Comparative study on the <it>in vitro </it>and <it>in vivo </it>properties of two bovine herpesvirus-5 reference strains
<p>Abstract</p> <p>Background</p> <p>Bovine herpesvirus 5 (BoHV-5) is an alphaherpesvirus responsible for meningoencephalitis in young cattle and it is antigenically and genetically related to bovine herpesvirus 1. BoHV-5 outbreaks are sporadic and restricted in their g...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-06-01
|
| Series: | Acta Veterinaria Scandinavica |
| Online Access: | http://www.actavetscand.com/content/53/1/37 |
| _version_ | 1811294204212543488 |
|---|---|
| author | Thiry Julien Muylkens Benoît Delgado Fernando Kotsias Fiorella Del Médico Zajac María P Ladelfa María F Thiry Etienne Romera Sonia A |
| author_facet | Thiry Julien Muylkens Benoît Delgado Fernando Kotsias Fiorella Del Médico Zajac María P Ladelfa María F Thiry Etienne Romera Sonia A |
| author_sort | Thiry Julien |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Bovine herpesvirus 5 (BoHV-5) is an alphaherpesvirus responsible for meningoencephalitis in young cattle and it is antigenically and genetically related to bovine herpesvirus 1. BoHV-5 outbreaks are sporadic and restricted in their geographical distribution, being mostly detected in the Southern hemisphere. The N569 and A663 strains are prototypes of the "a" and "b" subtypes of BoHV-5, however, scarce information about their <it>in vitro </it>and <it>in vivo </it>properties is currently available.</p> <p>Methods</p> <p>For the <it>in vitro </it>comparison between BoHV-5 A663 and N569 strains, viral growth kinetics, lysis and infection plaque size assays were performed. Additionally, an experimental infection of cattle with BoHV-5 A663 and N569 strains was carried out. Viral excretion, development of neurological signs, presence of specific antibodies in serum and nasal swabs and presence of latent BoHV-5 DNA in trigeminal ganglion, were analyzed. Histopathological examination of samples belonging to inoculated animals was also performed.</p> <p>Results</p> <p>The lytic capacity and the cell-to-cell spread was lower for the A663 strain compared to the N569 strain, however, the production of total infectious viral particles was similar between both strains. Concerning the <it>in vivo </it>properties, the A663 and N569 strains are able to induce similar degrees of pathogenicity in cattle.</p> <p>Conclusions</p> <p>Our results show that the A663 strain used in this study is less adapted to <it>in vitro </it>replication in MDBK cells than the N569 strain and, although slight differences were observed, both strains are able to induce a similar degree of virulence in the natural host.</p> |
| first_indexed | 2024-04-13T05:13:55Z |
| format | Article |
| id | doaj.art-a5461449ad704b88b1821c88ad28ae40 |
| institution | Directory Open Access Journal |
| issn | 1751-0147 |
| language | English |
| last_indexed | 2024-04-13T05:13:55Z |
| publishDate | 2011-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Acta Veterinaria Scandinavica |
| spelling | doaj.art-a5461449ad704b88b1821c88ad28ae402022-12-22T03:00:57ZengBMCActa Veterinaria Scandinavica1751-01472011-06-015313710.1186/1751-0147-53-37Comparative study on the <it>in vitro </it>and <it>in vivo </it>properties of two bovine herpesvirus-5 reference strainsThiry JulienMuylkens BenoîtDelgado FernandoKotsias FiorellaDel Médico Zajac María PLadelfa María FThiry EtienneRomera Sonia A<p>Abstract</p> <p>Background</p> <p>Bovine herpesvirus 5 (BoHV-5) is an alphaherpesvirus responsible for meningoencephalitis in young cattle and it is antigenically and genetically related to bovine herpesvirus 1. BoHV-5 outbreaks are sporadic and restricted in their geographical distribution, being mostly detected in the Southern hemisphere. The N569 and A663 strains are prototypes of the "a" and "b" subtypes of BoHV-5, however, scarce information about their <it>in vitro </it>and <it>in vivo </it>properties is currently available.</p> <p>Methods</p> <p>For the <it>in vitro </it>comparison between BoHV-5 A663 and N569 strains, viral growth kinetics, lysis and infection plaque size assays were performed. Additionally, an experimental infection of cattle with BoHV-5 A663 and N569 strains was carried out. Viral excretion, development of neurological signs, presence of specific antibodies in serum and nasal swabs and presence of latent BoHV-5 DNA in trigeminal ganglion, were analyzed. Histopathological examination of samples belonging to inoculated animals was also performed.</p> <p>Results</p> <p>The lytic capacity and the cell-to-cell spread was lower for the A663 strain compared to the N569 strain, however, the production of total infectious viral particles was similar between both strains. Concerning the <it>in vivo </it>properties, the A663 and N569 strains are able to induce similar degrees of pathogenicity in cattle.</p> <p>Conclusions</p> <p>Our results show that the A663 strain used in this study is less adapted to <it>in vitro </it>replication in MDBK cells than the N569 strain and, although slight differences were observed, both strains are able to induce a similar degree of virulence in the natural host.</p>http://www.actavetscand.com/content/53/1/37 |
| spellingShingle | Thiry Julien Muylkens Benoît Delgado Fernando Kotsias Fiorella Del Médico Zajac María P Ladelfa María F Thiry Etienne Romera Sonia A Comparative study on the <it>in vitro </it>and <it>in vivo </it>properties of two bovine herpesvirus-5 reference strains Acta Veterinaria Scandinavica |
| title | Comparative study on the <it>in vitro </it>and <it>in vivo </it>properties of two bovine herpesvirus-5 reference strains |
| title_full | Comparative study on the <it>in vitro </it>and <it>in vivo </it>properties of two bovine herpesvirus-5 reference strains |
| title_fullStr | Comparative study on the <it>in vitro </it>and <it>in vivo </it>properties of two bovine herpesvirus-5 reference strains |
| title_full_unstemmed | Comparative study on the <it>in vitro </it>and <it>in vivo </it>properties of two bovine herpesvirus-5 reference strains |
| title_short | Comparative study on the <it>in vitro </it>and <it>in vivo </it>properties of two bovine herpesvirus-5 reference strains |
| title_sort | comparative study on the it in vitro it and it in vivo it properties of two bovine herpesvirus 5 reference strains |
| url | http://www.actavetscand.com/content/53/1/37 |
| work_keys_str_mv | AT thiryjulien comparativestudyontheitinvitroitanditinvivoitpropertiesoftwobovineherpesvirus5referencestrains AT muylkensbenoit comparativestudyontheitinvitroitanditinvivoitpropertiesoftwobovineherpesvirus5referencestrains AT delgadofernando comparativestudyontheitinvitroitanditinvivoitpropertiesoftwobovineherpesvirus5referencestrains AT kotsiasfiorella comparativestudyontheitinvitroitanditinvivoitpropertiesoftwobovineherpesvirus5referencestrains AT delmedicozajacmariap comparativestudyontheitinvitroitanditinvivoitpropertiesoftwobovineherpesvirus5referencestrains AT ladelfamariaf comparativestudyontheitinvitroitanditinvivoitpropertiesoftwobovineherpesvirus5referencestrains AT thiryetienne comparativestudyontheitinvitroitanditinvivoitpropertiesoftwobovineherpesvirus5referencestrains AT romerasoniaa comparativestudyontheitinvitroitanditinvivoitpropertiesoftwobovineherpesvirus5referencestrains |