Genomic insights into strategies used by <it>Xanthomonas albilineans</it> with its reduced artillery to spread within sugarcane xylem vessels
<p>Abstract</p> <p>Background</p> <p><it>Xanthomonas albilineans</it> causes leaf scald, a lethal disease of sugarcane. <it>X. albilineans</it> exhibits distinctive pathogenic mechanisms, ecology and taxonomy compared to other species of <it&g...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-11-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/13/658 |
| _version_ | 1811250742194864128 |
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| author | Pieretti Isabelle Royer Monique Barbe Valérie Carrere Sébastien Koebnik Ralf Couloux Arnaud Darrasse Armelle Gouzy Jérôme Jacques Marie-Agnès Lauber Emmanuelle Manceau Charles Mangenot Sophie Poussier Stéphane Segurens Béatrice Szurek Boris Verdier Valérie Arlat Matthieu Gabriel Dean W Rott Philippe Cociancich Stéphane |
| author_facet | Pieretti Isabelle Royer Monique Barbe Valérie Carrere Sébastien Koebnik Ralf Couloux Arnaud Darrasse Armelle Gouzy Jérôme Jacques Marie-Agnès Lauber Emmanuelle Manceau Charles Mangenot Sophie Poussier Stéphane Segurens Béatrice Szurek Boris Verdier Valérie Arlat Matthieu Gabriel Dean W Rott Philippe Cociancich Stéphane |
| author_sort | Pieretti Isabelle |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Xanthomonas albilineans</it> causes leaf scald, a lethal disease of sugarcane. <it>X. albilineans</it> exhibits distinctive pathogenic mechanisms, ecology and taxonomy compared to other species of <it>Xanthomonas</it>. For example, this species produces a potent DNA gyrase inhibitor called albicidin that is largely responsible for inducing disease symptoms; its habitat is limited to xylem; and the species exhibits large variability. A first manuscript on the complete genome sequence of the highly pathogenic <it>X. albilineans</it> strain GPE PC73 focused exclusively on distinctive genomic features shared with <it>Xylella fastidiosa</it>—another xylem-limited <it>Xanthomonadaceae</it>. The present manuscript on the same genome sequence aims to describe all other pathogenicity-related genomic features of <it>X. albilineans</it>, and to compare, using suppression subtractive hybridization (SSH), genomic features of two strains differing in pathogenicity.</p> <p>Results</p> <p>Comparative genomic analyses showed that most of the known pathogenicity factors from other <it>Xanthomonas</it> species are conserved in <it>X. albilineans</it>, with the notable absence of two major determinants of the “artillery” of other plant pathogenic species of <it>Xanthomonas</it>: the xanthan gum biosynthesis gene cluster, and the type III secretion system Hrp (hypersensitive response and pathogenicity). Genomic features specific to <it>X. albilineans</it> that may contribute to specific adaptation of this pathogen to sugarcane xylem vessels were also revealed. SSH experiments led to the identification of 20 genes common to three highly pathogenic strains but missing in a less pathogenic strain. These 20 genes, which include four ABC transporter genes, a methyl-accepting chemotaxis protein gene and an oxidoreductase gene, could play a key role in pathogenicity. With the exception of hypothetical proteins revealed by our comparative genomic analyses and SSH experiments, no genes potentially involved in any offensive or counter-defensive mechanism specific to <it>X. albilineans</it> were identified, supposing that <it>X. albilineans</it> has a reduced artillery compared to other pathogenic <it>Xanthomonas</it> species. Particular attention has therefore been given to genomic features specific to <it>X. albilineans</it> making it more capable of evading sugarcane surveillance systems or resisting sugarcane defense systems.</p> <p>Conclusions</p> <p>This study confirms that <it>X. albilineans</it> is a highly distinctive species within the genus <it>Xanthomonas</it>, and opens new perpectives towards a greater understanding of the pathogenicity of this destructive sugarcane pathogen.</p> |
| first_indexed | 2024-04-12T16:09:11Z |
| format | Article |
| id | doaj.art-a53b3f4e96f6484f8f2b3ebbc9c3aef0 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-12T16:09:11Z |
