Visceral adipose tissue visfatin in nonalcoholic fatty liver disease
Background. Visfatin is a novel adipocytokine predominantly expressed and secreted by visceral adipose tissue. It is realized for its multiple functions of central importance in NAD biosynthesis, innate immunity and inflammation. Its phosphoribosyl transferase activity regulates cellular energetics...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2010-07-01
|
| Series: | Annals of Hepatology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1665268119316357 |
| _version_ | 1818607761328439296 |
|---|---|
| author | Radhika Gaddipati Sasikala Mitnala, PhD Nagarajarao Padaki Rathindra Mohan Mukherjee Anuradha Sekaran Magnus Jayaraj-Mansard Pradeep Rabella Venkat Rao-Guduru Nageshwar Reddy-Duwuru |
| author_facet | Radhika Gaddipati Sasikala Mitnala, PhD Nagarajarao Padaki Rathindra Mohan Mukherjee Anuradha Sekaran Magnus Jayaraj-Mansard Pradeep Rabella Venkat Rao-Guduru Nageshwar Reddy-Duwuru |
| author_sort | Radhika Gaddipati |
| collection | DOAJ |
| description | Background. Visfatin is a novel adipocytokine predominantly expressed and secreted by visceral adipose tissue. It is realized for its multiple functions of central importance in NAD biosynthesis, innate immunity and inflammation. Its phosphoribosyl transferase activity regulates cellular energetics and NAD dependent enzymes such as SIRTUINS. Although its expression in various tissues and circulating levels are documented, visceral visfatin levels in Nonalcoholic fatty liver disease (NAFLD) patients have not been reported.Objective. The aim of the present study was to assess visceral adipose tissue visfatin levels in NAFLD.Materials and methods. A total of 115 patients undergoing diagnostic laparoscopy were recruited in the study and categorized into two groups based on standard criteria for NAFLD. Visceral adipose tissue TNF-α, IL-6 and visfatin levels were measured by ELISA. Blood glucose, lipids, liver enzymes and non esterified fatty acids (NEFA) were estimated using standard procedures. Formalin fixed, Hematoxylene Eosin stained liver biopsy specimens were examined for the presence of steatosis and the degree of steatosis was ascertained as per Brunt’s classification.Results. The visceral visfatin level declined significantly (P < 0.001) in all groups of NAFLD as compared to non NAFLD group, while plasma NEFA level increased with progressive steatosis (P < 0.02). Significant increase in TNF a was observed in all groups of NAFLD, while IL-6 increased in NASH only.Conclusion. A significant decline in visceral adipose tissue visfatin level was found to be associated with degree of steatosis in NAFLD patients. |
| first_indexed | 2024-12-16T14:31:53Z |
| format | Article |
| id | doaj.art-a53c971602694d27b101f34cf55553df |
| institution | Directory Open Access Journal |
| issn | 1665-2681 |
| language | English |
| last_indexed | 2024-12-16T14:31:53Z |
| publishDate | 2010-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Annals of Hepatology |
| spelling | doaj.art-a53c971602694d27b101f34cf55553df2022-12-21T22:28:13ZengElsevierAnnals of Hepatology1665-26812010-07-0193266270Visceral adipose tissue visfatin in nonalcoholic fatty liver diseaseRadhika Gaddipati0Sasikala Mitnala, PhD1Nagarajarao Padaki2Rathindra Mohan Mukherjee3Anuradha Sekaran4Magnus Jayaraj-Mansard5Pradeep Rabella6Venkat Rao-Guduru7Nageshwar Reddy-Duwuru8Institute of Basic Sciences and Translational Research, Asian Health Care FoundationInstitute of Basic Sciences and Translational Research, Asian Health Care Foundation; Correspondence and reprint request:Asian Institute of GastroenterologyInstitute of Basic Sciences and