Mitochondrial cellular organization and shape fluctuations are differentially modulated by cytoskeletal networks
Abstract The interactions between mitochondria and the cytoskeleton have been found to alter mitochondrial function; however, the mechanisms underlying this phenomenon are largely unknown. Here, we explored how the integrity of the cytoskeleton affects the cellular organization, morphology and mobil...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-31121-w |
| _version_ | 1797827588801953792 |
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| author | Agustina Belén Fernández Casafuz María Cecilia De Rossi Luciana Bruno |
| author_facet | Agustina Belén Fernández Casafuz María Cecilia De Rossi Luciana Bruno |
| author_sort | Agustina Belén Fernández Casafuz |
| collection | DOAJ |
| description | Abstract The interactions between mitochondria and the cytoskeleton have been found to alter mitochondrial function; however, the mechanisms underlying this phenomenon are largely unknown. Here, we explored how the integrity of the cytoskeleton affects the cellular organization, morphology and mobility of mitochondria in Xenopus laevis melanocytes. Cells were imaged in control condition and after different treatments that selectively affect specific cytoskeletal networks (microtubules, F-actin and vimentin filaments). We observed that mitochondria cellular distribution and local orientation rely mostly on microtubules, positioning these filaments as the main scaffolding of mitochondrial organization. We also found that cytoskeletal networks mold mitochondria shapes in distinct ways: while microtubules favor more elongated organelles, vimentin and actin filaments increase mitochondrial bending, suggesting the presence of mechanical interactions between these filaments and mitochondria. Finally, we identified that microtubule and F-actin networks play opposite roles in mitochondria shape fluctuations and mobility, with microtubules transmitting their jittering to the organelles and F-actin restricting the organelles motion. All our results support that cytoskeleton filaments interact mechanically with mitochondria and transmit forces to these organelles molding their movements and shapes. |
| first_indexed | 2024-04-09T12:50:44Z |
| format | Article |
| id | doaj.art-a548fed037b14f4781fe516182114f55 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T12:50:44Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a548fed037b14f4781fe516182114f552023-05-14T11:14:36ZengNature PortfolioScientific Reports2045-23222023-03-0113111210.1038/s41598-023-31121-wMitochondrial cellular organization and shape fluctuations are differentially modulated by cytoskeletal networksAgustina Belén Fernández Casafuz0María Cecilia De Rossi1Luciana Bruno2CONICET-Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Instituto de Cálculo (IC)CONICET-Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Instituto de Química Biológica (IQUIBICEN)CONICET-Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Instituto de Cálculo (IC)Abstract The interactions between mitochondria and the cytoskeleton have been found to alter mitochondrial function; however, the mechanisms underlying this phenomenon are largely unknown. Here, we explored how the integrity of the cytoskeleton affects the cellular organization, morphology and mobility of mitochondria in Xenopus laevis melanocytes. Cells were imaged in control condition and after different treatments that selectively affect specific cytoskeletal networks (microtubules, F-actin and vimentin filaments). We observed that mitochondria cellular distribution and local orientation rely mostly on microtubules, positioning these filaments as the main scaffolding of mitochondrial organization. We also found that cytoskeletal networks mold mitochondria shapes in distinct ways: while microtubules favor more elongated organelles, vimentin and actin filaments increase mitochondrial bending, suggesting the presence of mechanical interactions between these filaments and mitochondria. Finally, we identified that microtubule and F-actin networks play opposite roles in mitochondria shape fluctuations and mobility, with microtubules transmitting their jittering to the organelles and F-actin restricting the organelles motion. All our results support that cytoskeleton filaments interact mechanically with mitochondria and transmit forces to these organelles molding their movements and shapes.https://doi.org/10.1038/s41598-023-31121-w |
| spellingShingle | Agustina Belén Fernández Casafuz María Cecilia De Rossi Luciana Bruno Mitochondrial cellular organization and shape fluctuations are differentially modulated by cytoskeletal networks Scientific Reports |
| title | Mitochondrial cellular organization and shape fluctuations are differentially modulated by cytoskeletal networks |
| title_full | Mitochondrial cellular organization and shape fluctuations are differentially modulated by cytoskeletal networks |
| title_fullStr | Mitochondrial cellular organization and shape fluctuations are differentially modulated by cytoskeletal networks |
| title_full_unstemmed | Mitochondrial cellular organization and shape fluctuations are differentially modulated by cytoskeletal networks |
| title_short | Mitochondrial cellular organization and shape fluctuations are differentially modulated by cytoskeletal networks |
| title_sort | mitochondrial cellular organization and shape fluctuations are differentially modulated by cytoskeletal networks |
| url | https://doi.org/10.1038/s41598-023-31121-w |
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