Сontent of cytokines and growth factors in the intraocular fluid of patients with primary open-angle glaucoma
Purpose. To study the content of cytokines and growth factors in the intraocular fluid of patients with developed stage of primary open-angle glaucoma (POAG).Materials and methods. 56 patients with a verified diagnosis developed stage of primary open-angle glaucomawere examined. The control group co...
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| Format: | Article |
| Language: | English |
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Siberian State Medical University (Tomsk)
2019-05-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/2192 |
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| author | V. V. Chernykh V. I. Konenkov O. V. Ermakova N. B. Orlov O. O. Obukhova A. V. Eremina A. N. Trunov |
| author_facet | V. V. Chernykh V. I. Konenkov O. V. Ermakova N. B. Orlov O. O. Obukhova A. V. Eremina A. N. Trunov |
| author_sort | V. V. Chernykh |
| collection | DOAJ |
| description | Purpose. To study the content of cytokines and growth factors in the intraocular fluid of patients with developed stage of primary open-angle glaucoma (POAG).Materials and methods. 56 patients with a verified diagnosis developed stage of primary open-angle glaucomawere examined. The control group consisted of 30 patients with a diagnosis of uncomplicated cataract. A concentration of 17 cytokinesand 3 isoforms of the transforming growth factor (TGF) β was determined using a Bio-Plex Pro™ Human Cytokine 17-plex Assay and Bio-Plex Pro™ and TGFβ 3-plex Assay kit by flow-through fluorometry on a Bio-Plex 200, Bio-Rad double beam laser analyzer, USA.Results. Astatistically significant increase was shown in the concentrations of cytokines and growth factors (interleukins (IL) 4, 6, 7, 8, 12, 17, TGFβ 1, 2, 3, macrophage inflammatory protein 1 β) in the intraocular fluid of patients with developed stage of the primary open-angle glaucoma in respect to data obtained from the study of the intraocular fluid of the persons with uncomplicated cataract, as well as a statistically significant decrease in the concentrations IL-2, IL-10, granulocyte-macrophage colony-stimulating factor.Conclusion. In the pathogenesis of primary open-angle glaucoma, the activity of the local chronic inflammatory process is determined. This is confirmed by statistically significant changes in the studied cytokines and growth factors. Increase in the concentrations of the studied representatives of the superfamily of transforming growth factors-beta, which have anti-inflammatory activity, the ability to stimulate proliferation, cell growth, synthesis of extracellular matrix proteins, etc., attests to their importance in the mechanisms of primary open-angle glaucoma development. Increase concentrations of IL-7 in the intraocular fluid of patients with primary open-angle glaucoma allows us to assume participation in the pathogenesis of the primary open-angle glaucoma of this autocrine mediator of activation of the growth of lymphatic structures. |
| first_indexed | 2024-04-10T01:06:39Z |
| format | Article |
| id | doaj.art-a53f083538f3441481103ac97fc899f9 |
| institution | Directory Open Access Journal |
| issn | 1682-0363 1819-3684 |
| language | English |
| last_indexed | 2024-04-10T01:06:39Z |
| publishDate | 2019-05-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj.art-a53f083538f3441481103ac97fc899f92023-03-13T09:58:24ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842019-05-0118125726510.20538/1682-0363-2019-1-257-2651499Сontent of cytokines and growth factors in the intraocular fluid of patients with primary open-angle glaucomaV. V. Chernykh0V. I. Konenkov1O. V. Ermakova2N. B. Orlov3O. O. Obukhova4A. V. Eremina5A. N. Trunov6Национальный медицинский исследовательский центр (НМИЦ) «МНТК «Микрохирургия глаза» им. акад. С.Н. Федорова МЗ РФ»НИИ клинической и экспериментальной лимфологии (НИИКЭЛ) – филиал Федерального исследовательского центра «Институт цитологии и генетики Сибирского отделения Российской академии наук» (ФИЦ «ИЦиГ СО РАН»)Национальный медицинский исследовательский центр (НМИЦ) «МНТК «Микрохирургия глаза» им. акад. С.