Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic
New antibiotics are seen as ‘drugs of last resort’ against virulent bacteria. However, development of resistance towards new antibiotics with time is a universal fact. Delafloxacin (DFX) is a new fluoroquinolone antibiotic that differs from existing fluoroquinolones by the lack of a protonatable sub...
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MDPI AG
2022-08-01
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| Online Access: | https://www.mdpi.com/1996-1944/15/16/5709 |
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| author | Amr Selim Abu Lila Bader Huwaimel Ahmed Alobaida Talib Hussain Zeeshan Rafi Khalid Mehmood Marwa H. Abdallah Turki Al Hagbani Syed Mohd Danish Rizvi Afrasim Moin Abobakr F. Ahmed |
| author_facet | Amr Selim Abu Lila Bader Huwaimel Ahmed Alobaida Talib Hussain Zeeshan Rafi Khalid Mehmood Marwa H. Abdallah Turki Al Hagbani Syed Mohd Danish Rizvi Afrasim Moin Abobakr F. Ahmed |
| author_sort | Amr Selim Abu Lila |
| collection | DOAJ |
| description | New antibiotics are seen as ‘drugs of last resort’ against virulent bacteria. However, development of resistance towards new antibiotics with time is a universal fact. Delafloxacin (DFX) is a new fluoroquinolone antibiotic that differs from existing fluoroquinolones by the lack of a protonatable substituent, which gives the molecule a weakly acidic nature, affording it higher antibacterial activity under an acidic environment. Furthermore, antibiotic-functionalized metallic nanoparticles have been recently emerged as a feasible platform for conquering bacterial resistance. In the present study, therefore, we aimed at preparing DFX-gold nano-formulations to increase the antibacterial potential of DFX. To synthesize DFX-capped gold nanoparticles (DFX-AuNPs), DFX was used as a reducing and stabilizing/encapsulating agent. Various analytical techniques such as UV-visible spectroscopy, TEM, DLS, FTIR and zeta potential analysis were applied to determine the properties of the synthesized DFX-AuNPs. The synthesized DFX-AuNPs revealed a distinct surface plasmon resonance (SPR) band at 530 nm and an average size of 16 nm as manifested by TEM analysis. In addition, Zeta potential results (−19 mV) confirmed the stability of the synthesized DFX-AuNPs. Furthermore, FTIR analysis demonstrated that DFX was adsorbed onto the surface of AuNPs via strong interaction between AuNPs and DFX. Most importantly, comparative antibacterial analysis of DFX alone and DFX-AuNPs against Gram-negative (<i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i>) and Gram-positive (<i>Staphylococcus aureus</i> and <i>Bacillus subtilis</i>) verified the superior antibacterial activity of DFX-AuNPs against the tested microorganisms. To sum up, DFX gold nano-formulations can offer a promising possible solution, even at a lower antibiotic dose, to combat pathogenic bacteria. |
| first_indexed | 2024-03-09T04:09:53Z |
| format | Article |
| id | doaj.art-a5380f2e6e1645b1ac64fb73c83f32d7 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T04:09:53Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-a5380f2e6e1645b1ac64fb73c83f32d72023-12-03T14:02:17ZengMDPI AGMaterials1996-19442022-08-011516570910.3390/ma15165709Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone AntibioticAmr Selim Abu Lila0Bader Huwaimel1Ahmed Alobaida2Talib Hussain3Zeeshan Rafi4Khalid Mehmood5Marwa H. Abdallah6Turki Al Hagbani7Syed Mohd Danish Rizvi8Afrasim Moin9Abobakr F. Ahmed10Department of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaNanomedicine and Nanotechnology Lab, Department of Biosciences, Integral University, Lucknow 226026, IndiaDepartment of Pharmacy, Abbottabad University of Science and Technology, Havelian 22500, PakistanDepartment of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia 61519, EgyptNew antibiotics are seen as ‘drugs of last resort’ against virulent bacteria. However, development of resistance towards new antibiotics with time is a universal fact. Delafloxacin (DFX) is a new fluoroquinolone antibiotic that differs from existing fluoroquinolones by the lack of a protonatable substituent, which gives the molecule a weakly acidic nature, affording it higher antibacterial activity under an acidic environment. Furthermore, antibiotic-functionalized metallic nanoparticles have been recently emerged as a feasible platform for conquering bacterial resistance. In the present study, therefore, we aimed at preparing DFX-gold nano-formulations to increase the antibacterial potential of DFX. To synthesize DFX-capped gold nanoparticles (DFX-AuNPs), DFX was used as a reducing and stabilizing/encapsulating agent. Various analytical techniques such as UV-visible spectroscopy, TEM, DLS, FTIR and zeta potential analysis were applied to determine the properties of the synthesized DFX-AuNPs. The synthesized DFX-AuNPs revealed a distinct surface plasmon resonance (SPR) band at 530 nm and an average size of 16 nm as manifested by TEM analysis. In addition, Zeta potential results (−19 mV) confirmed the stability of the synthesized DFX-AuNPs. Furthermore, FTIR analysis demonstrated that DFX was adsorbed onto the surface of AuNPs via strong interaction between AuNPs and DFX. Most importantly, comparative antibacterial analysis of DFX alone and DFX-AuNPs against Gram-negative (<i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i>) and Gram-positive (<i>Staphylococcus aureus</i> and <i>Bacillus subtilis</i>) verified the superior antibacterial activity of DFX-AuNPs against the tested microorganisms. To sum up, DFX gold nano-formulations can offer a promising possible solution, even at a lower antibiotic dose, to combat pathogenic bacteria.https://www.mdpi.com/1996-1944/15/16/5709antibiotic resistancedelafloxacinfluoroquinolonegold nanoparticlesnano-formulations |
| spellingShingle | Amr Selim Abu Lila Bader Huwaimel Ahmed Alobaida Talib Hussain Zeeshan Rafi Khalid Mehmood Marwa H. Abdallah Turki Al Hagbani Syed Mohd Danish Rizvi Afrasim Moin Abobakr F. Ahmed Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic Materials antibiotic resistance delafloxacin fluoroquinolone gold nanoparticles nano-formulations |
| title | Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic |
| title_full | Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic |
| title_fullStr | Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic |
| title_full_unstemmed | Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic |
| title_short | Delafloxacin-Capped Gold Nanoparticles (DFX-AuNPs): An Effective Antibacterial Nano-Formulation of Fluoroquinolone Antibiotic |
| title_sort | delafloxacin capped gold nanoparticles dfx aunps an effective antibacterial nano formulation of fluoroquinolone antibiotic |
| topic | antibiotic resistance delafloxacin fluoroquinolone gold nanoparticles nano-formulations |
| url | https://www.mdpi.com/1996-1944/15/16/5709 |
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