Effects of Oligosaccharides Isolated From Pinewood Hot Water Pre-hydrolyzates on Recombinant Cellulases
Loblolly pine residues have enormous potential to be the raw material for advanced biofuel production due to extensive sources and high cellulose content. Hot water (HW) pretreatment, while being a relatively economical and clean technology for the deconstruction of lignocellulosic biomass, could al...
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| Format: | Article |
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Frontiers Media S.A.
2018-05-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fbioe.2018.00055/full |
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| author | Hong Fang Gurshagan Kandhola Kalavathy Rajan Angele Djioleu Danielle Julie Carrier Kendall R. Hood Elizabeth E. Hood |
| author_facet | Hong Fang Gurshagan Kandhola Kalavathy Rajan Angele Djioleu Danielle Julie Carrier Kendall R. Hood Elizabeth E. Hood |
| author_sort | Hong Fang |
| collection | DOAJ |
| description | Loblolly pine residues have enormous potential to be the raw material for advanced biofuel production due to extensive sources and high cellulose content. Hot water (HW) pretreatment, while being a relatively economical and clean technology for the deconstruction of lignocellulosic biomass, could also inhibit the ensuing enzymatic hydrolysis process because of the production of inhibitors. In this study, we investigated the effect of oligosaccharide fractions purified from HW pre-hydrolyzate of pinewood using centrifugal partition chromatography (CPC) on three recombinant cellulolytic enzymes (E1, CBHI and CBHII), which were expressed in the transgenic corn grain system. The efficiency of recombinant enzymes was measured using either a 4-methylumbelliferyl-β-D-cellobioside (MUC) or a cellulose-dinitrosalicylic acid (DNS) assay system. The results showed that HW pre-hydrolyzate CPC fractions contain phenolics, furans, and monomeric and oligomeric sugars. Among CPC fractions, oligomers composed of xylan, galactan, and mannan were inhibitory to the three recombinant enzymes and to the commercial cellulase cocktail, reducing the enzymatic efficiency to as low as 10%. |
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| id | doaj.art-a535cb2e5d1943f2a0f2e10cc7c89411 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-12T18:25:29Z |
| publishDate | 2018-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-a535cb2e5d1943f2a0f2e10cc7c894112022-12-22T00:16:02ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852018-05-01610.3389/fbioe.2018.00055304064Effects of Oligosaccharides Isolated From Pinewood Hot Water Pre-hydrolyzates on Recombinant CellulasesHong Fang0Gurshagan Kandhola1Kalavathy Rajan2Angele Djioleu3Danielle Julie Carrier4Kendall R. Hood5Elizabeth E. Hood6Department of Biology, Molecular Biosciences Graduate Program, College of Biology, Molecular Biosciences, Arkansas State University, Jonesboro, AR, United StatesDepartment of Biological & Agricultural Engineering, University of Arkansas, Fayetteville, AR, United StatesDepartment of Biosystems Engineering and Soil Science, University of Tennessee, Knoxville, TN, United StatesDepartment of Biological & Agricultural Engineering, University of Arkansas, Fayetteville, AR, United StatesDepartment of Biosystems Engineering and Soil Science, University of Tennessee, Knoxville, TN, United StatesInfinite Enzymes, Arkansas State University, Jonesboro, AR, United StatesArkansas State University Biosciences Institute and College of Agriculture and Technology, Arkansas State University, Jonesboro, AR, United StatesLoblolly pine residues have enormous potential to be the raw material for advanced biofuel production due to extensive sources and high cellulose content. Hot water (HW) pretreatment, while being a relatively economical and clean technology for the deconstruction of lignocellulosic biomass, could also inhibit the ensuing enzymatic hydrolysis process because of the production of inhibitors. In this study, we investigated the effect of oligosaccharide fractions purified from HW pre-hydrolyzate of pinewood using centrifugal partition chromatography (CPC) on three recombinant cellulolytic enzymes (E1, CBHI and CBHII), which were expressed in the transgenic corn grain system. The efficiency of recombinant enzymes was measured using either a 4-methylumbelliferyl-β-D-cellobioside (MUC) or a cellulose-dinitrosalicylic acid (DNS) assay system. The results showed that HW pre-hydrolyzate CPC fractions contain phenolics, furans, and monomeric and oligomeric sugars. Among CPC fractions, oligomers composed of xylan, galactan, and mannan were inhibitory to the three recombinant enzymes and to the commercial cellulase cocktail, reducing the enzymatic efficiency to as low as 10%.http://journal.frontiersin.org/article/10.3389/fbioe.2018.00055/fullloblolly pinewoodhot water pretreatmentcentrifugal partition chromatographyrecombinant cellulasecellulolytic enzymes inhibitioncellulose digestion |
| spellingShingle | Hong Fang Gurshagan Kandhola Kalavathy Rajan Angele Djioleu Danielle Julie Carrier Kendall R. Hood Elizabeth E. Hood Effects of Oligosaccharides Isolated From Pinewood Hot Water Pre-hydrolyzates on Recombinant Cellulases Frontiers in Bioengineering and Biotechnology loblolly pinewood hot water pretreatment centrifugal partition chromatography recombinant cellulase cellulolytic enzymes inhibition cellulose digestion |
| title | Effects of Oligosaccharides Isolated From Pinewood Hot Water Pre-hydrolyzates on Recombinant Cellulases |
| title_full | Effects of Oligosaccharides Isolated From Pinewood Hot Water Pre-hydrolyzates on Recombinant Cellulases |
| title_fullStr | Effects of Oligosaccharides Isolated From Pinewood Hot Water Pre-hydrolyzates on Recombinant Cellulases |
| title_full_unstemmed | Effects of Oligosaccharides Isolated From Pinewood Hot Water Pre-hydrolyzates on Recombinant Cellulases |
| title_short | Effects of Oligosaccharides Isolated From Pinewood Hot Water Pre-hydrolyzates on Recombinant Cellulases |
| title_sort | effects of oligosaccharides isolated from pinewood hot water pre hydrolyzates on recombinant cellulases |
| topic | loblolly pinewood hot water pretreatment centrifugal partition chromatography recombinant cellulase cellulolytic enzymes inhibition cellulose digestion |
| url | http://journal.frontiersin.org/article/10.3389/fbioe.2018.00055/full |
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