Translational Research, Asian Health Care FoundationAsian Institute of GastroenterologyAsian Institute of GastroenterologyAsian Institute of GastroenterologyAsian Institute of GastroenterologyAsian Institute of GastroenterologyBackground. Visfatin is a novel adipocytokine predominantly expressed and secreted by visceral adipose tissue. It is realized for its multiple functions of central importance in NAD biosynthesis, innate immunity and inflammation. Its phosphoribosyl transferase activity regulates cellular energetics and NAD dependent enzymes such as SIRTUINS. Although its expression in various tissues and circulating levels are documented, visceral visfatin levels in Nonalcoholic fatty liver disease (NAFLD) patients have not been reported.Objective. The aim of the present study was to assess visceral adipose tissue visfatin levels in NAFLD.Materials and methods. A total of 115 patients undergoing diagnostic laparoscopy were recruited in the study and categorized into two groups based on standard criteria for NAFLD. Visceral adipose tissue TNF-α, IL-6 and visfatin levels were measured by ELISA. Blood glucose, lipids, liver enzymes and non esterified fatty acids (NEFA) were estimated using standard procedures. Formalin fixed, Hematoxylene Eosin stained liver biopsy specimens were examined for the presence of steatosis and the degree of steatosis was ascertained as per Brunt’s classification.Results. The visceral visfatin level declined significantly (P < 0.001) in all groups of NAFLD as compared to non NAFLD group, while plasma NEFA level increased with progressive steatosis (P < 0.02). Significant increase in TNF a was observed in all groups of NAFLD, while IL-6 increased in NASH only.Conclusion. A significant decline in visceral adipose tissue visfatin level was found to be associated with degree of steatosis in NAFLD patients.http://www.sciencedirect.com/science/article/pii/S1665268119316357NAFLDTNF αNEFAVisceral visfatin |
| spellingShingle | Radhika Gaddipati Sasikala Mitnala, PhD Nagarajarao Padaki Rathindra Mohan Mukherjee Anuradha Sekaran Magnus Jayaraj-Mansard Pradeep Rabella Venkat Rao-Guduru Nageshwar Reddy-Duwuru Visceral adipose tissue visfatin in nonalcoholic fatty liver disease Annals of Hepatology NAFLD TNF α NEFA Visceral visfatin |
| title | Visceral adipose tissue visfatin in nonalcoholic fatty liver disease |
| title_full | Visceral adipose tissue visfatin in nonalcoholic fatty liver disease |
| title_fullStr | Visceral adipose tissue visfatin in nonalcoholic fatty liver disease |
| title_full_unstemmed | Visceral adipose tissue visfatin in nonalcoholic fatty liver disease |
| title_short | Visceral adipose tissue visfatin in nonalcoholic fatty liver disease |
| title_sort | visceral adipose tissue visfatin in nonalcoholic fatty liver disease |
| topic | NAFLD TNF α NEFA Visceral visfatin |
| url | http://www.sciencedirect.com/science/article/pii/S1665268119316357 |
| work_keys_str_mv | AT radhikagaddipati visceraladiposetissuevisfatininnonalcoholicfattyliverdisease AT sasikalamitnalaphd visceraladiposetissuevisfatininnonalcoholicfattyliverdisease AT nagarajaraopadaki visceraladiposetissuevisfatininnonalcoholicfattyliverdisease AT rathindramohanmukherjee visceraladiposetissuevisfatininnonalcoholicfattyliverdisease AT anuradhasekaran visceraladiposetissuevisfatininnonalcoholicfattyliverdisease AT magnusjayarajmansard visceraladiposetissuevisfatininnonalcoholicfattyliverdisease AT pradeeprabella visceraladiposetissuevisfatininnonalcoholicfattyliverdisease AT venkatraoguduru visceraladiposetissuevisfatininnonalcoholicfattyliverdisease AT nageshwarreddyduwuru visceraladiposetissuevisfatininnonalcoholicfattyliverdisease |