Heterologous Expression of a Ferritin Homologue Gene <i>PpFer1</i> from <i>Prunus persica</i> Enhances Plant Tolerance to Iron Toxicity and H<sub>2</sub>O<sub>2</sub> Stress in <i>Arabidopsis thaliana</i>
In plants, ferritin proteins play an important role in iron (Fe) storage which contributes to plant growth and development. However, the biological functions of ferritins in fruit trees are essentially unknown. In this study, three <i>Ferritin</i> genes were isolated from ‘Zhentong No. 3...
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MDPI AG
2023-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/24/4093 |
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| author | Yong Yang Jinjin Zhang Mengyuan Li Youzheng Ning Yifei Tao Shengpeng Shi Adeeba Dark Zhizhong Song |
| author_facet | Yong Yang Jinjin Zhang Mengyuan Li Youzheng Ning Yifei Tao Shengpeng Shi Adeeba Dark Zhizhong Song |
| author_sort | Yong Yang |
| collection | DOAJ |
| description | In plants, ferritin proteins play an important role in iron (Fe) storage which contributes to plant growth and development. However, the biological functions of ferritins in fruit trees are essentially unknown. In this study, three <i>Ferritin</i> genes were isolated from ‘Zhentong No. 3’ peach, which were named <i>PpFer1-PpFer3</i>. The expression levels of these genes were different in distinct tissues/organs. Notably, <i>PpFer1</i> was the most abundantly expressed Ferritin family gene in all tested tissues of ‘Zhentong No. 3’ peach; its expression levels were significantly enhanced throughout the entire peach seedling under Fe toxicity and H<sub>2</sub>O<sub>2</sub> stress, particularly in the leaves. In addition, over-expression of <i>PpFer1</i> was effective in rescuing the retarded growth of <i>Arabidopsis fer1-2</i> knockout mutant, embodied in enhanced fresh weight, primary root length, lateral root numbers, total root length, total leaf chlorophyll, stomatal conductance (<i>G</i><sub>s</sub>), net photosynthetic rate (<i>P</i><sub>n</sub>), transpiration rate, and tissue Fe concentration. This study provides insights into understanding the molecular mechanisms of Fe storage and sequestration in perennial fruit trees. |
| first_indexed | 2024-03-08T18:35:03Z |
| format | Article |
| id | doaj.art-a53ccbe9f5d8471b85877f90d78703ff |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-08T18:35:03Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-a53ccbe9f5d8471b85877f90d78703ff2023-12-29T15:47:20ZengMDPI AGPlants2223-77472023-12-011224409310.3390/plants12244093Heterologous Expression of a Ferritin Homologue Gene <i>PpFer1</i> from <i>Prunus persica</i> Enhances Plant Tolerance to Iron Toxicity and H<sub>2</sub>O<sub>2</sub> Stress in <i>Arabidopsis thaliana</i>Yong Yang0Jinjin Zhang1Mengyuan Li2Youzheng Ning3Yifei Tao4Shengpeng Shi5Adeeba Dark6Zhizhong Song7Zhenjiang Academy of Agricultural Sciences, Zhenjiang Institute of Agricultural Sciences in Hilly Areas of Jiangsu Province, Zhenjiang 212400, ChinaFaculty of Modern Agriculture, Linyi Vocational University of Science and Technology, No. 1 Macau Road, Linyi 276000, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, No. 186 Hongqizhong Road, Yantai 264025, ChinaDepartment of Plant Science, University of Cambridge, Cambridge CB2 3EA, UKThe Engineering Research Institute of Agriculture and Forestry, Ludong University, No. 186 Hongqizhong Road, Yantai 264025, ChinaDepartment of Plant Science, University of Cambridge, Cambridge