Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002

Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002

Abstract Background Synechococcus sp. PCC 7002 (henceforth Synechococcus) is developing into a powerful synthetic biology chassis. In order to streamline the integration of genes into the Synechococcus chromosome, validation of neutral integration sites with optimization of the DNA transformation pr...

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Bibliographic Details
Main Authors: Anne Ilse Maria Vogel, Rahmi Lale, Martin Frank Hohmann-Marriott
Format: Article
Language:English
Published: BMC 2017-06-01
Series:Journal of Biological Engineering
Subjects:
Synechococcus sp. PCC7002
Neutral integration sites
Genetic engineering
Transformation
BioBrick
Cyanobacteria
Online Access:http://link.springer.com/article/10.1186/s13036-017-0061-8

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