| publishDate | 2012-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-a53b3f4e96f6484f8f2b3ebbc9c3aef02022-12-22T03:25:57ZengBMCBMC Genomics1471-21642012-11-0113165810.1186/1471-2164-13-658Genomic insights into strategies used by <it>Xanthomonas albilineans</it> with its reduced artillery to spread within sugarcane xylem vesselsPieretti IsabelleRoyer MoniqueBarbe ValérieCarrere SébastienKoebnik RalfCouloux ArnaudDarrasse ArmelleGouzy JérômeJacques Marie-AgnèsLauber EmmanuelleManceau CharlesMangenot SophiePoussier StéphaneSegurens BéatriceSzurek BorisVerdier ValérieArlat MatthieuGabriel Dean WRott PhilippeCociancich Stéphane<p>Abstract</p> <p>Background</p> <p><it>Xanthomonas albilineans</it> causes leaf scald, a lethal disease of sugarcane. <it>X. albilineans</it> exhibits distinctive pathogenic mechanisms, ecology and taxonomy compared to other species of <it>Xanthomonas</it>. For example, this species produces a potent DNA gyrase inhibitor called albicidin that is largely responsible for inducing disease symptoms; its habitat is limited to xylem; and the species exhibits large variability. A first manuscript on the complete genome sequence of the highly pathogenic <it>X. albilineans</it> strain GPE PC73 focused exclusively on distinctive genomic features shared with <it>Xylella fastidiosa</it>—another xylem-limited <it>Xanthomonadaceae</it>. The present manuscript on the same genome sequence aims to describe all other pathogenicity-related genomic features of <it>X. albilineans</it>, and to compare, using suppression subtractive hybridization (SSH), genomic features of two strains differing in pathogenicity.</p> <p>Results</p> <p>Comparative genomic analyses showed that most of the known pathogenicity factors from other <it>Xanthomonas</it> species are conserved in <it>X. albilineans</it>, with the notable absence of two major determinants of the “artillery” of other plant pathogenic species of <it>Xanthomonas</it>: the xanthan gum biosynthesis gene cluster, and the type III secretion system Hrp (hypersensitive response and pathogenicity). Genomic features specific to <it>X. albilineans</it> that may contribute to specific adaptation of this pathogen to sugarcane xylem vessels were also revealed. SSH experiments led to the identification of 20 genes common to three highly pathogenic strains but missing in a less pathogenic strain. These 20 genes, which include four ABC transporter genes, a methyl-accepting chemotaxis protein gene and an oxidoreductase gene, could play a key role in pathogenicity. With the exception of hypothetical proteins revealed by our comparative genomic analyses and SSH experiments, no genes potentially involved in any offensive or counter-defensive mechanism specific to <it>X. albilineans</it> were identified, supposing that <it>X. albilineans</it> has a reduced artillery compared to other pathogenic <it>Xanthomonas</it> species. Particular attention has therefore been given to genomic features specific to <it>X. albilineans</it> making it more capable of evading sugarcane surveillance systems or resisting sugarcane defense systems.</p> <p>Conclusions</p> <p>This study confirms that <it>X. albilineans</it> is a highly distinctive species within the genus <it>Xanthomonas</it>, and opens new perpectives towards a greater understanding of the pathogenicity of this destructive sugarcane pathogen.</p>http://www.biomedcentral.com/1471-2164/13/658 |
| spellingShingle | Pieretti Isabelle Royer Monique Barbe Valérie Carrere Sébastien Koebnik Ralf Couloux Arnaud Darrasse Armelle Gouzy Jérôme Jacques Marie-Agnès Lauber Emmanuelle Manceau Charles Mangenot Sophie Poussier Stéphane Segurens Béatrice Szurek Boris Verdier Valérie Arlat Matthieu Gabriel Dean W Rott Philippe Cociancich Stéphane Genomic insights into strategies used by <it>Xanthomonas albilineans</it> with its reduced artillery to spread within sugarcane xylem vessels BMC Genomics |
| title | Genomic insights into strategies used by <it>Xanthomonas albilineans</it> with its reduced artillery to spread within sugarcane xylem vessels |
| title_full | Genomic insights into strategies used by <it>Xanthomonas albilineans</it> with its reduced artillery to spread within sugarcane xylem vessels |
| title_fullStr | Genomic insights into strategies used by <it>Xanthomonas albilineans</it> with its reduced artillery to spread within sugarcane xylem vessels |
| title_full_unstemmed | Genomic insights into strategies used by <it>Xanthomonas albilineans</it> with its reduced artillery to spread within sugarcane xylem vessels |
| title_short | Genomic insights into strategies used by <it>Xanthomonas albilineans</it> with its reduced artillery to spread within sugarcane xylem vessels |
| title_sort | genomic insights into strategies used by it xanthomonas albilineans it with its reduced artillery to spread within sugarcane xylem vessels |
| url | http://www.biomedcentral.com/1471-2164/13/658 |
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