A click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samples
Accurate detection of antigen p24 for HIV−1 early diagnosis remains challenging. Here the authors present a click chemistry amplified nanopore (CAN) assay that allows p24 quantification at ultralow concentration range in clinical samples.
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-34273-x |
| _version_ | 1828330355090784256 |
|---|---|
| author | Xiaojun Wei Xiaoqin Wang Zehui Zhang Yuanyuan Luo Zixin Wang Wen Xiong Piyush K. Jain John R. Monnier Hui Wang Tony Y. Hu Chuanbing Tang Helmut Albrecht Chang Liu |
| author_facet | Xiaojun Wei Xiaoqin Wang Zehui Zhang Yuanyuan Luo Zixin Wang Wen Xiong Piyush K. Jain John R. Monnier Hui Wang Tony Y. Hu Chuanbing Tang Helmut Albrecht Chang Liu |
| author_sort | Xiaojun Wei |
| collection | DOAJ |
| description | Accurate detection of antigen p24 for HIV−1 early diagnosis remains challenging. Here the authors present a click chemistry amplified nanopore (CAN) assay that allows p24 quantification at ultralow concentration range in clinical samples. |
| first_indexed | 2024-04-13T20:39:09Z |
| format | Article |
| id | doaj.art-a53cd8defb0a4df3886cc8a157c25efb |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-13T20:39:09Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-a53cd8defb0a4df3886cc8a157c25efb2022-12-22T02:30:57ZengNature PortfolioNature Communications2041-17232022-11-0113111310.1038/s41467-022-34273-xA click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samplesXiaojun Wei0Xiaoqin Wang1Zehui Zhang2Yuanyuan Luo3Zixin Wang4Wen Xiong5Piyush K. Jain6John R. Monnier7Hui Wang8Tony Y. Hu9Chuanbing Tang10Helmut Albrecht11Chang Liu12Biomedical Engineering Program, University of South CarolinaDepartment of Chemical Engineering, University of South CarolinaBiomedical Engineering Program, University of South CarolinaDepartment of Chemistry and Biochemistry, University of South CarolinaDepartment of Chemistry and Biochemistry, University of South CarolinaDepartment of Chemical Engineering, University of South CarolinaDepartment of Chemical Engineering, University of FloridaDepartment of Chemical Engineering, University of South CarolinaDepartment of Chemistry and Biochemistry, University of South CarolinaCenter for Cellular and Molecular Diagnostics, Tulane University School of MedicineDepartment of Chemistry and Biochemistry, University of South CarolinaDepartment of Internal Medicine, School of Medicine, University of South CarolinaBiomedical Engineering Program, University of South CarolinaAccurate detection of antigen p24 for HIV−1 early diagnosis remains challenging. Here the authors present a click chemistry amplified nanopore (CAN) assay that allows p24 quantification at ultralow concentration range in clinical samples.https://doi.org/10.1038/s41467-022-34273-x |
| spellingShingle | Xiaojun Wei Xiaoqin Wang Zehui Zhang Yuanyuan Luo Zixin Wang Wen Xiong Piyush K. Jain John R. Monnier Hui Wang Tony Y. Hu Chuanbing Tang Helmut Albrecht Chang Liu A click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samples Nature Communications |
| title | A click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samples |
| title_full | A click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samples |
| title_fullStr | A click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samples |
| title_full_unstemmed | A click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samples |
| title_short | A click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samples |
| title_sort | click chemistry amplified nanopore assay for ultrasensitive quantification of hiv 1 p24 antigen in clinical samples |
| url | https://doi.org/10.1038/s41467-022-34273-x |
| work_keys_str_mv | AT xiaojunwei aclickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT xiaoqinwang aclickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT zehuizhang aclickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT yuanyuanluo aclickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT zixinwang aclickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT wenxiong aclickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT piyushkjain aclickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT johnrmonnier aclickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT huiwang aclickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT tonyyhu aclickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT chuanbingtang aclickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT helmutalbrecht aclickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT changliu aclickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT xiaojunwei clickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT xiaoqinwang clickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT zehuizhang clickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT yuanyuanluo clickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT zixinwang clickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT wenxiong clickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT piyushkjain clickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT johnrmonnier clickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT huiwang clickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT tonyyhu clickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT chuanbingtang clickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT helmutalbrecht clickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples AT changliu clickchemistryamplifiednanoporeassayforultrasensitivequantificationofhiv1p24antigeninclinicalsamples |