Inhibitory Effects of <i>Aucklandia lappa</i> Decne. Extract on Inflammatory and Oxidative Responses in LPS-Treated Macrophages
<i>Aucklandia lappa</i> Decne., known as “Mok-hyang” in Korea, has been used for the alleviation of abdominal pain, vomiting, diarrhea, and stress gastric ulcers in traditional oriental medicine. We investigated the anti-inflammatory and antioxidative effects of the e...
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MDPI AG
2020-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/6/1336 |
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| author | Jae Sung Lim Sung Ho Lee Sang Rok Lee Hyung-Ju Lim Yoon-Seok Roh Eun Jeong Won Namki Cho Changju Chun Young-Chang Cho |
| author_facet | Jae Sung Lim Sung Ho Lee Sang Rok Lee Hyung-Ju Lim Yoon-Seok Roh Eun Jeong Won Namki Cho Changju Chun Young-Chang Cho |
| author_sort | Jae Sung Lim |
| collection | DOAJ |
| description | <i>Aucklandia lappa</i> Decne., known as “Mok-hyang” in Korea, has been used for the alleviation of abdominal pain, vomiting, diarrhea, and stress gastric ulcers in traditional oriental medicine. We investigated the anti-inflammatory and antioxidative effects of the ethanol extract of <i>Aucklandia lappa</i> Decne. (ALDE) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. ALDE significantly inhibited the LPS-induced nitric oxide (NO) production and reduced inducible nitric oxide synthase (iNOS) expression in RAW 264.7 cells. The production of other proinflammatory mediators, including COX-2, interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF)-α, was reduced by ALDE in LPS-stimulated RAW 264.7 cells. The mechanism underlying the anti-inflammatory effects of ALDE was elucidated to be the suppression of LPS-induced nuclear translocation of p65, followed by the degradation of IκB and the inhibition of the phosphorylation of mitogen-activated protein kinases (MAPK). In addition, ALDE showed enhanced radical scavenging activity. The antioxidant effect of ALDE was caused by the enhanced expression of heme oxygenase (HO-1) via stabilization of the expression of the nuclear transcription factor E2-related factor 2 (Nrf2) pathway. Collectively, these results indicated that ALDE not only exerts anti-inflammatory effects via the suppression of the NF-κB and MAPK pathways but also has an antioxidative effect through the activation of the Nrf2/HO-1 pathway. |
| first_indexed | 2024-12-23T23:45:13Z |
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| language | English |
| last_indexed | 2024-12-23T23:45:13Z |
| publishDate | 2020-03-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a534b8f07743493a92ff28ece0e7b3b22022-12-21T17:25:32ZengMDPI AGMolecules1420-30492020-03-01256133610.3390/molecules25061336molecules25061336Inhibitory Effects of <i>Aucklandia lappa</i> Decne. Extract on Inflammatory and Oxidative Responses in LPS-Treated MacrophagesJae Sung Lim0Sung Ho Lee1Sang Rok Lee2Hyung-Ju Lim3Yoon-Seok Roh4Eun Jeong Won5Namki Cho6Changju Chun7Young-Chang Cho8Department of Biochemistry, Chonnam National University Medical School, Hwasun, Jeonnam-do 58128, KoreaDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USAROK-Biotech, Jeollanamdo Biopharmaceutical Research Center, Hwasun, Jeollanam-do 58141, KoreaCombinatorial Tumor Immunotherapy Medical Research Center, Chonnam National University Medical School, Hwasun, Jeonnam-do 58128, KoreaCollege of Pharmacy and Medical Research Center, Chungbuk National University, Cheongju 28160, KoreaDepartment of Parasitology and Tropical Medicine, Chonnam National University Medical School, Hwasun, Jeonnam-do 58128, KoreaCollege of Pharmacy, Chonnam National University, Gwangju 61186, KoreaCollege of Pharmacy, Chonnam National University, Gwangju 61186, KoreaCollege of Pharmacy, Chonnam National University, Gwangju 61186, Korea<i>Aucklandia lappa</i> Decne., known as “Mok-hyang” in Korea, has been used for the alleviation of abdominal pain, vomiting, diarrhea, and stress gastric ulcers in traditional oriental medicine. We investigated the anti-inflammatory and antioxidative effects of the ethanol extract of <i>Aucklandia lappa</i> Decne. (ALDE) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. ALDE significantly inhibited the LPS-induced nitric oxide (NO) production and reduced inducible nitric oxide synthase (iNOS) expression in RAW 264.7 cells. The production of other proinflammatory mediators, including COX-2, interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF)-α, was reduced by ALDE in LPS-stimulated RAW 264.7 cells. The mechanism underlying the anti-inflammatory effects of ALDE was elucidated to be the suppression of LPS-induced nuclear translocation of p65, followed by the degradation of IκB and the inhibition of the phosphorylation of mitogen-activated protein kinases (MAPK). In addition, ALDE showed enhanced radical scavenging activity. The antioxidant effect of ALDE was caused by the enhanced expression of heme oxygenase (HO-1) via stabilization of the expression of the nuclear transcription factor E2-related factor 2 (Nrf2) pathway. Collectively, these results indicated that ALDE not only exerts anti-inflammatory effects via the suppression of the NF-κB and MAPK pathways but also has an antioxidative effect through the activation of the Nrf2/HO-1 pathway.https://www.mdpi.com/1420-3049/25/6/1336<i>aucklandia lappa</i> decne. extractlipopolysaccharidenitric oxideinducible nitric oxidecyclooxygenase-2nuclear factor-κbmitogen-activated protein kinaseheme oxygenase-1macrophage |
| spellingShingle | Jae Sung Lim Sung Ho Lee Sang Rok Lee Hyung-Ju Lim Yoon-Seok Roh Eun Jeong Won Namki Cho Changju Chun Young-Chang Cho Inhibitory Effects of <i>Aucklandia lappa</i> Decne. Extract on Inflammatory and Oxidative Responses in LPS-Treated Macrophages Molecules <i>aucklandia lappa</i> decne. extract lipopolysaccharide nitric oxide inducible nitric oxide cyclooxygenase-2 nuclear factor-κb mitogen-activated protein kinase heme oxygenase-1 macrophage |
| title | Inhibitory Effects of <i>Aucklandia lappa</i> Decne. Extract on Inflammatory and Oxidative Responses in LPS-Treated Macrophages |
| title_full | Inhibitory Effects of <i>Aucklandia lappa</i> Decne. Extract on Inflammatory and Oxidative Responses in LPS-Treated Macrophages |
| title_fullStr | Inhibitory Effects of <i>Aucklandia lappa</i> Decne. Extract on Inflammatory and Oxidative Responses in LPS-Treated Macrophages |
| title_full_unstemmed | Inhibitory Effects of <i>Aucklandia lappa</i> Decne. Extract on Inflammatory and Oxidative Responses in LPS-Treated Macrophages |
| title_short | Inhibitory Effects of <i>Aucklandia lappa</i> Decne. Extract on Inflammatory and Oxidative Responses in LPS-Treated Macrophages |
| title_sort | inhibitory effects of i aucklandia lappa i decne extract on inflammatory and oxidative responses in lps treated macrophages |
| topic | <i>aucklandia lappa</i> decne. extract lipopolysaccharide nitric oxide inducible nitric oxide cyclooxygenase-2 nuclear factor-κb mitogen-activated protein kinase heme oxygenase-1 macrophage |
| url | https://www.mdpi.com/1420-3049/25/6/1336 |
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