Н. Федорова МЗ РФ»НИИ клинической и экспериментальной лимфологии (НИИКЭЛ) – филиал Федерального исследовательского центра «Институт цитологии и генетики Сибирского отделения Российской академии наук» (ФИЦ «ИЦиГ СО РАН»)Федеральный исследовательский центр фундаментальной и трансляционной медицины Сибирского отделения Российской академии наук (ФИЦ ФТМ СО РАН)НИИ клинической и экспериментальной лимфологии (НИИКЭЛ) – филиал Федерального исследовательского центра «Институт цитологии и генетики Сибирского отделения Российской академии наук» (ФИЦ «ИЦиГ СО РАН»)Национальный медицинский исследовательский центр (НМИЦ) «МНТК «Микрохирургия глаза» им. акад. С.Н. Федорова МЗ РФ»; Федеральный исследовательский центр фундаментальной и трансляционной медицины Сибирского отделения Российской академии наук (ФИЦ ФТМ СО РАН)Purpose. To study the content of cytokines and growth factors in the intraocular fluid of patients with developed stage of primary open-angle glaucoma (POAG).Materials and methods. 56 patients with a verified diagnosis developed stage of primary open-angle glaucomawere examined. The control group consisted of 30 patients with a diagnosis of uncomplicated cataract. A concentration of 17 cytokinesand 3 isoforms of the transforming growth factor (TGF) β was determined using a Bio-Plex Pro™ Human Cytokine 17-plex Assay and Bio-Plex Pro™ and TGFβ 3-plex Assay kit by flow-through fluorometry on a Bio-Plex 200, Bio-Rad double beam laser analyzer, USA.Results. Astatistically significant increase was shown in the concentrations of cytokines and growth factors (interleukins (IL) 4, 6, 7, 8, 12, 17, TGFβ 1, 2, 3, macrophage inflammatory protein 1 β) in the intraocular fluid of patients with developed stage of the primary open-angle glaucoma in respect to data obtained from the study of the intraocular fluid of the persons with uncomplicated cataract, as well as a statistically significant decrease in the concentrations IL-2, IL-10, granulocyte-macrophage colony-stimulating factor.Conclusion. In the pathogenesis of primary open-angle glaucoma, the activity of the local chronic inflammatory process is determined. This is confirmed by statistically significant changes in the studied cytokines and growth factors. Increase in the concentrations of the studied representatives of the superfamily of transforming growth factors-beta, which have anti-inflammatory activity, the ability to stimulate proliferation, cell growth, synthesis of extracellular matrix proteins, etc., attests to their importance in the mechanisms of primary open-angle glaucoma development. Increase concentrations of IL-7 in the intraocular fluid of patients with primary open-angle glaucoma allows us to assume participation in the pathogenesis of the primary open-angle glaucoma of this autocrine mediator of activation of the growth of lymphatic structures.https://bulletin.ssmu.ru/jour/article/view/2192первичная открытоугольная глаукомапатогенезвоспалениецитокинытрансформирующие факторы роставнутриглазная жидкость |
| spellingShingle | V. V. Chernykh V. I. Konenkov O. V. Ermakova N. B. Orlov O. O. Obukhova A. V. Eremina A. N. Trunov Сontent of cytokines and growth factors in the intraocular fluid of patients with primary open-angle glaucoma Бюллетень сибирской медицины первичная открытоугольная глаукома патогенез воспаление цитокины трансформирующие факторы роста внутриглазная жидкость |
| title | Сontent of cytokines and growth factors in the intraocular fluid of patients with primary open-angle glaucoma |
| title_full | Сontent of cytokines and growth factors in the intraocular fluid of patients with primary open-angle glaucoma |
| title_fullStr | Сontent of cytokines and growth factors in the intraocular fluid of patients with primary open-angle glaucoma |
| title_full_unstemmed | Сontent of cytokines and growth factors in the intraocular fluid of patients with primary open-angle glaucoma |
| title_short | Сontent of cytokines and growth factors in the intraocular fluid of patients with primary open-angle glaucoma |
| title_sort | сontent of cytokines and growth factors in the intraocular fluid of patients with primary open angle glaucoma |
| topic | первичная открытоугольная глаукома патогенез воспаление цитокины трансформирующие факторы роста внутриглазная жидкость |
| url | https://bulletin.ssmu.ru/jour/article/view/2192 |
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