Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings
Abstract Pecan cultivation in Brazil has gained increasingly economic importance in the southern part of the country. The objective of this work was to identify the pathogen that causes root rot in Carya illinoinensis seedlings in the nursery, through morphological, molecular and pathogenicity tests...
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| Format: | Article |
| Language: | English |
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Universidade Federal Rural do Rio de Janeiro
2020-05-01
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| Series: | Floresta e Ambiente |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2179-80872020000200111&tlng=en |
| _version_ | 1828131204511039488 |
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| author | Tales Poletto Marlove Fátima Brião Muniz Vinícius Spolaor Fantinel Ricardo Harakava Jéssica Mengue Rolim |
| author_facet | Tales Poletto Marlove Fátima Brião Muniz Vinícius Spolaor Fantinel Ricardo Harakava Jéssica Mengue Rolim |
| author_sort | Tales Poletto |
| collection | DOAJ |
| description | Abstract Pecan cultivation in Brazil has gained increasingly economic importance in the southern part of the country. The objective of this work was to identify the pathogen that causes root rot in Carya illinoinensis seedlings in the nursery, through morphological, molecular and pathogenicity tests. Fungi from the genus Fusarium found in symptomatic roots of pecan seedlings were isolated in potato dextrose agar (PDA) culture medium and purified. Morphological characterization was performed in PDA and carnation leaf agar (CLA) media. For the molecular characterization, the genomic segments ITS (internal transcribed spaces), β-tub (beta-tubulin), and tef1-α (elongation fact 1-alpha) were sequenced. The pathogenicity test was performed on healthy seedlings in the greenhouse. The combination of morphological and molecular characters was fundamental for identification of the species, showing that the most suitable genes for identification were ITS and tef1-α. The causative agent of root rot in C. illinoinensis seedlings in the nursery was identified as Fusarium oxysporum. |
| first_indexed | 2024-04-11T16:44:09Z |
| format | Article |
| id | doaj.art-a548bd99550b4bf89ab5c8a930721369 |
| institution | Directory Open Access Journal |
| issn | 2179-8087 |
| language | English |
| last_indexed | 2024-04-11T16:44:09Z |
| publishDate | 2020-05-01 |
| publisher | Universidade Federal Rural do Rio de Janeiro |
| record_format | Article |
| series | Floresta e Ambiente |
| spelling | doaj.art-a548bd99550b4bf89ab5c8a9307213692022-12-22T04:13:36ZengUniversidade Federal Rural do Rio de JaneiroFloresta e Ambiente2179-80872020-05-0127210.1590/2179-8087.108917Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis SeedlingsTales Polettohttps://orcid.org/0000-0002-6162-4445Marlove Fátima Brião Munizhttps://orcid.org/0000-0001-7436-9589Vinícius Spolaor Fantinelhttps://orcid.org/0000-0002-0414-7486Ricardo Harakavahttps://orcid.org/0000-0003-1431-2665Jéssica Mengue Rolimhttps://orcid.org/0000-0003-2737-7599Abstract Pecan cultivation in Brazil has gained increasingly economic importance in the southern part of the country. The objective of this work was to identify the pathogen that causes root rot in Carya illinoinensis seedlings in the nursery, through morphological, molecular and pathogenicity tests. Fungi from the genus Fusarium found in symptomatic roots of pecan seedlings were isolated in potato dextrose agar (PDA) culture medium and purified. Morphological characterization was performed in PDA and carnation leaf agar (CLA) media. For the molecular characterization, the genomic segments ITS (internal transcribed spaces), β-tub (beta-tubulin), and tef1-α (elongation fact 1-alpha) were sequenced. The pathogenicity test was performed on healthy seedlings in the greenhouse. The combination of morphological and molecular characters was fundamental for identification of the species, showing that the most suitable genes for identification were ITS and tef1-α. The causative agent of root rot in C. illinoinensis seedlings in the nursery was identified as Fusarium oxysporum.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2179-80872020000200111&tlng=enetiologyroot rotpecan |
| spellingShingle | Tales Poletto Marlove Fátima Brião Muniz Vinícius Spolaor Fantinel Ricardo Harakava Jéssica Mengue Rolim Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings Floresta e Ambiente etiology root rot pecan |
| title | Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings |
| title_full | Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings |
| title_fullStr | Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings |
| title_full_unstemmed | Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings |
| title_short | Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings |
| title_sort | characterization and pathogenicity of fusarium oxysporum associated with carya illinoinensis seedlings |
| topic | etiology root rot pecan |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2179-80872020000200111&tlng=en |
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