| Summary: | In this study, <i>Salvia officinalis</i> L. and <i>Salvia sclarea</i> essential oils (EOs) were investigated using gas chromatography-mass spectrometry (GC-MS) to describe their chemical composition. The obtained results show, for both EOs, a profile rich in terpene metabolites, with monoterpenes predominating sesquiterpenes but with significant qualitative and quantitative differences. The main compound found in the <i>Salvia officinalis</i> EO (<i>SO</i>EO) was camphor (19.0%), while in <i>Salvia sclarea</i> EO (<i>SC</i>EO), it was linalyl acetate (59.3%). Subsequently, the in vitro antimicrobial activity of the EOs against eight pathogenic strains was evaluated. The disc diffusion method showed a significant lysis zone against Gram-positive bacteria. The minimum inhibitory concentrations (MICs) ranged from 3.7 mg/mL to 11.2 mg/mL, indicating that each EO has specific antimicrobial activity. Both EOs also showed significant antiradical activity against DPPH radicals and total antioxidant activity. In addition, the preservative effect of <i>SO</i>EO (9.2%) and <i>SC</i>EO (9.2%), alone or in combination, was tested in ground beef, and the inhibitory effect against <i>Listeria monocytogenes</i> inoculated into the raw ground beef during cold storage was evaluated. Although the effect of each individual EO improved the biochemical, microbiological, and sensory parameters of the samples, their combination was more effective and showed complete inhibition of <i>L. monocytogenes</i> after 7 days of storage at 4 °C. The results show that both EOs could be used as safe and natural preservatives in various food and/or pharmaceutical products.
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