Applications and Prospects of CRISPR/Cas9-Mediated Base Editing in Plant Breeding
The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein 9 system (Cas9) has been used at length to optimize multiple aspects of germplasm resources. However, large-scale genomic research has indicated that novel variations in crop plants are attributed to single-nuc...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/2/59 |
| _version_ | 1797621666895888384 |
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| author | Yizhen Li Jing Liang Bufang Deng Yingli Jiang Jingyan Zhu Like Chen Min Li Juan Li |
| author_facet | Yizhen Li Jing Liang Bufang Deng Yingli Jiang Jingyan Zhu Like Chen Min Li Juan Li |
| author_sort | Yizhen Li |
| collection | DOAJ |
| description | The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein 9 system (Cas9) has been used at length to optimize multiple aspects of germplasm resources. However, large-scale genomic research has indicated that novel variations in crop plants are attributed to single-nucleotide polymorphisms (SNPs). Therefore, substituting single bases into a plant genome may produce desirable traits. Gene editing by CRISPR/Cas9 techniques frequently results in insertions–deletions (indels). Base editing allows precise single-nucleotide changes in the genome in the absence of double-strand breaks (DSBs) and donor repair templates (DRTs). Therefore, BEs have provided a new way of thinking about genome editing, and base editing techniques are currently being utilized to edit the genomes of many different organisms. As traditional breeding techniques and modern molecular breeding technologies complement each other, various genome editing technologies have emerged. How to realize the greater potential of BE applications is the question we need to consider. Here, we explain various base editings such as CBEs, ABEs, and CGBEs. In addition, the latest applications of base editing technologies in agriculture are summarized, including crop yield, quality, disease, and herbicide resistance. Finally, the challenges and future prospects of base editing technologies are presented. The aim is to provide a comprehensive overview of the application of BE in crop breeding to further improve BE and make the most of its value. |
| first_indexed | 2024-03-11T08:59:11Z |
| format | Article |
| id | doaj.art-a54596cd32d74a3a9fc0351a603dbe98 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-11T08:59:11Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-a54596cd32d74a3a9fc0351a603dbe982023-11-16T19:50:31ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-01-0145291893510.3390/cimb45020059Applications and Prospects of CRISPR/Cas9-Mediated Base Editing in Plant BreedingYizhen Li0Jing Liang1Bufang Deng2Yingli Jiang3Jingyan Zhu4Like Chen5Min Li6Juan Li7College of Agronomy, Anhui Agricultural University, Heifei 230036, ChinaCollege of Agronomy, Anhui Agricultural University, Heifei 230036, ChinaCollege of Agronomy, Anhui Agricultural University, Heifei 230036, ChinaCollege of Agronomy, Anhui Agricultural University, Heifei 230036, ChinaCollege of Agronomy, Anhui Agricultural University, Heifei 230036, ChinaCollege of Agronomy, Anhui Agricultural University, Heifei 230036, ChinaCollege of Agronomy, Anhui Agricultural University, Heifei 230036, ChinaKey Laboratory of Rice Genetic Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaThe clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein 9 system (Cas9) has been used at length to optimize multiple aspects of germplasm resources. However, large-scale genomic research has indicated that novel variations in crop plants are attributed to single-nucleotide polymorphisms (SNPs). Therefore, substituting single bases into a plant genome may produce desirable traits. Gene editing by CRISPR/Cas9 techniques frequently results in insertions–deletions (indels). Base editing allows precise single-nucleotide changes in the genome in the absence of double-strand breaks (DSBs) and donor repair templates (DRTs). Therefore, BEs have provided a new way of thinking about genome editing, and base editing techniques are currently being utilized to edit the genomes of many different organisms. As traditional breeding techniques and modern molecular breeding technologies complement each other, various genome editing technologies have emerged. How to realize the greater potential of BE applications is the question we need to consider. Here, we explain various base editings such as CBEs, ABEs, and CGBEs. In addition, the latest applications of base editing technologies in agriculture are summarized, including crop yield, quality, disease, and herbicide resistance. Finally, the challenges and future prospects of base editing technologies are presented. The aim is to provide a comprehensive overview of the application of BE in crop breeding to further improve BE and make the most of its value.https://www.mdpi.com/1467-3045/45/2/59base editingCRISPR/Cas9plantgenome editingcrop improvement |
| spellingShingle | Yizhen Li Jing Liang Bufang Deng Yingli Jiang Jingyan Zhu Like Chen Min Li Juan Li Applications and Prospects of CRISPR/Cas9-Mediated Base Editing in Plant Breeding Current Issues in Molecular Biology base editing CRISPR/Cas9 plant genome editing crop improvement |
| title | Applications and Prospects of CRISPR/Cas9-Mediated Base Editing in Plant Breeding |
| title_full | Applications and Prospects of CRISPR/Cas9-Mediated Base Editing in Plant Breeding |
| title_fullStr | Applications and Prospects of CRISPR/Cas9-Mediated Base Editing in Plant Breeding |
| title_full_unstemmed | Applications and Prospects of CRISPR/Cas9-Mediated Base Editing in Plant Breeding |
| title_short | Applications and Prospects of CRISPR/Cas9-Mediated Base Editing in Plant Breeding |
| title_sort | applications and prospects of crispr cas9 mediated base editing in plant breeding |
| topic | base editing CRISPR/Cas9 plant genome editing crop improvement |
| url | https://www.mdpi.com/1467-3045/45/2/59 |
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