The elucidation of plasma lipidome profiles during severe influenza in a mouse model
Abstract Although influenza virus infection has been shown to affect lipid metabolism, details remain unknown. Therefore, we elucidated the kinetic lipid profiles of mice infected with different doses of influenza virus A/Puerto Rico/8/34 (H1N1) (PR8) by measuring multiple lipid molecular species us...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-41055-y |
| _version_ | 1827634615669489664 |
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| author | Marumi Ohno Siddabasave Gowda B. Gowda Toshiki Sekiya Naoki Nomura Masashi Shingai Shu-Ping Hui Hiroshi Kida |
| author_facet | Marumi Ohno Siddabasave Gowda B. Gowda Toshiki Sekiya Naoki Nomura Masashi Shingai Shu-Ping Hui Hiroshi Kida |
| author_sort | Marumi Ohno |
| collection | DOAJ |
| description | Abstract Although influenza virus infection has been shown to affect lipid metabolism, details remain unknown. Therefore, we elucidated the kinetic lipid profiles of mice infected with different doses of influenza virus A/Puerto Rico/8/34 (H1N1) (PR8) by measuring multiple lipid molecular species using untargeted lipidomic analysis. C57BL/6 male mice were intranasally infected with PR8 virus at 50 or 500 plaque-forming units to cause sublethal or lethal influenza, respectively. Plasma and tissue samples were collected at 1, 3, and 6 days post-infection (dpi), and comprehensive lipidomic analysis was performed using high-performance liquid chromatography–linear trap quadrupole–Orbitrap mass spectrometry, as well as gene expression analyses. The most prominent feature of the lipid profile in lethally infected mice was the elevated plasma concentrations of phosphatidylethanolamines (PEs) containing polyunsaturated fatty acid (PUFA) at 3 dpi. Furthermore, the facilitation of PUFA-containing phospholipid production in the lungs, but not in the liver, was suggested by gene expression and lipidomic analysis of tissue samples. Given the increased plasma or serum levels of PUFA-containing PEs in patients with other viral infections, especially in severe cases, the elevation of these phospholipids in circulation could be a biomarker of infection and the severity of infectious diseases. |
| first_indexed | 2024-03-09T15:15:48Z |
| format | Article |
| id | doaj.art-a5368acb3dee4c4e8947405d262675ae |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:15:48Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a5368acb3dee4c4e8947405d262675ae2023-11-26T13:05:34ZengNature PortfolioScientific Reports2045-23222023-08-0113111210.1038/s41598-023-41055-yThe elucidation of plasma lipidome profiles during severe influenza in a mouse modelMarumi Ohno0Siddabasave Gowda B. Gowda1Toshiki Sekiya2Naoki Nomura3Masashi Shingai4Shu-Ping Hui5Hiroshi Kida6Division of Biologics Development, International Institute for Zoonosis Control, Hokkaido UniversityFaculty of Health Sciences, Hokkaido UniversityDivision of Biologics Development, International Institute for Zoonosis Control, Hokkaido UniversityDivision of Biologics Development, International Institute for Zoonosis Control, Hokkaido UniversityDivision of Biologics Development, International Institute for Zoonosis Control, Hokkaido UniversityFaculty of Health Sciences, Hokkaido UniversityDivision of Biologics Development, International Institute for Zoonosis Control, Hokkaido UniversityAbstract Although influenza virus infection has been shown to affect lipid metabolism, details remain unknown. Therefore, we elucidated the kinetic lipid profiles of mice infected with different doses of influenza virus A/Puerto Rico/8/34 (H1N1) (PR8) by measuring multiple lipid molecular species using untargeted lipidomic analysis. C57BL/6 male mice were intranasally infected with PR8 virus at 50 or 500 plaque-forming units to cause sublethal or lethal influenza, respectively. Plasma and tissue samples were collected at 1, 3, and 6 days post-infection (dpi), and comprehensive lipidomic analysis was performed using high-performance liquid chromatography–linear trap quadrupole–Orbitrap mass spectrometry, as well as gene expression analyses. The most prominent feature of the lipid profile in lethally infected mice was the elevated plasma concentrations of phosphatidylethanolamines (PEs) containing polyunsaturated fatty acid (PUFA) at 3 dpi. Furthermore, the facilitation of PUFA-containing phospholipid production in the lungs, but not in the liver, was suggested by gene expression and lipidomic analysis of tissue samples. Given the increased plasma or serum levels of PUFA-containing PEs in patients with other viral infections, especially in severe cases, the elevation of these phospholipids in circulation could be a biomarker of infection and the severity of infectious diseases.https://doi.org/10.1038/s41598-023-41055-y |
| spellingShingle | Marumi Ohno Siddabasave Gowda B. Gowda Toshiki Sekiya Naoki Nomura Masashi Shingai Shu-Ping Hui Hiroshi Kida The elucidation of plasma lipidome profiles during severe influenza in a mouse model Scientific Reports |
| title | The elucidation of plasma lipidome profiles during severe influenza in a mouse model |
| title_full | The elucidation of plasma lipidome profiles during severe influenza in a mouse model |
| title_fullStr | The elucidation of plasma lipidome profiles during severe influenza in a mouse model |
| title_full_unstemmed | The elucidation of plasma lipidome profiles during severe influenza in a mouse model |
| title_short | The elucidation of plasma lipidome profiles during severe influenza in a mouse model |
| title_sort | elucidation of plasma lipidome profiles during severe influenza in a mouse model |
| url | https://doi.org/10.1038/s41598-023-41055-y |
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