Assessment of human adipose‐derived stem cell on surface‐modified silicone implant to reduce capsular contracture formation
Abstract Medical devices made from poly(dimethylsiloxane) (PDMS)‐based silicone implants have been broadly used owing to their inert properties, biocompatibility, and low toxicity. However, long‐term implantation is usually associated with complications, such as capsular contracture due to excessive...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Bioengineering & Translational Medicine |
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| Online Access: | https://doi.org/10.1002/btm2.10260 |
| _version_ | 1798016074820616192 |
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| author | Chanutchamon Sutthiwanjampa Byung Ho Shin Na Eun Ryu Shin Hyuk Kang Chan Yeong Heo Hansoo Park |
| author_facet | Chanutchamon Sutthiwanjampa Byung Ho Shin Na Eun Ryu Shin Hyuk Kang Chan Yeong Heo Hansoo Park |
| author_sort | Chanutchamon Sutthiwanjampa |
| collection | DOAJ |
| description | Abstract Medical devices made from poly(dimethylsiloxane) (PDMS)‐based silicone implants have been broadly used owing to their inert properties, biocompatibility, and low toxicity. However, long‐term implantation is usually associated with complications, such as capsular contracture due to excessive local inflammatory response, subsequently requiring implant removal. Therefore, modification of the silicone surface to reduce a risk of capsular contracture has attracted increasing attention. Human adipose‐derived stem cells (hASCs) are known to provide potentially therapeutic applications for tissue engineering, regenerative medicine, and reconstructive surgery. Herein, hASCs coating on a PDMS (hASC‐PDMS) or itaconic acid (IA)‐conjugated PDMS (hASC‐IA‐PDMS) surface is examined to determine its biocompatibility for reducing capsular contracture on the PDMS surface. In vitro cell cytotoxicity evaluation showed that hASCs on IA‐PDMS exhibit higher cell viability than hASCs on PDMS. A lower release of proinflammatory cytokines is observed in hASC‐PDMS and hASC‐IA‐PDMS compared to the cells on plate. Multiple factors, including in vivo mRNA expression levels of cytokines related to fibrosis; number of inflammatory cells; number of macrophages and myofibroblasts; capsule thickness; and collagen density following implantation in rats for 60 days, indicate that incorporated coating hASCs on PDMSs most effectively reduces capsular contracture. This study demonstrates the potential of hASCs coating for the modification of PDMS surfaces in enhancing surface biocompatibility for reducing capsular contracture of PDMS‐based medical devices. |
| first_indexed | 2024-04-11T15:44:59Z |
| format | Article |
| id | doaj.art-a541350cc4cb485cbb9571814fd65ee1 |
| institution | Directory Open Access Journal |
| issn | 2380-6761 |
| language | English |
| last_indexed | 2024-04-11T15:44:59Z |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj.art-a541350cc4cb485cbb9571814fd65ee12022-12-22T04:15:37ZengWileyBioengineering & Translational Medicine2380-67612022-01-0171n/an/a10.1002/btm2.10260Assessment of human adipose‐derived stem cell on surface‐modified silicone implant to reduce capsular contracture formationChanutchamon Sutthiwanjampa0Byung Ho Shin1Na Eun Ryu2Shin Hyuk Kang3Chan Yeong Heo4Hansoo Park5School of Integrative Engineering, Chung‐Ang University Seoul Republic of KoreaDepartment of Biomedical Engineering College of Medicine, Seoul National University Seoul Republic of KoreaSchool of Integrative Engineering, Chung‐Ang University Seoul Republic of KoreaDepartment of Plastic and Reconstructive Surgery Chung‐Ang University Hospital Seoul Republic of KoreaDepartment of Biomedical Engineering College of Medicine, Seoul National University Seoul Republic of KoreaSchool of Integrative Engineering, Chung‐Ang University Seoul Republic of KoreaAbstract Medical devices made from poly(dimethylsiloxane) (PDMS)‐based silicone implants have been broadly used owing to their inert properties, biocompatibility, and low toxicity. However, long‐term implantation is usually associated with complications, such as capsular contracture due to excessive local inflammatory response, subsequently requiring implant removal. Therefore, modification of the silicone surface to reduce a risk of capsular contracture has attracted increasing attention. Human adipose‐derived stem cells (hASCs) are known to provide potentially therapeutic applications for tissue engineering, regenerative medicine, and reconstructive surgery. Herein, hASCs coating on a PDMS (hASC‐PDMS) or itaconic acid (IA)‐conjugated PDMS (hASC‐IA‐PDMS) surface is examined to determine its biocompatibility for reducing capsular contracture on the PDMS surface. In vitro cell cytotoxicity evaluation showed that hASCs on IA‐PDMS exhibit higher cell viability than hASCs on PDMS. A lower release of proinflammatory cytokines is observed in hASC‐PDMS and hASC‐IA‐PDMS compared to the cells on plate. Multiple factors, including in vivo mRNA expression levels of cytokines related to fibrosis; number of inflammatory cells; number of macrophages and myofibroblasts; capsule thickness; and collagen density following implantation in rats for 60 days, indicate that incorporated coating hASCs on PDMSs most effectively reduces capsular contracture. This study demonstrates the potential of hASCs coating for the modification of PDMS surfaces in enhancing surface biocompatibility for reducing capsular contracture of PDMS‐based medical devices.https://doi.org/10.1002/btm2.10260antifibrotic propertycapsular contractureforeign body responsehuman adipose‐derived stem cellsitaconic acidpoly(dimethylsiloxane) |
| spellingShingle | Chanutchamon Sutthiwanjampa Byung Ho Shin Na Eun Ryu Shin Hyuk Kang Chan Yeong Heo Hansoo Park Assessment of human adipose‐derived stem cell on surface‐modified silicone implant to reduce capsular contracture formation Bioengineering & Translational Medicine antifibrotic property capsular contracture foreign body response human adipose‐derived stem cells itaconic acid poly(dimethylsiloxane) |
| title | Assessment of human adipose‐derived stem cell on surface‐modified silicone implant to reduce capsular contracture formation |
| title_full | Assessment of human adipose‐derived stem cell on surface‐modified silicone implant to reduce capsular contracture formation |
| title_fullStr | Assessment of human adipose‐derived stem cell on surface‐modified silicone implant to reduce capsular contracture formation |
| title_full_unstemmed | Assessment of human adipose‐derived stem cell on surface‐modified silicone implant to reduce capsular contracture formation |
| title_short | Assessment of human adipose‐derived stem cell on surface‐modified silicone implant to reduce capsular contracture formation |
| title_sort | assessment of human adipose derived stem cell on surface modified silicone implant to reduce capsular contracture formation |
| topic | antifibrotic property capsular contracture foreign body response human adipose‐derived stem cells itaconic acid poly(dimethylsiloxane) |
| url | https://doi.org/10.1002/btm2.10260 |
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