assay—Förster Resonance Energy Transfer-Based Antibody Detection Diagnostics LFRET whole blood SARS-CoV-2 COVID-19 antibody detection |
| title | Whole Blood as a Sample Matrix in Homogeneous Time-Resolved Assay—Förster Resonance Energy Transfer-Based Antibody Detection |
| title_full | Whole Blood as a Sample Matrix in Homogeneous Time-Resolved Assay—Förster Resonance Energy Transfer-Based Antibody Detection |
| title_fullStr | Whole Blood as a Sample Matrix in Homogeneous Time-Resolved Assay—Förster Resonance Energy Transfer-Based Antibody Detection |
| title_full_unstemmed | Whole Blood as a Sample Matrix in Homogeneous Time-Resolved Assay—Förster Resonance Energy Transfer-Based Antibody Detection |
| title_short | Whole Blood as a Sample Matrix in Homogeneous Time-Resolved Assay—Förster Resonance Energy Transfer-Based Antibody Detection |
| title_sort | whole blood as a sample matrix in homogeneous time resolved assay forster resonance energy transfer based antibody detection |
| topic | LFRET whole blood SARS-CoV-2 COVID-19 antibody detection |
| url | https://www.mdpi.com/2075-4418/14/7/720 |
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