Eugenol improves high-fat diet/streptomycin-induced type 2 diabetes mellitus (T2DM) mice muscle dysfunction by alleviating inflammation and increasing muscle glucose uptake
Eugenol has been used in dietary interventions for metabolic diseases such as diabetes and obesity. However, the protective effect of eugenol on muscle function in diabetes is unclear. In this study, a high-fat diet (HFD) with a streptozocin (STZ) injection induced type II diabetes mellitus in a mou...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1039753/full |
| _version_ | 1811221964106235904 |
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| author | Yuge Jiang Yuge Jiang Chuanxing Feng Chuanxing Feng Yonghui Shi Yonghui Shi Xingran Kou Guowei Le Guowei Le |
| author_facet | Yuge Jiang Yuge Jiang Chuanxing Feng Chuanxing Feng Yonghui Shi Yonghui Shi Xingran Kou Guowei Le Guowei Le |
| author_sort | Yuge Jiang |
| collection | DOAJ |
| description | Eugenol has been used in dietary interventions for metabolic diseases such as diabetes and obesity. However, the protective effect of eugenol on muscle function in diabetes is unclear. In this study, a high-fat diet (HFD) with a streptozocin (STZ) injection induced type II diabetes mellitus in a mouse model. Oral eugenol lowered blood glucose and insulin resistance of HFD/STZ-treated mice. Eugenol reduced HFD/STZ-induced muscle inflammation and prevented muscle weakness and atrophy. Eugenol administration significantly increased GLUT4 translocation and AMPK phosphorylation in skeletal muscle, thereby enhancing glucose uptake. By silencing the transient receptor potential vanilloid channel 1 (TRPV1) gene in C2C12 myotube cells, eugenol was found to increase intracellular Ca2+ levels through TRPV1, which then activated calmodulin-dependent protein kinase-2 (CaMKK2) and affected AMPK protein phosphorylation. In conclusion, eugenol is a potential nutraceutical for preventing high-glucose-induced muscle impairments, which could be explained by its mediating effects on glucose absorption and inflammatory responses in the muscle. |
| first_indexed | 2024-04-12T08:09:07Z |
| format | Article |
| id | doaj.art-a541b4ba84774722a461191f2d6140c1 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-12T08:09:07Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-a541b4ba84774722a461191f2d6140c12022-12-22T03:41:01ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-11-01910.3389/fnut.2022.10397531039753Eugenol improves high-fat diet/streptomycin-induced type 2 diabetes mellitus (T2DM) mice muscle dysfunction by alleviating inflammation and increasing muscle glucose uptakeYuge Jiang0Yuge Jiang1Chuanxing Feng2Chuanxing Feng3Yonghui Shi4Yonghui Shi5Xingran Kou6Guowei Le7Guowei Le8Center for Food Nutrition and Functional Food Engineering, School of Food Science and Technology, Jiangnan University, Wuxi, ChinaThe State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, ChinaCenter for Food Nutrition and Functional Food Engineering, School of Food Science and Technology, Jiangnan University, Wuxi, ChinaThe State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, ChinaCenter for Food Nutrition and Functional Food Engineering, School of Food Science and Technology, Jiangnan University, Wuxi, ChinaThe State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, ChinaCenter for Food Nutrition and Functional Food Engineering, School of Food Science and Technology, Jiangnan University, Wuxi, ChinaThe State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, ChinaEugenol has been used in dietary interventions for metabolic diseases such as diabetes and obesity. However, the protective effect of eugenol on muscle function in diabetes is unclear. In this study, a high-fat diet (HFD) with a streptozocin (STZ) injection induced type II diabetes mellitus in a mouse model. Oral eugenol lowered blood glucose and insulin resistance of HFD/STZ-treated mice. Eugenol reduced HFD/STZ-induced muscle inflammation and prevented muscle weakness and atrophy. Eugenol administration significantly increased GLUT4 translocation and AMPK phosphorylation in skeletal muscle, thereby enhancing glucose uptake. By silencing the transient receptor potential vanilloid channel 1 (TRPV1) gene in C2C12 myotube cells, eugenol was found to increase intracellular Ca2+ levels through TRPV1, which then activated calmodulin-dependent protein kinase-2 (CaMKK2) and affected AMPK protein phosphorylation. In conclusion, eugenol is a potential nutraceutical for preventing high-glucose-induced muscle impairments, which could be explained by its mediating effects on glucose absorption and inflammatory responses in the muscle.https://www.frontiersin.org/articles/10.3389/fnut.2022.1039753/fulleugenoldiabetesglucosespicesmuscle |
| spellingShingle | Yuge Jiang Yuge Jiang Chuanxing Feng Chuanxing Feng Yonghui Shi Yonghui Shi Xingran Kou Guowei Le Guowei Le Eugenol improves high-fat diet/streptomycin-induced type 2 diabetes mellitus (T2DM) mice muscle dysfunction by alleviating inflammation and increasing muscle glucose uptake Frontiers in Nutrition eugenol diabetes glucose spices muscle |
| title | Eugenol improves high-fat diet/streptomycin-induced type 2 diabetes mellitus (T2DM) mice muscle dysfunction by alleviating inflammation and increasing muscle glucose uptake |
| title_full | Eugenol improves high-fat diet/streptomycin-induced type 2 diabetes mellitus (T2DM) mice muscle dysfunction by alleviating inflammation and increasing muscle glucose uptake |
| title_fullStr | Eugenol improves high-fat diet/streptomycin-induced type 2 diabetes mellitus (T2DM) mice muscle dysfunction by alleviating inflammation and increasing muscle glucose uptake |
| title_full_unstemmed | Eugenol improves high-fat diet/streptomycin-induced type 2 diabetes mellitus (T2DM) mice muscle dysfunction by alleviating inflammation and increasing muscle glucose uptake |
| title_short | Eugenol improves high-fat diet/streptomycin-induced type 2 diabetes mellitus (T2DM) mice muscle dysfunction by alleviating inflammation and increasing muscle glucose uptake |
| title_sort | eugenol improves high fat diet streptomycin induced type 2 diabetes mellitus t2dm mice muscle dysfunction by alleviating inflammation and increasing muscle glucose uptake |
| topic | eugenol diabetes glucose spices muscle |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1039753/full |
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