Phytophthora pluvialis maintenance, spore production and detached needle assays.
Phytophthora pluvialis is an oomycete that primarily infects Pinus radiata and Pseudotsuga menziesii causing the destructive foliar disease red needle cast (RNC). Recent observations show that P. pluvialis can also infect western hemlock inducing resinous cankers. High-throughput and reproducible in...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0293817 |
| _version_ | 1827307793376346112 |
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| author | Sophie Eccersall Leann S Vinson Rebecca McDougal Claudia-Nicole Meisrimler |
| author_facet | Sophie Eccersall Leann S Vinson Rebecca McDougal Claudia-Nicole Meisrimler |
| author_sort | Sophie Eccersall |
| collection | DOAJ |
| description | Phytophthora pluvialis is an oomycete that primarily infects Pinus radiata and Pseudotsuga menziesii causing the destructive foliar disease red needle cast (RNC). Recent observations show that P. pluvialis can also infect western hemlock inducing resinous cankers. High-throughput and reproducible infection assays are integral to find key information on tree health and oomycete pathogenicity. In this protocol, we describe the propagation and spore induction of P. pluvialis, followed by detached needle assays for verification and quantification of virulence of P. pluvialis in P. radiata needles. These needle assays can be employed for high-throughput screening of tree needles with diverse genetic backgrounds. In downstream analysis, Quantitative PCR (qPCR) was utilized to assess relative gene expression, as exemplified by candidate RxLR effector protein PpR01. Additional techniques like RNA sequencing, metabolomics, and proteomics can be combined with needle assays and can offer comprehensive insights into P. pluvialis infection mechanisms. |
| first_indexed | 2024-04-24T18:45:34Z |
| format | Article |
| id | doaj.art-a536c7cbe35449b79560af9e393a74f7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-24T18:45:34Z |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a536c7cbe35449b79560af9e393a74f72024-03-27T05:32:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01193e029381710.1371/journal.pone.0293817Phytophthora pluvialis maintenance, spore production and detached needle assays.Sophie EccersallLeann S VinsonRebecca McDougalClaudia-Nicole MeisrimlerPhytophthora pluvialis is an oomycete that primarily infects Pinus radiata and Pseudotsuga menziesii causing the destructive foliar disease red needle cast (RNC). Recent observations show that P. pluvialis can also infect western hemlock inducing resinous cankers. High-throughput and reproducible infection assays are integral to find key information on tree health and oomycete pathogenicity. In this protocol, we describe the propagation and spore induction of P. pluvialis, followed by detached needle assays for verification and quantification of virulence of P. pluvialis in P. radiata needles. These needle assays can be employed for high-throughput screening of tree needles with diverse genetic backgrounds. In downstream analysis, Quantitative PCR (qPCR) was utilized to assess relative gene expression, as exemplified by candidate RxLR effector protein PpR01. Additional techniques like RNA sequencing, metabolomics, and proteomics can be combined with needle assays and can offer comprehensive insights into P. pluvialis infection mechanisms.https://doi.org/10.1371/journal.pone.0293817 |
| spellingShingle | Sophie Eccersall Leann S Vinson Rebecca McDougal Claudia-Nicole Meisrimler Phytophthora pluvialis maintenance, spore production and detached needle assays. PLoS ONE |
| title | Phytophthora pluvialis maintenance, spore production and detached needle assays. |
| title_full | Phytophthora pluvialis maintenance, spore production and detached needle assays. |
| title_fullStr | Phytophthora pluvialis maintenance, spore production and detached needle assays. |
| title_full_unstemmed | Phytophthora pluvialis maintenance, spore production and detached needle assays. |
| title_short | Phytophthora pluvialis maintenance, spore production and detached needle assays. |
| title_sort | phytophthora pluvialis maintenance spore production and detached needle assays |
| url | https://doi.org/10.1371/journal.pone.0293817 |
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