CB2 3EA, UKDepartment of Plant Science, University of Cambridge, Cambridge CB2 3EA, UKThe Engineering Research Institute of Agriculture and Forestry, Ludong University, No. 186 Hongqizhong Road, Yantai 264025, ChinaIn plants, ferritin proteins play an important role in iron (Fe) storage which contributes to plant growth and development. However, the biological functions of ferritins in fruit trees are essentially unknown. In this study, three <i>Ferritin</i> genes were isolated from ‘Zhentong No. 3’ peach, which were named <i>PpFer1-PpFer3</i>. The expression levels of these genes were different in distinct tissues/organs. Notably, <i>PpFer1</i> was the most abundantly expressed Ferritin family gene in all tested tissues of ‘Zhentong No. 3’ peach; its expression levels were significantly enhanced throughout the entire peach seedling under Fe toxicity and H<sub>2</sub>O<sub>2</sub> stress, particularly in the leaves. In addition, over-expression of <i>PpFer1</i> was effective in rescuing the retarded growth of <i>Arabidopsis fer1-2</i> knockout mutant, embodied in enhanced fresh weight, primary root length, lateral root numbers, total root length, total leaf chlorophyll, stomatal conductance (<i>G</i><sub>s</sub>), net photosynthetic rate (<i>P</i><sub>n</sub>), transpiration rate, and tissue Fe concentration. This study provides insights into understanding the molecular mechanisms of Fe storage and sequestration in perennial fruit trees.https://www.mdpi.com/2223-7747/12/24/4093peachFe storageferritinFe toxicityH<sub>2</sub>O<sub>2</sub> stress |
| spellingShingle | Yong Yang Jinjin Zhang Mengyuan Li Youzheng Ning Yifei Tao Shengpeng Shi Adeeba Dark Zhizhong Song Heterologous Expression of a Ferritin Homologue Gene <i>PpFer1</i> from <i>Prunus persica</i> Enhances Plant Tolerance to Iron Toxicity and H<sub>2</sub>O<sub>2</sub> Stress in <i>Arabidopsis thaliana</i> Plants peach Fe storage ferritin Fe toxicity H<sub>2</sub>O<sub>2</sub> stress |
| title | Heterologous Expression of a Ferritin Homologue Gene <i>PpFer1</i> from <i>Prunus persica</i> Enhances Plant Tolerance to Iron Toxicity and H<sub>2</sub>O<sub>2</sub> Stress in <i>Arabidopsis thaliana</i> |
| title_full | Heterologous Expression of a Ferritin Homologue Gene <i>PpFer1</i> from <i>Prunus persica</i> Enhances Plant Tolerance to Iron Toxicity and H<sub>2</sub>O<sub>2</sub> Stress in <i>Arabidopsis thaliana</i> |
| title_fullStr | Heterologous Expression of a Ferritin Homologue Gene <i>PpFer1</i> from <i>Prunus persica</i> Enhances Plant Tolerance to Iron Toxicity and H<sub>2</sub>O<sub>2</sub> Stress in <i>Arabidopsis thaliana</i> |
| title_full_unstemmed | Heterologous Expression of a Ferritin Homologue Gene <i>PpFer1</i> from <i>Prunus persica</i> Enhances Plant Tolerance to Iron Toxicity and H<sub>2</sub>O<sub>2</sub> Stress in <i>Arabidopsis thaliana</i> |
| title_short | Heterologous Expression of a Ferritin Homologue Gene <i>PpFer1</i> from <i>Prunus persica</i> Enhances Plant Tolerance to Iron Toxicity and H<sub>2</sub>O<sub>2</sub> Stress in <i>Arabidopsis thaliana</i> |
| title_sort | heterologous expression of a ferritin homologue gene i ppfer1 i from i prunus persica i enhances plant tolerance to iron toxicity and h sub 2 sub o sub 2 sub stress in i arabidopsis thaliana i |
| topic | peach Fe storage ferritin Fe toxicity H<sub>2</sub>O<sub>2</sub> stress |
| url | https://www.mdpi.com/2223-7747/12/24/4093 |
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